Comparable sensitivity and specificity of nested PCR and single-stage PCR using a thermally activated DNA polymerase

BioTechniques

Volumn 24, Issue 2, 1998, Pages 222-224

  Zimmermann, Klaus ^a   Mannhalter, Josef W ^a  

^a BAXTER HEALTHCARE CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

ARTICLE; COMPARATIVE STUDY; DNA SEQUENCE; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE AMPLIFICATION; MONONUCLEAR CELL; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0031890956     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242bm11     Document Type: Article

References (6)

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2. Birch, D.E., L. Kolmodin, J. Wong, G.A. Zangenberg, M.A. Zoccoli, N. McKinney, K.K.Y. Young and W.J. Laird. 1996. Simplified hot start PCR. Nature 381:445-446.
3. Mullis, K.B. 1991. The polymerase chain reaction in an anemic mode: how to avoid cold oligodeoxyribonuclear fusion. PCR Methods Appl. 1:1-4.
4. Ratner, L., W. Haseltine, R. Patarca, K.J. Livak, B. Starcich, S.F. Josephs, E.R. Doran, J.A. Rafalski et al. 1985. Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature 313:277-284.
5. Zazzi, M., L. Romano, F. Peruzzi, S. Toneatto, A. De Milito, G. Botta and P.E. Valensin. 1993. Optimal conditions for detection of human immunodeficiency virus type 1 DNA by polymerase chain reaction with nested primers. Mol. Cell. Probes 7:431-437.
6. Zimmermann, K., K. Pischinger and J.W. Mannhalter. 1994. Nested primer PCR detection limits of HIV-1 in the background of increasing numbers of lysed cells. BioTechniques 17:18-20.