The hydrogenosomal malic enzyme from the anaerobic fungus Neocallimastix frontalis is targeted to mitochondria of the methylotrophic yeast Hansenula polymorpha

Current Genetics

Volumn 33, Issue 2, 1998, Pages 131-135

  Van Der Giezen, Mark ^a,b   Kiel, Jan A K W ^a   Sjollema, Klaas A ^a   Prins, Rudolf A ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b DEPARTMENT OF LIFE SCIENCES   (United Kingdom)

Author keywords

Heterologous expression; Hydrogenosome; Neocallimastix frontalis; Targeting signal

Indexed keywords

FUNGAL PROTEIN; MALATE DEHYDROGENASE (DECARBOXYLATING); SIGNAL PEPTIDE;

AMINO TERMINAL SEQUENCE; ANAEROBIC GROWTH; ARTICLE; FUNGUS; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; YEAST;

ANAEROBIOSIS; CHYTRIDIOMYCOTA; MALATE DEHYDROGENASE; MICROBODIES; MICROSCOPY, ELECTRON; MITOCHONDRIA; PICHIA; PROTEIN SORTING SIGNALS;

FUNGI; NEOCALLIMASTIX FRONTALIS; PICHIA ANGUSTA;

EID: 0031890908     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050318     Document Type: Article

References (27)

1. Benchimol M, Durand R, Almeida JCA (1997) A double membrane surrounds the hydrogenosomes of the anaerobic fungus Neocallimastix frontalis. FEMS Microbiol Lett 154:277-282
2. Biagini GA, Finlay BJ, Lloyd D (1997) Evolution of the hydrogenosome. FEMS Microbiol Lett 155:133-140
3. Bradley PJ, Lahti CJ, Plümper E, Johnson PJ (1997) Targeting and translocation of proteins into the hydrogenosome of the protist Trichomonas: similarities with mitochondrial protein import. EMBO J 16:3484-3493
4. Brondijk THC, Durand R, van der Giezen M, Gottschal JC, Prins RA, Fèvre M (1996) scsB, a cDNA encoding the hydrogenosomal protein β-succinyl-CoA synthetase from the anaerobic fungus Neocallimastix frontalis. Mol Gen Genet 253:315-323
5. Cavalier-Smith T (1987) The simultaneous symbiotic origin of mitochondria, chloroplasts, and microbodies. Ann NY Acad Sci 503:55-71
6. Douma AC, Veenhuis M, de Koning W, Evers ME, Harder W (1985) Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha. Arch Microbiol 143:237-243
7. Durand R, Rascle C, Fischer M, Fèvre M (1997) Transient expression of the β-glucuronidase gene after biolistic transformation of the anaerobic fungus Neocallimastix frontalis. Curr Genet 31:158-161
8. Embley TM, Horner DA, Hirt RP (1997) Anaerobic eukaryote evolution: hydrogenosomes as biochemically modified mitochondria? Trends Ecol Evol 12:437-441
9. Faber KN, Haima P, de Hoop MJ, Harder W, Veenhuis M, AB G (1993) Peroxisomal amine oxidase of Hansenula polymorpha does not require its SRL-containing C-terminal sequence for targeting. Yeast 9:331-338
10. Faber KN, Haima P, Harder W, Veenhuis M, AB G ( 1994) Highly-efficient electrotransformation of the yeast Hansenula polymorpha. Curr Genet 25:305-310
11. Finlay BJ, Fenchel T (1989) Hydrogenosomes in some anaerobic protozoa resemble mitochondria. FEMS Microbiol Lett 65: 311-314
12. Giezen M van der, Rechinger KB, Svendsen I, Durand R, Hirt RP, Fèvre M, Embley TM, Prins RA (1997 a) A mitochondrial-like targeting signal on the hydrogenosomal malic enzyme from the anaerobic fungus Neocallimastix frontalis: evidence for the hypothesis that hydrogenosomes are modified mitochondria. Mol Microbiol 23:11-21
13. Giezen M van der, Sjollema KA, Artz RRE, Alkema W, Prins RA (1997 b) Hydrogenosomes in the anaerobic fungus Neocallimastix frontalis have a double membrane but lack an associated organelle genome. FEBS Lett 408:147-150
14. Gleeson MAG, Sudberry PE (1988) Genetic analysis in the methylotrophic yeast Hansenula polymorpha. Yeast 4:293-303
15. Heijne G von, Steppuhn J, Herrmann RG (1989) Domain structure of mítochondrial and chloroplast targeting peptides. Eur J Biochem 180:535-545
16. Johnson PJ, D'Oliveira CE, Gorrell TE, Müller M (1990) Molecular analysis of the hydrogenosomal ferredoxin of the anaerobic protist Trichomonas vaginalis. Proc Natl Acad Sci USA 87: 6097-6101
17. Keizer-Gunnink I, Roggenkamp R, Harder W, Veenhuis M (1992) Location of catalase in crystalline peroxisomes of methanol-grown Hansenula polymorpha. FEMS Microbiol Lett 93:7-12
18. Komori M, Rasmussen SW, Kiel JAKW, Baerends RJ, Cregg JM, Van der Klei IJ, Veenhuis M (1997) The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis. EMBO J 16:44-53
19. Kyhse-Anderson J (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10:203-209
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
21. Lindmark DG, Müller M (1973) Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritichomonas foetus, and its role in pyruvate metabolism. J Biol Chem 248:7724-7728
22. Marvin-Sikkema FD, Lahpor GA, Kraak MN, Gottschal JC, Prins RA (1992) Characterization of an anaerobic fungus from Ilama faeces. J Gen Microbiol 138:2235-2241
23. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
24. Slot JW and Geuze HJ (1984) Gold markers for single and double immunolabeling of ultrathin cryo-sections. In: Polak JM, Varndell JM (eds) Immunolabeling for electron microscopy. Elsevier Scientific Publishers, Amsterdam, pp 129-142
25. Veenhuis M, Keizer I, Harder W (1979) Characterization of peroxisomes in glucose-grown Hansenula polymorpha and their development after the transfer of cells into methanol-containing media. Arch Microbiol 120:167-175
26. Waterham HR, Titorenko VI, Haima P, Cregg JM, Harder W, Veenhuis M (1994) The Hansenula polymorpha PERI gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy and amino-terminal targeting signals. J Cell Biol 127:737-749
27. Whatley JM, John P, Whatley FR (1979) From extracellular to intracellular: the establishment of mitochondria and chloroplasts. Proc R Soc Lond B 204:165-187