Optimization of bacteriocin release protein (BRP)-mediated protein release by Escherichia coli: Random mutagenesis of the pCloDF13-derived BRP gene to uncouple lethality and quasi-lysis from protein release

Applied and Environmental Microbiology

Volumn 64, Issue 2, 1998, Pages 392-398

  Van Der Wal, Fimme J ^a   Koningstein, G ^a   Ten Hagen, C M ^a   Oudega, Bauke ^a   Luirink, Joen ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOCIN; BACTERIOCIN RELEASE PROTEIN; BETA LACTAMASE; CLOACIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; COLONY FORMING UNIT; ESCHERICHIA COLI; GROWTH INHIBITION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN SECRETION;

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; UNIDENTIFIED BACTERIOPHAGE;

EID: 0031890506     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.2.392-398.1998     Document Type: Article

References (31)

1. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Boyko, W. L., and R. E. Ganshow. 1982. Rapid identification of Escherichia coli transformed by pBR322 carrying inserts at the PstI site. Anal. Biochem. 122:85-88.
3. Cavard, D. 1994. Truncated mutants of the colicin A lysis protein are acylated and processed when overproduced. FEMS Microbiol. Lett. 117:169-174.
4. Gaillard, C., and F. Strauss. 1990. Ethanol precipitation of DNA with linear polyacrylamide as carrier. Nucleic Acids Res. 18:378.
5. Gennity, J. M., H. Kim, and M. Inouye. 1992. Structural determinants in addition to the amino-terminal sorting sequence influence membrane localization of Escherichia coli lipoproteins. J. Bacteriol. 174:2095-2101.
6. 2-terminal of lipoprotein are sufficient for its modification, processing, and localization in the outer membrane of Escherichia coli. J. Biol. Chem. 259: 463-467.
7. Howard, S. P., D. Cavard, and C. Lazdunski. 1989. Amino acid sequence and length requirements for assembly and function of the colicin A lysis protein. J. Bacteriol. 171:410-418.
8. Hsiung, H. M., A. Cantrell, J. Luirink, B. Oudega, A. J. Veros, and G. W. Becker. 1989. Use of bacteriocin release protein in Escherichia coli for excretion of human growth hormone into the culture medium. Bio/Technology 7:267-271.
9. Inouye, S., G. Duffaud, and M. Inouye. 1986. Structural requirement at the cleavage site for efficient processing of the lipoprotein secretory precursor of Escherichia coli. J. Biol. Chem. 261:10970-10975.
10. Kuipers, O. P., H. J. Boot, and W. M. de Vos. 1991. Improved site directed mutagenesis method using PCR. Nucleic Acids Res. 19:4558.
11. Luirink, J., D. M. Clark, J. Ras, E. J. Verschoor, F. Stegehuis, F. K. de Graaf, and B. Oudega. 1989. pCloDF13-encoded bacteriocin release proteins with shortened carboxyl-terminal segments are lipid modified and processed and function in release of cloacin DF13 and apparent host cell lysis. J. Bacteriol. 171:2673-2679.
12. Luirink, J., B. Duim, J. W. L. de Gier, and B. Oudega. 1991. Functioning of the stable signal peptide of the pCloDF13-encoded bacteriocin release protein. Mol. Microbiol. 5:393-399.
13. Luirink, J., S. Hayashi, H. C. Wu, M. M. Kater, F. K. de Graaf, and B. Oudega. 1988. Effect of a mutation preventing lipid modification on the localization of the pCloDF13-encoded bacteriocin release protein (BRP) and on the release of cloacin DF13. J. Bacteriol. 170:4153-4160.
14. Luirink, J., S. High, H. Wood, A. Giner, D. Tollervey, and B. Dobberstein. 1992. Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex. Nature 359:741-743.
15. Luirink, J., C. van der Sande, J. Tommassen, E. Veitkamp, F. K. de Graaf, and B. Oudega. 1986. Effects of divalent cations and of phospholipase A activity on excretion of cloacin DF13 and lysis of host cells. J. Gen. Microbiol. 132:825-834.
16. Luirink, J., T. Watanabe, H. C. Wu, F. Stegehuis, F. K. de Graaf, and B. Oudega. 1987. Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of β-lactamase. J. Bacteriol. 169:2245-2250.
17. MacIntyre, S., M. L. Eschbach, and B. Mutschler. 1990. Export incompatibility of N-terminal basic residues in a mature polypeptide of Escherichia coli can be alleviated by optimising the signal peptide. Mol. Gen. Genet. 221:466-474.
18. Mandel, M., and A. Higa. 1970. Calcium dependent bacteriophage DNA infection. J. Mol. Biol. 53:159-162.
19. Miller, J. H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Mol, O. 1996. Biosynthesis of K88 fimbriae of Escherichia coli: interaction of periplasmic chaperone FaeE and outer membrane usher FaeD with various K88 subunits. Ph.D. thesis. Vrije Universiteit, Amsterdam, The Netherlands.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
23. Siderovski, D. P., and T. W. Mak. 1993. RAMHA: a PC-based Monte-Carlo simulation of random saturation mutagenesis. Comput. Biol. Med. 23:463-474.
24. 2+ transport. Antonie Leeuwenhoek 67:260-278.
25. van der Wal, F. J., C. M. ten Hagen, B. Oudega, and J. Luirink. 1995. Application of the pCloDF13 bacteriocin release protein in the release of heterologous proteins by Escherichia coli: production of plant α-galactosidase. Biotechnol. Lett. 17:815-820.
26. van der Wal, F. J., C. M. ten Hagen, B. Oudega, and J. Luirink. 1995. Optimization of bacteriocin-release-protein-induced protein release by Escherichia coli: extracellular production of the periplasmic molecular chaperone FaeE. Appl. Microbiol. Biotechnol. 44:459-465.
27. van der Wal, F. J., J. Luirink, and B. Oudega. 1995. Bacteriocin release proteins: mode of action, structure and biotechnological application. FEMS Microbiol. Rev. 17:381-399.
28. van der Wal, F. J., Q. Valent, C. M. ten Hagen-Jongman, F. K. de Graaf, B. Oudega, and J. Luirink. 1994. Stability and function of the stable signal peptide of pCloDF13 derived bacteriocin release protein. Microbiology 140: 369-378.
29. Weichenhan, D. 1991. Fast recovery of DNA from agarose gels by centrifugation through blotting paper. Trends Genet. 7:109.
30. Yamaguchi, K., F. Yu, and M. Inouye. 1988. A single amino acid determinant of the membrane localization of lipoproteins in Escherichia coli. Cell 53:423-432.
31. Yamane, K., and S. Mizushima. 1988. Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 263:19690-19696.