PCR-based method for the introduction of mutations in genes cloned and expressed in vaccinia virus

BioTechniques

Volumn 24, Issue 2, 1998, Pages 308-313

  Del Mar Lorenzo, María ^a   Blasco, Rafael ^a,b  

^a INIA   (Spain)

^b Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; SPAIN; VACCINIA VIRUS; VIRUS GENE;

AEQUOREA VICTORIA; ANIMALIA; MAMMALIA; VACCINIA; VACCINIA VIRUS; VICTORIA;

EID: 0031889865     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242rr03     Document Type: Article

References (16)

1. Blasco, R. and B. Moss. 1991. Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-dalton outer envelope antigen. J. Virol. 65:5910-5920.
2. Blasco, R. and B. Moss. 1992. Role of cell-associated enveloped vaccinia virus in cell-to-cell virus spread. J. Virol. 66:4170-4179.
3. Blasco, R. and B. Moss. 1995. Selection of recombinant vaccinia viruses on the basis of plaque formation. Gene 158:157-162.
4. CadweIl, R.C. and G.F. Joyce. 1994. Mutagenic PCR. PCR Methods Appl. 3:S136-S140.
5. Chiang, L.W. 1996. Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR). Methods Mol. Biol. 57:311-321.
6. Cubitt, A.B., R. Heim, S.R. Adams, A.E. Boyd, L.A. Gross and R.Y. Tsien. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:448-455.
7. Earl, P. and B. Moss. 1991. Expression of proteins in mammalian cells using vaccinia viral vectors, p. 16.15.1-16.19.9. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York.
8. Falkner, F.G. and B. Moss. 1990. Transient dominant selection of recombinant vaccinia viruses. J. Virol. 64:3108-3111.
9. Ge, L. and P. Rudolph. 1997. Simultaneous introduction of multiple mutations using overlap extension PCR. BioTechniques 22:28-30.
10. Heim, R., A.B. Cubitt and R.Y. Tsien. 1995. Improved green fluorescence [letter]. Nature 373:663-664.
11. Ho, S.N., H.D. Hunt, R.M. Horton, J.K. Pullen and L.R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
12. Kegler-Ebo, D.M., G.W. Polack and D. DiMaio. 1996. Use of codon cassette mutagenesis for saturation mutagenesis. Methods Mol. Biol. 57:297-310.
13. Kuipers, O.P. 1996. Random mutagenesis by using mixtures of dNTP and dITP in PCR. Methods Mol. Biol. 57:351-356.
14. Ropp, J.D., C.J. Donahue, D. Wolfgang-Kimball, J.J. Hooley, J.Y. Chin, R.A. Hoffman, R.A. Cuthbertson and K.D. Bauer. 1995. Aequorea green fluorescent protein analysis by flow cytometry. Cytometry 27:309-317.
15. Wu, G.Y., D.J. Zou, T. Koothan and H.T. Cline. 1995. Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins. Neuron 74:681-684.
16. Zhang, Y. and B. Moss. 1991. lnducer-dependent conditional-lethal mutant animal viruses. Proc. Natl. Acad. Sci. USA 88:1511-1515.