Preparation of PCR products for DNA sequencing

BioTechniques

Volumn 24, Issue 2, 1998, Pages 314-317

  Leonard, Jack T ^a,d   Grace, M B ^b   Buzard, G S ^c   Mullen, M J ^a   Barbagallo, C B ^a  

^a Millipore   (United States)

^b NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

^d Analytical Products Division   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; CENTRIFUGATION; DNA SEQUENCE; DNA TEMPLATE; POLYMERASE CHAIN REACTION;

EID: 0031889772     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242pf01     Document Type: Article

References (5)

1. 1.Amersham (formerly United States Biochemical). 1994. Sequenase PCR Product Sequencing Kit (Product No. 70170): Step-By-Step Protocols. Cleveland, OH.
2. Hultzman, T., S. Ståhl, E. Homes and M. Uhlén. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17:4937-4946.
3. Krowczynska, A.M. and M.B. Henderson. 1992. Efficient purification of PCR products using ultrafiltration. BioTechniques 13:286-289.
4. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
5. Werle, E., C. Schneider, M. Renner, M. Völker and W. Fiehn. 1994. Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Res. 22:4354-4355.