Increased levels of elastase and α1-antitrypsin in sputum of asthmatic patients

American Journal of Respiratory and Critical Care Medicine

Volumn 157, Issue 2, 1998, Pages 505-511

  Vignola, Antonio M ^a   Bonanno, Anna ^b   Mirabella, Angela ^b   Riccobono, Loredana ^b   Mirabella, Franco ^b   Profita, Mirella ^b   Bellia, Vincenzo ^b   Bousquet, Jean ^b   Bonsignore, Giovanni ^b  

^a CNR   (Italy)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN; ELASTASE;

ADULT; AIRWAY OBSTRUCTION; ARTICLE; ASTHMA; CHRONIC BRONCHITIS; CIGARETTE SMOKING; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; PRIORITY JOURNAL; SALIVA ANALYSIS; SPUTUM ANALYSIS;

EID: 0031888346     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/ajrccm.157.2.9703070     Document Type: Article

References (35)

1. Senior, R. M., N. L. Connolly, J. D. Cury, H. G. Welgus, and E. J. Campbell. 1989. Elastin degradation by human alveolar macrophages: a prominent role of metalloproteinase activity. Am. Rev. Respir. Dis. 139:1251-1256.
2. Lungarella, G., R. Menegazzi, C. Gardi, P. Spessotto, M. M. de-Santi, P. Bertoncin, P. Patriarca, P. Calzoni, and G. Zabucchi. 1992. Identification of elastase in human eosinophils: immunolocalization, isolation, and partial characterization. Arch. Biochem. Biophys. 292:128-135.
3. Cohen, A. B., and M. Rossi. 1983. Neutrophils in normal lungs. Am. Rev. Respir. Dis. 127:S3-S9.
4. Travis, J., and G. S. Salvesen. 1983. Human plasma proteinase inhibitors. Annn. Rev. Biochem. 52:655-709.
5. Stockley, R. A. 1994. The role of proteinases in the pathogenesis of chronic bronchitis. Am. J. Respir. Crit. Care Med. 150:S109-S113.
6. Janoff, A. 1985. Elastases and emphysema: current assessment of the protease-antiprotease hypothesis. Am. Rev. Respir. Dis. 132:417-433.
7. Thurlbeck, W. M. 1990. Pathology of chronic airflow obstruction. Chest 97:6S-10S.
8. Bousquet, J., P. Chanez, J. Y. Lacoste, R. White, P. Vie, P. Godard, and F. B. Michel. 1992. Asthma: a disease remodeling the airways. Allergy 47:3-11.
9. Roche, W. R., R. Beasley, J. H. Williams, and S. T. Holgate. 1989. Subepithelial fibrosis in the bronchi of asthmatics. Lancet 1:520-524.
10. Bousquet, J., J. Lacoste, P. Chanez, P. Vie, P. Godard, and F. Michel. 1996. Bronchial elastic fibers in normal subjects and asthmatic patients. Am. J. Respir. Crit. Care Med. 153:1648-1653.
11. American Thoracic Society. 1962. Definitions and classifications of chronic bonchitis, asthma and emphysema. Am. Rev. Respir. Dis. 85:762-768.
12. American Thoracic Society. 1987. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease (COPD) and asthma. Am. Rev. Respir. Dis. 136:225-244.
13. Fahy, J. V., D. J. Steiger, J. Liu, C. B. Basbaum, W. E. Finkbeiner, and H. A. Boushey. 1993. Markers of mucus secretion and DNA levels in induced sputum from asthmatic and from healthy subjects. Am. Rev. Respir. Dis. 147:1132-1137.
14. Neumann, S., G. Gunzer, N. Hennrich, and H. Lang. 1984. "PMN-elastase assay": enzyme immunoassay for human polymorphonuclear elastase complexed with alpha 1-proteinase inhibitor. J. Clin. Chem. Clin. Biochem. 22:693-697.
15. Fujita, J., N. L. Nelson, D. M. Daughton, C. A. Dobry, J. R. Spurzem, S. Irino, and S. I. Rennard. 1990. Evaluation of elastase and antielastase balance in patients with chronic bronchitis and pulmonary emphysema. Am. Rev. Respir. Dis. 142:57-62.
16. Betsuyaku, T., A. Yoshioka, M. Nishimura, K. Miyamoto, T. Kondo, and Y. Kawakami. 1995. Neutrophil elastase associated with alveolar macrophages from older volunteers. Am. J. Respir. Crit. Care Med. 151: 436-442.
17. Ritchie, R., C. Alper, J. Gravex, N. Pearson, and C. Larson. 1973. Automated quantitation of proteins in serum and other biologic fluids. Am. J. Clin. Pathol. 59:151-159.
18. Malech, H. L., and J. I. Gallin. 1987. Current concepts: immunology. Neutrophils in human diseases. N. Engl. J. Med. 317:687-694.
19. Cosio, M., H. Ghezzo, J. Hogg, R. Corbin, M. Loveland, J. Dossman, and P. Macklem. 1977. The relations between structural changes in small airways and pulmonary function tests. N. Engl. J. Med. 298: 1277-1281.
20. Fahy, J. V., K. W. Kim, J. Liu, and H. A. Boushey. 1995. Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation. J. Allergy Clin. Immunol. 95:843-852.
21. Maestrelli, P., M. Saetta, A. Di Stefano, P. G. Calcagni, G. Turato, M. P. Ruggieri, A. Roggeri, C. E. Mapp, and L. M. Fabbri. 1995. Comparison of leukocyte counts in sputum, bronchial biopsies, and bronchoalveolar lavage. Am. J. Respir. Crit. Care Med. 152:1926-1931.
22. Lacoste, J. Y., J. Bousquet, P. Chanez, T. Van-Vyve, J. Simony-Lafontaine, N. Lequeu, P. Vic, I. Enander, P. Godard, and F. B. Michel. 1993. Eosinophilic and neutrophilic inflammation in asthma, chronic bronchitis, and chronic obstructive pulmonary disease. J. Allergy Clin. Immunol. 92:537-548.
23. Jackson, A. H., S. L. Hill, S. C. Afford, and R. A. Stockley. 1984. Sputum sol-phase proteins and elastase activity in patients with cystic fibrosis. Eur. J. Respir. Dis. 65:114-124.
24. Vissers, M. C., P. M. George, I. C. Bathurst, S. O. Brennan, and C. C. Winterbourn. 1988. Cleavage and inactivation of alpha 1-antitrypsin by metalloproteinases released from neutrophils. J. Clin. Invest. 82:706-711.
25. Snider, G. L. 1985. Distinguishing among asthma, chronic bronchitis, and emphysema. Chest 87:35S-39S.
26. Sommerhoff, C. P., and W. E. Finkbeiner. 1990. Human tracheobronchial submucosal gland cells in culture. Am. J. Respir. Cell Mol. Biol. 2:41-50.
27. Amitani, R., R. Wilson, A. Rutman, R. Read, C. Ward, D. Burnett, R. A. Stockley, and P. J. Cole. 1991. Effects of human neutrophil elastase and Pseudomonas aeruginosa proteinases on human respiratory epithelium. Am. J. Respir. Cell Mol. Biol. 4:26-32.
28. Nakamura, H., K. Yoshimura, N. G. McElvaney, and R. G. Crystal. 1992. Neutrophil elastase in respiratory epithelial lining fluid of individuals with cystic fibrosis induces interleukin-8 gene expression in a human bronchial epithelial cell line. J. Clin. Invest. 89:1478-1484.
29. Suzuki, T., W. Wang, J. T. Lin, K. Shirato, H. Mitsuhashi, and H. Inoue. 1996. Aerosolized human neutrophil elastase induces airway constriction and hyperresponsiveness with protection by intravenous pretreatment with half-length secretory leukoprotease inhibitor. Am. J. Respir. Crit. Care Med. 153:1405-1411.
30. Chetty, A., P. Davis, and M. Infeld. 1995. Effect of elastase on the directional migration of lung fibroblasts within a three-dimensional collagen matrix. Exp. Lung Res. 21:889-899.
31. Wright, R. 1961. Elastic tissue of normal and emphysematous lungs: a tridimentional histologic study. Am. J. Pathol. 34:355-363.
32. McCarthy, D. S., and M. Sigurdson. 1980. Lung elastic recoil and reduced airflow in clinically stable asthma. Thorax 35:298-302.
33. Brackel, H., J. Bogaard, and F. Kerrebijn. 1990. The compressibility of central airways in healthy subjects and patients with severe asthma (abstract). Am. Rev. Respir. Dis. 141:A848.
34. Wilson, J. W., X. Li, and M. C. Pain. 1993. The lack of distensibility of asthmatic airways. Am. Rev. Respir. Dis. 148:806-809.
35. Burnett, D., A. Chamba, S. L. Hill, and R. A. Stockley. 1987. Neutrophils from subjects with chronic obstructive lung disease show enhanced chemotaxis and extracellular proteolysis. Lancet 2:1043-1046.