Rapid and efficient purification of actin from nonmuscle sources

Cell Motility and the Cytoskeleton

Volumn 39, Issue 2, 1998, Pages 166-171

  Schafer, Dorothy A ^a   Jennings, Phillip B ^a   Cooper, John A ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Actin isoforms; Nonmuscle sources; Purification

Indexed keywords

ACTIN; BETA ACTIN; CELLULOSE; MUSCLE PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL MOTILITY; CHICKEN; CHROMATOGRAPHY; CYTOSKELETON; EMBRYO; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN STRUCTURE;

ACTINS; ANIMALS; BRAIN; CATTLE; CHICKENS; ERYTHROCYTES;

ANIMALIA; BOVINAE; GALLUS GALLUS;

EID: 0031888137     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1998)39:2<166::AID-CM7>3.0.CO;2-4     Document Type: Article

References (30)

1. Allen, P.O., Shuster, C.B., Kas, J., Chaponnier, C., Janmey, P.A., and Herman, I.M. (1996): Phalloidin binding and Theological differences among actin isoforms. Biochemistry 35:14062-14069.
2. Cano, M.L., Cassimeris, L., Fechheimer, M., and Zigmond, S.H. (1992): Mechanisms responsible for F-actin stabilization after lysis of polymorphonuclear leukocytes. J. Cell Biol. 116:1123- 1134.
3. Casella, J.F., Barron-Casella, E.A., and Torres, M.A. (1995): Quantitation of Cap Z in conventional actin preparations and methods for further purification of actin. Cell Motil. Cytoskeleton 30:164-170.
4. Gordon, D.J., Eisenberg, E., and Korn, E.D. (1976): Characterization of cytoplasmic actin isolated from Acanthamoeba castellanii by a new method. J. Biol. Chem. 251:4778-4786.
5. Gordon, D.J., Boyer, J.L., and Korn, E.D. (1977): Comparative biochemistry of non-muscle actins. J. Biol. Chem. 252:8300- 8309.
6. Gunning, P., Weinberger, R., and Jeffrey, P. (1997): Actin and tropomyosin isoforms in morphogenesis. Anat. Embryol. 195: 311-315.
7. Hart, M.C., Korshunova, Y.O., and Cooper, J.A. (1997): Vertebrates have conserved capping protein a isoforms with specific expression patterns. Cell Motil. Cytoskeleton. 38:120-132.
8. Herman, I.M. (1993): Actin isoforms. Curr. Opin. Cell Biol. 5:48-55.
9. Janmey, P.A., and Chaponnier, C. (1995): Medical aspects of the actin Cytoskeleton. Curr. Opin. Cell Biol. 7:111-117.
10. Just, I., Wollenberg, P., Moss, J., and Aktories, K. (1994): Cysteine- specific ADP-ribosylation of actin. Eur. J. Biochem. 221:1047- 1054.
11. Kron, S.J., Drubin, D.G., Botstein, D., and Spudich, J.A. (1992): Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin. Proc. Natl. Acad Sci. U.S.A. 89:4466-4470.
12. Kumar, A., Crawford, K., Close, L., Madison, M., Lorenz, J., Doetschman, T., Pawlowski, S., Duffy, J., Neumann, J., Robbins, J., Boivin, G.P., O'Toole, B.A., and Lessard, J.L. (1997): Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin. Proc. Natl. Acad. Sci. U.S.A. 94:4406- 4411.
13. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
14. Lessard, J.L. (1988): Two monoclonal antibodies to actin: One muscle selective and one generally reactive. Cell Motil. Cytoskeleton 10:349-362.
15. Ohshima, S., Abe, H., and Obinata, T. (1989): Isolation of profilin from embryonic chicken skeletal muscle and evaluation of its interaction with different actin isoforms. J. Biochem. 105:855- 857.
16. Pinder, J.C., and Gratzer, W.B. (1982): Investigation of the actin deoxyribonuclease-I interaction using a pyrene-conjugated actin derivative. Biochemistry 21:4886-4890.
17. Pinder, J.C., Sleep, J.A., Bennett, P.M., and Gratzer, W.B. (1995): Concentrated Tris solutions for the preparation, depolymerization, and assay of actin: Application to erythroid actin. Anal. Biochem. 225:291-295.
18. Prassler, J., Stocker, S., Marriott, G., Heidecker, M., Kellerman, J., and Gerisch, G. (1997): Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes. Mol. Biol. Cell. 8:83-95.
19. Puszkin, S., Maimon, J., and Puszkin, E. (1978): Erythrocyte actin and spectrin. Interactions with muscle contractile and regulatory proteins. Biochim. Biophys. Acta 513:205-220.
20. Ronnov-Jessen, L., and Petersen, O.W. (1996): A function for filamentous alpha-smooth muscle actin: Retardation of motility in fibroblasts. J. Cell Biol. 134:67-80.
21. Rubenstein, P.A., and Spudich, J.A. (1977): Actin microheterogeneity in chick embryo fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 74:120-123.
22. Schafer, D.A., Korshunova, Y.O., Schroer, T.A., and Cooper, J.A. (1994): Differential localization and sequence analysts of capping protein β-subunit isoforms of vertebrates. J. Cell Biol. 127:453-465.
23. Schafer, D.A., Jennings, P.B., and Cooper, J.A. (1996): Dynamics of capping protein and actin assembly in vitro: Uncapping barbed ends by polyphosphoinositides. J. Cell Biol. 135:169-179.
24. Schaier, S.R. (1992): Purification and characterization of platelet actin, actin-binding protein, and α-actinin. Methods Enzymol. 215: 58-77.
25. Segura, M., and Lindberg, U. (1984): Separation of non-muscle isoactins in the free form or as profilactin complexes. J. Biol. Chem. 259:3949-3954.
26. Shuster, C.B., and Herman, I.M. (1995): Indirect association of ezrin with F-actin-isoform specificity and calcium sensitivity. J. Cell Biol. 128:837-848.
27. Spudich, J.A., and Watt, S. (1971): The regulation of rabbit skeletal muscle contraction. J. Biol. Chem. 246:4866-4871.
28. Towbin, H., Staehlin, T., and Gordon, J. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76:4350-4354.
29. Weber, A., Nachmias, V.T., Pennise, C., Pring, M., and Safer, D. (1992): Interaction of thymosin b4 with muscle and platelet actin: Implications for actin sequestration in resting platelets. Biochemistry 31:6179-6185.
30. Weir, J.P., and Frederiksen, D.W. (1982): Preparation of cytoplasmic actin. Methods Enzymol. 85:371-373.