An improved procedure to quantify tumour vascularity using true colour image analysis. Comparison with the manual hot-spot procedure in a human melanoma xenograft model

Journal of Pathology

Volumn 184, Issue 2, 1998, Pages 136-143

  Van Der Laak, Jeroen A W M ^a   Westphal, Johan R ^a   Schalkwijk, Lia J M ^a   Pahlplatz, Martin M M ^a   Ruiter, Dirk J ^a   De Waal, Robert M W ^a   De Wilde, Peter C M ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Digital image analysis; Immunohistochemistry; Morphometric study; Tumour angiogenesis

Indexed keywords

ARTICLE; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; IMMUNOHISTOCHEMISTRY; MELANOMA; OBSERVER VARIATION; PRIORITY JOURNAL; PROGNOSIS; TUMOR VASCULARIZATION; XENOGRAFT;

ANIMALS; COLOR; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOENZYME TECHNIQUES; MELANOMA, EXPERIMENTAL; MICE; MICE, INBRED BALB C; NEOPLASM TRANSPLANTATION; NEOVASCULARIZATION, PATHOLOGIC; OBSERVER VARIATION; PROGNOSIS; TRANSPLANTATION, HETEROLOGOUS; TUMOR CELLS, CULTURED;

EID: 0031888022     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199802)184:2<136::AID-PATH970>3.0.CO;2-9     Document Type: Article

References (22)

1. Weidner N. Intratumor microvessel density as a prognostic factor in cancer. Am J Pathol 1995; 147: 9-19.
2. Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. N Engl J Med 1991; 324: 1-8.
3. Srivastava A, Laidler P, Davies RP, Horgan K, Hughes LE. The prognostic significance of tumor vascularity in intermediate-thickness (0.76-4.0 mm thick) skin melanoma: a quantitative histologic study. Am J Pathol 1988; 133: 419-423.
4. Horak ER, Leek R, Klenk N, et al. Angiogenesis, assessed by platelet/ endothelial cell adhesion molecule antibodies, as indicator of node metastases and survival in breast cancer. Lancet 1992; 340: 1120-1124.
5. Gasparini G, Harris AL. Clinical importance of the determination of tumor angiogenesis in breast carcinoma: much more than a new prognostic tool. J Clin Oncol 1995; 13: 765-782.
6. Barbareschi M, Weidner N, Gasparini G, et al. Microvessel density quantification in breast carcinomas: assessment by light microscopy vs. a computer-aided image analysis system. Appl Immunohist 1995; 3: 75-84.
7. Luthert PJ, Lantos PL. A morphometric study of the microvasculature of a rat glioma. Neuropathol Appl Neurobiol 1985; 11: 461-473.
8. Brawer MK, Deering RE, Brown M, Preston SD, Bigler SA. Predictors of pathologic stage in prostatic carcinoma: the role of neovascularity. Cancer 1994; 73:678-687.
9. Wesseling P, van der Laak JAWM, de Leeuw H, Ruiter DJ, Burger PC. Quantitative immunohistological analysis of the microvasculature in untreated human glioblastoma multiforme: computer-assisted image analysis of whole-tumor sections. J Neurosurg 1994; 81: 902-909.
10. Fox SB, Leek RD, Weekes MP, Whitehouse RM, Gatter KC, Harris AL. Quantitation and prognostic value of breast cancer angiogenesis: comparison of microvessel density, Chalkley count, and computer image analysis. J Pathol 1995; 177: 275-283.
11. Williams JK, Carlson GW, Cohen C, Derose PB, Hunter S, Jurkiewicz MJ. Tumor angiogenesis as a prognostic factor in oral cavity tumors. Am J Surg 1994; 168: 373-380.
12. Charpin C, Devictor B, Bergeret D, et al. CD31 quantitative immunocytochemical assays in breast carcinomas: correlation with current prognostic factors. Am J Clin Pathol 1995; 103: 443-448.
13. Deering RE, Bigler SA, Brown M, Brawer MK. Microvascularity in benign prostatic hyperplasia. Prostate 1995; 26: 111-115.
14. Rucklidge GJ, Travis AJ. An automatic objective estimation of vascularization of normal and tumor-invaded brain tissue using image analysis. Anal Quant Cytol Histol 1989; 11: 286-290.
15. Kohlberger PD, Obermair A, Sliutz G, et al. Quantitative immunohistochemistry of Factor VIII-related antigen in breast carcinoma: a comparison of computer-assisted image analysis with established counting methods. Am J Clin Pathol 1996; 105: 705-710.
16. Goulding H, Abdul Rashid NF, Robertson JF, et al. Assessment of angiogenesis in breast carcinoma: an important factor in prognosis? Hum Pathol 1995; 26: 1196-1200.
17. Smolle J, Soyer HP, Hofmann-Wellenhof R, Smolle-Juettner FM, Kerl H. Vascular architecture of melanocytic skin tumors: a quantitative immunohistochemical study using automated image analysis. Pathol Res Pract 1989; 185: 740-745.
18. Van Muijen GNP, Cornelissen LMHA, Jansen CFJ, et al. Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice. Clin Exp Metastasis 1991; 9: 259-272.
19. Gundersen HJG. Notes on the estimation of the numerical density of arbitrary profiles: the edge effect. J Microsc 1977; 111: 219-223.
20. IBAS User's Manual Release 2.0, 1990.
21. de Jong JS, van Diest PJ, Baak JPA. Heterogeneity and reproducibility of microvessel counts in breast cancer. Lab Invest 1995; 73: 922-926.
22. Hollingsworth HC, Kohn EC, Steinberg SM, Rothenberg ML, Merino MJ. Tumor angiogenesis in advanced stage ovarian carcinoma. Am J Pathol 1995; 147: 33-41.