Comparative PCR: An improved method to detect gene amplification

BioTechniques

Volumn 24, Issue 1, 1998, Pages 22-26

  Brass, Nicole ^a   Heckel, Dirk ^a   Meese, Eckart ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MAGNESIUM CHLORIDE; NUCLEOTIDE;

ARTICLE; CANCER GENETICS; DNA TEMPLATE; GEL ELECTROPHORESIS; GENE; GENE AMPLIFICATION; HUMAN; IN SITU HYBRIDIZATION; ONCOGENE; POLYMERASE CHAIN REACTION; SCINTILLATION COUNTING; SOUTHERN BLOTTING; TUMOR;

EID: 0031887917     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98241bm02     Document Type: Article

References (9)

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3. Pinkel, D., J. Landegent, C. Collins, J. Fuscol, R. Segraves, J. Lucas and J. Gray. 1988. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc. Natl. Acad. Sci. USA 85:9138-9142.
4. Schimke, R.T. 1990. The search for early genetic events in tumorgenesis: an amplification paradigm. Cancer Cells 2:149-151.
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6. Siebert, P.D. and J.W. Larrick. 1992. Competitive PCR. Nature 359:557-558.
7. Slamon, D.J., W. Godolphin, L.A. Jones, J.A. Holt, S.G. Wong, D.E. Keith, W.J. Levin, S.G. Stuart et al. 1989. Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244:707-712.
8. Smith, M.W., S.P. Clark, J.S. Hutchinson, Y.H. Wei, A.C. Churukian, L.B. Daniels, K.L. Diggle, M.W. Gen et al. 1993 A sequence-tagged site map of human chromosome 11. Genomics 17:699-725.
9. Wright, J.A., H.S. Smith, F.M. Watt, M.C. Hancock, D.L. Hudson and G.R. Stark. 1990. DNA amplification is rare in normal human cells. Proc. Natl. Acad. Sci. USA 87:1791-1795.