Detection of CSF leakage by isoelectric focusing on polyacrylamide gel, direct immunofixation of transferrins, and silver staining

Clinical Chemistry

Volumn 44, Issue 2, 1998, Pages 351-353

  Roelandse, Freek W C ^a   Der Van Zwart, Nico ^a   Didden, Jan H ^a   Van Loon, Jenny ^a   Souverijn, John H M ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; POLYACRYLAMIDE GEL; TRANSFERRIN;

ARTICLE; CENTRAL NERVOUS SYSTEM INFECTION; DATA ANALYSIS; HEMOGLOBIN ANALYSIS; ISOELECTRIC FOCUSING; LIQUORRHEA; PROTEIN METABOLISM; REPRODUCIBILITY; RISK FACTOR; TRANSFERRIN BLOOD LEVEL;

EID: 0031887597     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.2.351     Document Type: Article

References (15)

1. Normansell DE, Stacy EK, Booker CF, Butler TZ. Detection of beta-2 transferrin in otorrhea and rhinorrhea in a routine clinical laboratory setting. Clin Diagn Lab Immunol 1994;1:68-70.
2. Meurman OH, Irjala K, Suonpää J, Laurent B. A new method for the identification of cerebrospinal fluid leakage. Acta Otolaryngol 1979;87: 366-9.
3. 2-transferrin in cerebrospinal fluid to detect leakage of cerebrospinal fluid from skull injury. Clin Chem 1992;38:1909-12.
4. Tripathi RC, Millard CB, Tripathi BJ, Noranhe A. Tau fraction of transferrin is present in human aqueous humor and is not unique to cerebrospinal fluid. Exp Eye Res 1990;50:541-7.
5. Skedros DG, Cass SP, Hirsch BE, Kelly RH. Beta-2 transferrin assay in clinical management of cerebral spinal fluid and perilymphatic fluid leaks. J Otolaryngol 1993;22:341-4.
6. Delaroche O, Bordure P, Lippert E, Sagniez M. Perilymph detection by β2-transferrin immunoblotting assay. Application to the diagnosis of perilymphatic fistulae. Clin Chim Acta 1996;245:93-104.
7. Weber PC. Bluestone CD, Kenna MA, Kelly RH. Correlation of beta-2 transferrin and middle ear abnormalities in congenital perilymphatic fistula. Am J Otol 1996;16:277-82.
8. Stibler H. Carbohydrate-deficient transferrin in serum: a new marker of potentially harmful alcohol consumption reviewed. Clin Chem 1991;37: 2029-37.
9. Bell H, Tallaksen C, Sjåheim T, Weberg R, Raknerud N, Ørjasaeter H, et al. Serum carbohydrate-deficient transferrin as a marker of alcohol consumption in patients with chronic liver diseases. Alcohol Clin Exp Res 1993;17: 246-52.
10. Kristiansson B, Andersson M, Tonnby B, Hagberg B. Disialotransferrin development deficiency syndrome. Arch Dis Child 1989;64:71-6.
11. Jaeken J, van Eijk HG, van der Heul C, Corbeel L, Eeckels R, Eggermont E. Sialic acid-deficient serum and cerebrospinal fluid transferrin in a newly recognized genetic syndrome. Clin Chim Acta 1984;144:245-7.
12. Sloman AJ, Kelly RH. Transferrin allelic variants may cause false positives in the detection of cerebrospinal fluid fistulae. Clin Chem 1993;39:1444-5.
13. 2-transferrin-grundlagen und methodik. Laryngol Rhinol Otol (Stuttg) 1986;65:158-61.
14. Reisinger PWM, Hochstrasser K. The diagnosis of CSF fistulae on the basis of detection of beta-2 transferrin by polyacrylamide gel electrophoresis and immunoblotting. J Clin Chem Clin Biochem 1989;27:169-72.
15. Fransen P, Sindic CJM, Thauvoy C, Laterre C, Stroobandt G. Highly sensitive detection of beta-2 transferrin in rhinorrhea and otorrhea as a marker for cerebrospinal fluid leakage. Acta Neurochir (Wien) 1991;109:98-101.