-
1
-
-
0023038088
-
A new way of enhancing the thermostability of proteases
-
Imanaka, T., Shihazaki, M., and Takagi, M.: A new way of enhancing the thermostability of proteases. Nature, 324, 695-697 (1986).
-
(1986)
Nature
, vol.324
, pp. 695-697
-
-
Imanaka, T.1
Shihazaki, M.2
Takagi, M.3
-
2
-
-
0017413189
-
Effect of a single amino acid substitution on stability of conformation of a protein
-
Yutani, K., Ogasawara, K., Sugino, V., and Matsushiro, A.: Effect of a single amino acid substitution on stability of conformation of a protein. Nature, 267, 274-275 (1977).
-
(1977)
Nature
, vol.267
, pp. 274-275
-
-
Yutani, K.1
Ogasawara, K.2
Sugino, V.3
Matsushiro, A.4
-
3
-
-
0023661636
-
Protein engineering of subtilisin BPN': Enhanced stabilization through the introduction of two cysteines to form a disulfide bond
-
Pantoliano, M. W., Ladner, R. C., Bryan, P. N., Rollence, M. L., Wood, J. F., and Poulos, T. L.: Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond. Biochemistry, 26, 2077-2082 (1987).
-
(1987)
Biochemistry
, vol.26
, pp. 2077-2082
-
-
Pantoliano, M.W.1
Ladner, R.C.2
Bryan, P.N.3
Rollence, M.L.4
Wood, J.F.5
Poulos, T.L.6
-
4
-
-
0025232324
-
Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease
-
Takagi, H., Takahashi, T., Momose, H., Inouye, M., Maeda, Y., Matuzawa, H., and Ohta, T.: Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease. J. Biol. Chem., 265, 6874-6878 (1990).
-
(1990)
J. Biol. Chem.
, vol.265
, pp. 6874-6878
-
-
Takagi, H.1
Takahashi, T.2
Momose, H.3
Inouye, M.4
Maeda, Y.5
Matuzawa, H.6
Ohta, T.7
-
5
-
-
0023646665
-
Rational modification of enzyme catalysis by engineering surface change
-
Russell, A. J. and Fersht, A. R.: Rational modification of enzyme catalysis by engineering surface change. Nature, 328, 496-500 (1987).
-
(1987)
Nature
, vol.328
, pp. 496-500
-
-
Russell, A.J.1
Fersht, A.R.2
-
6
-
-
0026548444
-
Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH
-
Sakoda, H. and Imanaka, T.: Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH. J. Bacteriol., 174, 1397-1402 (1992).
-
(1992)
J. Bacteriol.
, vol.174
, pp. 1397-1402
-
-
Sakoda, H.1
Imanaka, T.2
-
7
-
-
0027994512
-
Stabilization and rational design of serine protease AprM under highly alkaline and high-temperature conditions
-
Masui, A., Fujiwara, N., and Imanaka, T.: Stabilization and rational design of serine protease AprM under highly alkaline and high-temperature conditions. Appl. Environ. Microbiol., 60, 3579-3584 (1994).
-
(1994)
Appl. Environ. Microbiol.
, vol.60
, pp. 3579-3584
-
-
Masui, A.1
Fujiwara, N.2
Imanaka, T.3
-
8
-
-
0027281639
-
Purification and properties of the highly thermostable alkaline protease from an alkaliphilic and thermophilic Bacillus sp
-
Fujiwara, N., Masui, A., and Imanaka, T.: Purification and properties of the highly thermostable alkaline protease from an alkaliphilic and thermophilic Bacillus sp. J. Biotechnol., 30, 245-256 (1993).
-
(1993)
J. Biotechnol.
, vol.30
, pp. 245-256
-
-
Fujiwara, N.1
Masui, A.2
Imanaka, T.3
-
9
-
-
0026044870
-
Utilization of a thermostable alkaline protease from an alkalophilic thermophilic for the recovery from used X-ray film
-
Fujiwara, N., Yamamoto, K., and Masui, A.: Utilization of a thermostable alkaline protease from an alkalophilic thermophilic for the recovery from used X-ray film. J. Ferment. Bioeng., 72, 306-308 (1991).
-
(1991)
J. Ferment. Bioeng.
, vol.72
, pp. 306-308
-
-
Fujiwara, N.1
Yamamoto, K.2
Masui, A.3
-
10
-
-
0002396657
-
Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme
-
Fujiwara, N. and Yamamoto, K.: Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme. J. Ferment. Technol., 65, 345-348 (1987).
-
(1987)
J. Ferment. Technol.
, vol.65
, pp. 345-348
-
-
Fujiwara, N.1
Yamamoto, K.2
-
11
-
-
0019449397
-
Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids
-
Imanaka, T., Fujii, M., and Aiba, S.: Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. J. Bacteriol., 146, 1091-1097 (1981).
-
(1981)
J. Bacteriol.
, vol.146
, pp. 1091-1097
-
-
Imanaka, T.1
Fujii, M.2
Aiba, S.3
-
12
-
-
0019838194
-
Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis
-
Imanaka, T., Tanaka, T., Tsunekawa, H., and Aiba, S.: Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis. J. Bacteriol., 147, 776-786 (1981).
-
(1981)
J. Bacteriol.
, vol.147
, pp. 776-786
-
-
Imanaka, T.1
Tanaka, T.2
Tsunekawa, H.3
Aiba, S.4
-
13
-
-
0023813060
-
Cloning and sequence of highly thermostable neutral protease gene from Bacillus stearothermophilus
-
Kubo, M. and Imanaka, T.: Cloning and sequence of highly thermostable neutral protease gene from Bacillus stearothermophilus. J. Gen. Microbiol., 134, 1883-1892 (1988).
-
(1988)
J. Gen. Microbiol.
, vol.134
, pp. 1883-1892
-
-
Kubo, M.1
Imanaka, T.2
-
14
-
-
0040768065
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
-
Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1982).
-
(1982)
Molecular Cloning: A Laboratory Manual
-
-
Maniatis, T.1
Fritsch, E.F.2
Sambrook, J.3
-
15
-
-
0016302499
-
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid
-
Lederberg, E. M. and Cohen, S. N.: Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J. Bacteriol., 119, 1072-1074 (1974).
-
(1974)
J. Bacteriol.
, vol.119
, pp. 1072-1074
-
-
Lederberg, E.M.1
Cohen, S.N.2
-
16
-
-
0001134202
-
Requirements for transformation in Bacillus subtilis
-
Anagnostopoulos, C. and Spizizen, J.: Requirements for transformation in Bacillus subtilis. J. Bacteriol., 81, 741-746 (1961).
-
(1961)
J. Bacteriol.
, vol.81
, pp. 741-746
-
-
Anagnostopoulos, C.1
Spizizen, J.2
-
17
-
-
0020645043
-
New M13 vectors for cloning
-
Messing, J.: New M13 vectors for cloning. Methods Enzymol., 101, 20-78 (1983).
-
(1983)
Methods Enzymol.
, vol.101
, pp. 20-78
-
-
Messing, J.1
-
18
-
-
0023684064
-
A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interaction
-
Higuchi, R., Krummel, B., and Saiki, R. K.: A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interaction. Nucl. Acids Res., 16, 7351-7367 (1988).
-
(1988)
Nucl. Acids Res.
, vol.16
, pp. 7351-7367
-
-
Higuchi, R.1
Krummel, B.2
Saiki, R.K.3
-
19
-
-
0022186670
-
Measurement of protein using bicinchoninic acid
-
Smith, P. K., Krohn, I. R., Hermanson, T. G., Mallia, K. A., Gartner, H. F., Provenzano, D. M., Fujimolo, K. E., Goeke, M. N., Olson, J. B., and Klenk, C. D.: Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85 (1985).
-
(1985)
Anal. Biochem.
, vol.150
, pp. 76-85
-
-
Smith, P.K.1
Krohn, I.R.2
Hermanson, T.G.3
Mallia, K.A.4
Gartner, H.F.5
Provenzano, D.M.6
Fujimolo, K.E.7
Goeke, M.N.8
Olson, J.B.9
Klenk, C.D.10
-
20
-
-
0344534686
-
Role of the pre-pro-region of neutral protease in secretion in Bacillus subtilis
-
Takagi, M. and Imanaka, T.: Role of the pre-pro-region of neutral protease in secretion in Bacillus subtilis. J. Ferment. Bioeng., 67, 71-76 (1989).
-
(1989)
J. Ferment. Bioeng.
, vol.67
, pp. 71-76
-
-
Takagi, M.1
Imanaka, T.2
-
21
-
-
0023156843
-
Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis
-
Amory, A., Kunst, F., Aubert, E., Klier, A., and Rapoport, G.: Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J. Bacteriol., 169, 324-333 (1987).
-
(1987)
J. Bacteriol.
, vol.169
, pp. 324-333
-
-
Amory, A.1
Kunst, F.2
Aubert, E.3
Klier, A.4
Rapoport, G.5
-
22
-
-
84954946520
-
Production of alkaline enzymes by alkalophilic microorganisms. I. Alkaline protease produced by Bacillus no. 221
-
Horikoshi, K.: Production of alkaline enzymes by alkalophilic microorganisms. I. Alkaline protease produced by Bacillus no. 221. Agric. Biol. Chem., 35, 1407-1414 (1971).
-
(1971)
Agric. Biol. Chem.
, vol.35
, pp. 1407-1414
-
-
Horikoshi, K.1
-
23
-
-
0020384747
-
Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis
-
Moran, C. P., Jr., Lang, N., LeGrice, S. F. J., Lee, G., Stephens, M., Sonenshein, A. L., Pero, J., and Losick, R.: Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol. Gen. Genet., 186, 339-346 (1982).
-
(1982)
Mol. Gen. Genet.
, vol.186
, pp. 339-346
-
-
Moran C.P., Jr.1
Lang, N.2
LeGrice, S.F.J.3
Lee, G.4
Stephens, M.5
Sonenshein, A.L.6
Pero, J.7
Losick, R.8
-
24
-
-
0024337616
-
Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain
-
Kaneko, R., Koyama, N., Tsai, Y.-C., Juang, R.-Y., Yoda, K., and Yamasaki, M.: Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain. J. Bacteriol., 171, 5232-5236 (1989).
-
(1989)
J. Bacteriol.
, vol.171
, pp. 5232-5236
-
-
Kaneko, R.1
Koyama, N.2
Tsai, Y.C.3
Juang, R.Y.4
Yoda, K.5
Yamasaki, M.6
-
25
-
-
0021112782
-
Cloning, sequencing and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis
-
Wells, A., Ferrari, E., Henner, D. J., Estell, D. A., and Chen, E. Y.: Cloning, sequencing and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucl. Acids Res., 11, 7911-7925 (1983).
-
(1983)
Nucl. Acids Res.
, vol.11
, pp. 7911-7925
-
-
Wells, A.1
Ferrari, E.2
Henner, D.J.3
Estell, D.A.4
Chen, E.Y.5
-
26
-
-
0021337529
-
Replacement of Bacillus subtilis subtilisin structural gene with an in vitro derived deletion mutation
-
Stahl, M. L. and Ferrari, E.: Replacement of Bacillus subtilis subtilisin structural gene with an in vitro derived deletion mutation. J. Bacteriol., 158, 411-418 (1984).
-
(1984)
J. Bacteriol.
, vol.158
, pp. 411-418
-
-
Stahl, M.L.1
Ferrari, E.2
-
27
-
-
0022429781
-
Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis
-
Jacobs, M., Eliasson, M., Uhlen, M., and Flock, J.-I.: Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucl. Acids Res., 13, 8913-8927 (1985).
-
(1985)
Nucl. Acids Res.
, vol.13
, pp. 8913-8927
-
-
Jacobs, M.1
Eliasson, M.2
Uhlen, M.3
Flock, J.I.4
-
28
-
-
0025731181
-
Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene
-
van der Laan, J. C., Gerritse, G., Mulleners, L. J. S. M., van der Hock, R. A. C., and Quax, W. J.: Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene. Appl. Environ. Microbiol., 57, 901-909 (1991).
-
(1991)
Appl. Environ. Microbiol.
, vol.57
, pp. 901-909
-
-
Van der Laan, J.C.1
Gerritse, G.2
Mulleners, L.J.S.M.3
Van der Hock, R.A.C.4
Quax, W.J.5
-
29
-
-
0022445901
-
Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering
-
Estell, D. A., Graycar, T. P., Miller, J. V., Powers, D. B., Burnier, J. P., Ng, P. G., and Wells, J. A.: Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering. Science, 233, 659-663 (1986).
-
(1986)
Science
, vol.233
, pp. 659-663
-
-
Estell, D.A.1
Graycar, T.P.2
Miller, J.V.3
Powers, D.B.4
Burnier, J.P.5
Ng, P.G.6
Wells, J.A.7
-
30
-
-
0024101334
-
The refined crystal structure of subtilisin Carlsberg at 2.5 Å
-
Neidhart, D. J. and Petsko, G. A.: The refined crystal structure of subtilisin Carlsberg at 2.5 Å. Protein Eng., 2, 271-276 (1988).
-
(1988)
Protein Eng.
, vol.2
, pp. 271-276
-
-
Neidhart, D.J.1
Petsko, G.A.2
-
31
-
-
0026728830
-
Crystal structure of the high-alkaline serine protease PB92 from Bacillus alcalophilus
-
van der Laan, J. M., Teplyakov, A. V., Kelders, H., Kalk, K. H., Misset, O., Mulleners, L. J. S. M., and Dijkstra, B. W.: Crystal structure of the high-alkaline serine protease PB92 from Bacillus alcalophilus. Protein Eng., 5, 405-411 (1992).
-
(1992)
Protein Eng.
, vol.5
, pp. 405-411
-
-
Van der Laan, J.M.1
Teplyakov, A.V.2
Kelders, H.3
Kalk, K.H.4
Misset, O.5
Mulleners, L.J.S.M.6
Dijkstra, B.W.7
-
32
-
-
0014682668
-
Structure of subtilisin BPN' at 2.5 Å resolution
-
Wright, C. S., Alden, R. A., and Kraut, J.: Structure of subtilisin BPN' at 2.5 Å resolution. Nature, 221, 235-242 (1969).
-
(1969)
Nature
, vol.221
, pp. 235-242
-
-
Wright, C.S.1
Alden, R.A.2
Kraut, J.3
-
33
-
-
0019461209
-
Sequence homology in the amino-terminal and active-site regions of thermolabile glyceraldehyde-3-phosphate dehydrogenase from a thermophile
-
Crabb, J. W., Murdock, A. L., Suzuki, T., Hamilton, J. W., McLinden, J. H., and Amelunxen, R. E.: Sequence homology in the amino-terminal and active-site regions of thermolabile glyceraldehyde-3-phosphate dehydrogenase from a thermophile. J. Bacteriol., 145, 503-512(1981).
-
(1981)
J. Bacteriol.
, vol.145
, pp. 503-512
-
-
Crabb, J.W.1
Murdock, A.L.2
Suzuki, T.3
Hamilton, J.W.4
McLinden, J.H.5
Amelunxen, R.E.6
-
34
-
-
0026570383
-
Design of temperature-sensitive penicillinase repressors by replacement of Pro in predicted β-turn structures
-
Imanaka, T., Nakae, M., Ohta, T., and Takagi, M.: Design of temperature-sensitive penicillinase repressors by replacement of Pro in predicted β-turn structures. J. Bacteriol., 174, 1423-1425 (1992).
-
(1992)
J. Bacteriol.
, vol.174
, pp. 1423-1425
-
-
Imanaka, T.1
Nakae, M.2
Ohta, T.3
Takagi, M.4
-
35
-
-
0023430560
-
Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding
-
Matthews, B. W., Nicholson, H., and Backtel, W. J.: Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. Proc. Natl. Acad. Sci. USA, 84, 6663-6667 (1987).
-
(1987)
Proc. Natl. Acad. Sci. USA
, vol.84
, pp. 6663-6667
-
-
Matthews, B.W.1
Nicholson, H.2
Backtel, W.J.3
-
36
-
-
0021193861
-
Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases
-
Kawamura, F. and Doi, R. H.: Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J. Bacteriol., 160, 442-444 (1984).
-
(1984)
J. Bacteriol.
, vol.160
, pp. 442-444
-
-
Kawamura, F.1
Doi, R.H.2
-
37
-
-
0003903343
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
-
Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989).
-
(1989)
Molecular Cloning: A Laboratory Manual, 2nd Ed.
-
-
Sambrook, J.1
Fritsch, E.F.2
Maniatis, T.3
-
38
-
-
0021836559
-
2-terminal region of the penicillinase gene from Bacillus licheniformis on the mode of expression in Bacillus subtilis
-
2-terminal region of the penicillinase gene from Bacillus licheniformis on the mode of expression in Bacillus subtilis. J. Gen. Microbiol., 131, 1753-1763 (1985).
-
(1985)
J. Gen. Microbiol.
, vol.131
, pp. 1753-1763
-
-
Imanaka, T.1
Himeno, T.2
Aiba, S.3
-
39
-
-
0021943518
-
Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mpl8 and pUC18 vectors
-
Yanisch-Perron, C., Vieira, J., and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC18 vectors. Gene, 33, 103-119 (1985).
-
(1985)
Gene
, vol.33
, pp. 103-119
-
-
Yanisch-Perron, C.1
Vieira, J.2
Messing, J.3
|