Fast purification of thioredoxin reductases and of thioredoxins with an unusual redox-active centre from anaerobic, amino-acid-utilizing bacteria

Microbiology

Volumn 144, Issue 3, 1998, Pages 793-800

  Harms, Claudia ^a   Meyer, Manfred A ^a   Andreesen, Jan R ^b  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Amino acid utilizing bacteria; Anaerobic; Thioredoxin; Thioredoxin reductase

Indexed keywords

2 DIETHYLAMINOETHANOL; SEPHAROSE; THIOREDOXIN; THIOREDOXIN REDUCTASE;

AMINO ACID TRANSPORT; ANAEROBIC BACTERIUM; ARTICLE; CLOSTRIDIUM; CONSENSUS SEQUENCE; ELECTRON TRANSPORT; ENZYME PURIFICATION; EUBACTERIUM; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM CYLINDROSPORUM; CLOSTRIDIUM LITORALE; CLOSTRIDIUM SPOROGENES; CLOSTRIDIUM STICKLANDII; ESCHERICHIA COLI; EUBACTERIUM ACIDAMINOPHILUM; TISSIERELLA CREATINOPHILA;

EID: 0031883517     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-144-3-793     Document Type: Article

References (40)

1. Andreesen, J. R. (1994a). Acetate via glycine: a different form of acetogenesis. In Acetogenesis, pp. 568-629. Edited by H. L. Drake. New York/London: Chapman & Hall.
2. Andreesen, J. R. (1994b). Glycine metabolism in anaerobes. Antonie Leeuwenhoek 66, 223-237.
3. Arkowitz, R. A. & Abeles, R. H. (1991). Mechanism of action of clostridial glycine reductase: isolation and characterization of a covalent acetyl enzyme intermediate. Biochemistry 30, 4090-4097.
4. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72, 248-254.
5. Buchanan, B. B., Schurmann, P., Decottignies, P. & Lozano, R. M. (1994). Perspectives in biochemistry and biophysics. Thioredoxin: a multifunctional regulatory protein with a bright future in technology and medicine. Arch Biochem Biophys 314, 257-260.
6. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. (1994). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812-826.
7. Dietrichs, D. & Andreesen, J. R. (1990). Purification and comparative studies of dihydrolipoamide dehydrogenases from anaerobic, glycine-utilizing bacteria Peptostreptococcus glycinophilus, Clostridium cylindrosporum, and Clostridium sporogenes. J Bacteriol 172, 243-251.
8. Dietrichs, D., Meyer, M., Schmidt, B. & Andreesen, J. R. (1990). Purification of NADPH-dependent electron-transferring flavoproteins and N-terminal protein sequence data of dihydrolipoamide dehydrogenases from anaerobic, glycine-utilizing bacteria. J Bacteriol 172, 2088-2095.
9. A and the thioredoxin system, components of the NADPH-dependent reduction of glycine in Eubacterium acidaminophilum and Clostridium litorale. J Bacteriol 173, 5983-5991.
10. Dürre, P. & Andreesen, J. R. (1983). Purine and glycine metabolism by purinolytic clostridia. J Bacteriol 154, 192-195.
11. Eklund, H., Gleason, F. K. & Holmgren, A. (1991). Structural and functional relations among thioredoxins of different species. Protein Struct Funct Genet 11, 13-28.
12. Elliot, J. & Brewer, J. M. (1978). The inactivation of the yeast enolase by 2,3-butanedione. Arch Biochem Biophys 190, 351-357.
13. Follmann, H. & Haeberlein, I. (1995/96).Thioredoxins: universal, yet specific thiol-disulfide redox cofactors. BioFactors 5, 147-156.
14. Freudenberg, W., Dietrichs, D., Lebertz, H. & Andreesen, J. R. (1989). Isolation of an atypically small lipoamide dehydrogenase involved in the glycine decarboxylase complex from Eubacterium acidaminophilum. J Bacteriol 171, 1346-1354.
15. Gleason, F. K. & Holmgren, A. (1988). Thioredoxin and related proteins in prokaryotes. FEMS Microbiol Lett 54, 271-298.
16. Hammel, K. E. & Buchanan, B. B. (1981). Thioredoxin and ferredoxin-thioredoxin reductase activity occur in a fermentative bacterium. FEBS Lett 130, 88-92.
17. Harms, C. (1995). Anaerober Kreatin-Stoffwechsel in Tissierella creatinophila: Anreicherung und Charakterisierung beteiligter Proteine, besonders von verschiedenen Komponenten der Sarkosin-Reduktase. PhD thesis, Universität Göttingen.
18. Holmgren, A. (1968). Thioredoxin. 6. The amino acid sequence of the protein from Escherichia coli B. Eur J Biochem 6, 475-484.
19. Holmgren, A. (1979). Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide. J Biol Chem 254, 9627-9632.
20. Holmgren, A. (1985). Thioredoxin. Annu Rev Biochem 54, 237-271.
21. Holmgren, A. (1989). Thioredoxin and glutaredoxin systems. J Biol Chem 264, 13963-13966.
22. Holmgren, A. & Björnstedt, M. (1995). Thioredoxin and thioredoxin reductase. Methods Enzymol 252, 199-208.
23. Kreimer, S. & Andreesen, J. R. (1995). Glycine reductase of Clostridium litorale. Cloning, sequencing, and molecular analysis of the grdAB operon that contains two in-frame TGA codons for selenium incorporation. Eur J Biochem 234, 192-199.
24. Kreimer, S., Söhling, B. & Andreesen, J. R. (1997). Two closely linked genes encoding thioredoxin and thioredoxin reductase in Clostridium litorale. Arch Microbiol 168, 328-337.
25. Lim, C. J., Fuchs, J. A., McFarlan, S. C. & Hogenkamp, H. P. (1987). Cloning, expression, and nucleotide sequence of a gene encoding a second thioredoxin from Corynebacterium nephridii. J Biol Chem 262, 12114-12119.
26. A. Eur J Biochem 217, 791-798.
27. Meyer, M., Dietrichs, D., Schmidt, B. & Andreesen, J. R. (1991). Thioredoxin elicits a new dihydrolipoamide dehydrogenase with the electron-transferring flavoprotein in Clostridium litorale and Eubacterium acidaminophilum. J Bacteriol 173, 1509-1513.
28. B of betaine reductase and its relationship to the corresponding proteins glycine reductase and sarcosine reductase from Eubacterium acidaminophilum. Eur J Biochem 234, 184-191.
29. Mulrooney, S. B. & Williams, C. H., Jr (1994). Potential active-site base of thioredoxin reductase from Escherichia coli: examination of histidine-245 and aspartate-139 by site-directed mutagenesis. Biochemistry 33, 3148-3154.
30. Naumann, E., Hippe, H. & Gottschalk, G. (1983). Betaine: new oxidant in the Stickland reaction and methanogenesis from betaine and L-alanine by a Clostridium sporogenes-Methanosarcina barkeri coculture. Appl Environ Microbiol 45, 474-483.
31. Perham, R. N., Berry, A. & Scrutton, N. S. (1988). Flavoprotein disulfide oxidoreductases: protein engineering of glutathione reductase from Escherichia cou. Biochem Soc Trans 16, 84-87.
32. Pigiet, V. P. & Conley, R. R. (1977). Purification of thioredoxin, thioredoxin reductase, and glutathione reductase by affinity chromatography. J Biol Chem 252, 6367-6372.
33. Schleicher, A. (1990). Anaerober Abbau von Kreatinin und Kreatin unter Beteiligung von Reduktase-Reaktionen. Diploma thesis, Universität Göttingen.
34. c, a component of glycine reductase from Eubacterium acidaminophilum. Eur J Biochem 206, 79-85.
35. Seto, B. (1980). The Stickland reaction. In Diversity of Bacterial Respiratory Systems, vol. 2, pp. 49-64. Edited by C. J. Knowles. Boca Raton, FL: CRC Press.
36. Stadtman, T. C. (1978). Selenium-dependent clostridial glycine reductase. Methods Enzymol 53, 373-382.
37. Stadtman, T. C. & Davis, J. N. (1991). Glycine reductase protein C. Properties and characterization of its role in the reductive cleavage of Se-carboxymethyl-selenoprotein A. J Biol Chem 266, 22147-22153.
38. Wagner, M. & Andreesen, J. R. (1995). Purification and characterization of threonine dehydrogenase from Clostridium sticklandii. Arch Microbiol 163, 286-290.
39. Williams, C. H. (1992). Lipoamide dehydrogenase, glutathione reductase, thioredoxin reductase, and mercuric ion reductase - a family of flavoenzyme transhydrogenases. In Chemistry and Biochemistry of Flavoenzymes, vol. 3, pp. 121-131. Edited by F. Müller. Boca Raton, FL: CRC Press.
40. 2 or formate. Description and enzymatic studies. Arch Microbiol 150, 254-266.