Polyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: Requirement for 5' conserved sequences

Journal of Virology

Volumn 72, Issue 2, 1998, Pages 1280-1286

  Pritlove, David C ^a   Poon, Leo L M ^a   Fodor, Ervin ^a,b   Sharps, Jane ^a   Brownlee, George G ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; POLYADENYLATED RNA; VIRUS RNA;

ARTICLE; INFLUENZA VIRUS; MESSENGER RNA SYNTHESIS; NONHUMAN; POLYADENYLATION; PRIORITY JOURNAL; VIRION; VIRUS GENE; VIRUS TRANSCRIPTION;

EID: 0031883261     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.2.1280-1286.1998     Document Type: Article

References (27)

1. Cianci, C., L. Tiley, and M. Krystal. 1995. Differential activation of the influenza virus polymerase via template RNA binding. J. Virol. 69:3995-3999.
2. Desselberger, U., V. R. Racaniello, J. J. Zazra, and P. Palese. 1980. The 3′ and 5′ terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8:315-328.
3. Fodor, E., D. C. Pritlove, and G. G. Brownlee. 1994. The influenza virus panhandle is involved in the initiation of transcription. J. Virol. 68:4092-4096.
4. Fodor, E., D. C. Pritlove, and G. G. Brownlee. 1995. Characterization of the RNA-fork model of the virion RNA in the initiation of transcription in influenza A virus. J. Virol. 69:4012-4019.
5. Hagen, M., T. D. Y. Chung, J. A. Butcher, and M. Krystal. 1994. Recombinant influenza virus polymerase: requirement of both 5′ and 3′ viral ends for endonuclease activity. J. Virol. 68:1509-1515.
6. Hay, A. J., and J. J. Skehel. 1979. Characterisation of influenza virus KNA transcripts synthesized in vitro. J. Gen. Virol. 44:599-608.
7. Honda, A., K. Ueda, K. Nagata, and A. Ishihama. 1988. RNA polymerase of influenza virus: role of NP on RNA chain elongation. J. Biochem. 104:1021-1026.
8. Hsu, M., J. D. Parvin, S. Gupta, M. Krystal, and P. Palese. 1987. Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proc. Natl. Acad. Sci. USA 84: 8140-8144.
9. Huang, T. S., P. Palese, and M. Krystal. 1990. Determination of influenza virus proteins required for genome replication. J. Virol. 64:5669-5673.
10. Huang, Z., and J. W. Szostak. 1996. A simple method for 3′-labelling of RNA. Nucleic Acids Res. 24:4360-4361.
11. Kimura, N., M. Nishida, K. Nagata, A. Ishihama, K. Oda, and S. Nakada. 1992. Transcription of a recombinant influenza virus RNA in cells that can express the influenza virus RNA polymerase and nucleoprotein genes. J. Gen. Virol. 73:1321-1328.
12. Lamb, R. F., and R. M. Krug. 1996. Orthomyxoviridae: the viruses and their replication, p. 1353-1395. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Fields virology, 3rd ed. Lippincott-Raven Publishers, Philadelphia, Pa.
13. Li, X., and P. Palese. 1994. Characterization of the polyadenylation signal of influenza virus RNA. J. Virol. 68:1245-1249.
14. Luo, G., W. Luytjes, M. Enami, and P. Palese. 1991. The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure. J. Virol. 65:2861-2867.
15. Luytjes, W., M. Krystal, M. Enami, J. D. Parvin, and P. Palese. 1989. Amplification, expression, and packaging of a foreign gene by influenza virus. Cell 59:1107-1113.
16. Martin, J., C. Albo, J. Ortin, J. A. Melero, and A. Portela. 1992. In vitro reconstitution of active influenza virus ribonucleoprotein complexes using viral proteins purified from infected cells. J. Gen. Virol. 73:1855-1859.
17. Nagata, K., K. Takeuchi, and A. Ishihama. 1989. In vitro synthesis of influenza virus RNA: biochemical complementation assay of factors required for influenza virus replication. J. Biochem. 106:205-208.
18. Parvin, J. D., P. Palese, A. Honda, A. Ishihama, and M. Krystal. 1989. Promoter analysis of the influenza virus RNA polymerase. J. Virol. 63:5142-5152.
19. Perales, B., S. De La Luna, I. Palacios, and J. Ortin. 1996. Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit. J. Virol. 70:1678-1686.
20. Plotch, S. J., and R. M. Krug. 1977. Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA. J. Virol. 21:24-34.
21. Pritlove, D. C. Unpublished data.
22. Pritlove, D. C., E. Fodor, B. L. Seong, and G. G. Brownlee. 1995. In vitro transcription and polymerase binding studies of the termini of influenza virus cRNA: evidence for a cRNA panhandle. J. Gen. Virol. 76:2205-2213.
23. Robertson, J. S. 1979. 5′ and 3′ terminal nucleotide sequences of the RNA genome segments of influenza virus. Nucleic Acids Res. 6:3745-3757.
24. Robertson, J. S., M. Schubert, and R. A. Lazzarini. 1981. Polyadenylation sites for influenza virus mRNA. J. Virol. 38:157-163.
25. Seong, B. L., and G. G. Brownlee. 1992. A new method for reconstituting influenza polymerase and RNA in vitro: a study of the promoter elements for cRNA and vRNA synthesis in vitro and viral rescue in vivo. Virology 186: 247-260.
26. Seong, B. L., M. Kobayashi, K. Nagata, G. G. Brownlee, and A. Ishihama. 1992. Comparison of two reconstitution systems for in vitro transcription and replication of influenza virus. J. Biochem. 111:496-499.
27. Tiley, L. S., M. Hagen, J. T. Matthews, and M. Krystal. 1994 Sequence-specific binding of the influenza virus RNA polymerase to sequences located at the 5′ ends of the viral RNAs. J. Virol. 68:5108-5116.