Detection of HCV and GBV-C/HGV infection by multiplex PCR in plasma samples of transfused subjects

Journal of Virological Methods

Volumn 70, Issue 1, 1998, Pages 79-83

  Caudai, C ^a   Padula, M G ^a   Bettini, V ^a   Valensin, P E ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

Co infection; GBV C HGV; Hepatitis C virus; PCR

Indexed keywords

ARTICLE; BLOOD ANALYSIS; BLOOD TRANSFUSION; CONTROLLED STUDY; HEPATITIS; HEPATITIS C VIRUS; HEPATITIS G VIRUS; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE; VIRUS CHARACTERIZATION;

BLOOD TRANSFUSION; FLAVIVIRIDAE; GENOME, VIRAL; HEPACIVIRUS; HEPATITIS C; HEPATITIS, VIRAL, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TRANSCRIPTION, GENETIC;

EID: 0031881888     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(97)00171-7     Document Type: Article

References (20)

1. Aikawa T., Sugai Y., Okamoto H. Hepatitis G infection in drug abusers with chronic hepatitis C. N. Engl. J. Med. 334:1996;195-196.
2. Alter H.J. The cloning and clinical implications of HGV and HGBV-C. N. Engl. J. Med. 334:1996;1536-1537.
3. Bukh, J., Wantzin, P., Krogsgaard, K., Knudsen, F., Purcell, R.H., 1996. Frequent dual infections of hepatitis C virus (HCV) and recently identified Flaviviridae-like hepatitis agent in dialysis patients. IX Triennal Int. Symp. on Viral Hepatitis and Liver Disease, April 21-25, Rome, Italy. Abstract 253.
4. Chambers T.J., Hahn C.S., Galler R., Rice C.M. Flavivirus genome organization, expression and replication. Annu. Rev. Microbiol. 44:1990;649-688.
5. Chomczysky P., Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate. Phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
6. Choo Q.L., Richman K.H., Han J.H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P.J., Weiner A.J., Bradley D.W., Kuo G., Houghton M. Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA. 88:1991;2451-2455.
7. Fiordalisi G., Zanella I., Mantero G., Bettinardi A., Stellini R., Paraninfo G., Cadeo G., Primi D. High prevalence of GB virus C infection in a group of italian patients with hepatitis of unknown etiology. J. Infect. Dis. 174:1996;181-183.
8. Hadziyannis S., Wages J., Kim J.P. Frequency of viraemia with a new hepatitis virus (HGV) in patients with liver disease and groups at high risk of exposure to blood and blood products. J. Hepatol. 23(1):1995;78.
9. Kwok S., Higuchi R. Avoiding false positive with PCR. Nature. 339:1989;237-238.
10. Leary T.P., Muerhoff A.S., Simons J.N., Pilot-Matias T.J., Erker J.C., Chalmers M.L., Schlauder Dawson G.J., Desai S.M., Mushahwar I.K. Sequence and genomic organzation of GBV-C: A novel member of the Flaviviridae associated with human A-E hepatitis. J. Med. Virol. 48:1996;60-67.
11. Leary T.P., Muerhoff A.S., Simons J.N., Pilot-Matias T.J., Erker J.C., Chalmers M.L., Schlauder Dawson G.J., Desai S.M., Mushahwar I.K. Consensus oligonucleotide primers for the detection of GB virus C in human cryptogenic hepatitis. J. Virol. Methods. 56:1996;119-121.
12. Linnen J., Wages J., Zhang-Keck Z.Y., Fry K.E., Krawczynsky K.Z., Alter H., Koonin E., Gallagher M., Alter M., Hadziyannis S., Karayiannis P., Fung K., Nakatsuji Y., Shih J., Young L., Piatak M., Hoover C., Fernandez J., Chen S., Zou J.-C., Morris T., Hyams K.C., Ismay S., Lifson J.D., Hess G., Foung S.K., Thomas H., Bradley D., Margolis H., Kim J.P. Molecular cloning and disease association of hepatitis G virus: a transfusion-transmissible agent. Science. 271:1996;505-509.
13. Muerhoff A.S., Leary T.P., Simons J.N., Pilot-Matias T.J., Erker J.C., Chalmers M.L., Schlauder G.G., Dawson G.J., Desai S.M., Mushahwar I.K. Genomic organization of GBV-A and GBV-B: two new members of the flaviviridae associated with GB-agent hepatitis. J. Virol. 48:1995;278-283.
14. Nubling C.M., Lower J. GBV-C genomes in a high-risk group, in plasma pools and in intravenous immunoglobulin. Lancet. 347:1996;68.
15. Schlauder G.G., Dawson G.J., Simons J.N., Pilot-Matias T.J., Gutierrez R.A., Heynen C.A., Knigge M.F., Kurpiewski G.S., Buijk S.L., Leary T.P., Murehoff A.S., Desai S.M., Mushahwar I.K. Molecular and serological analysis in the transmission of the GB hepatitis agents. J. Med. Virol. 46:1995;81-90.
16. Schleicher S., Chaves R.L., Dehemer T., Gregor M., Hess G., Flehmig B. Identification of GBV-C hepatitis G RNA in chronic hepatitis patients. J. Med. Virol. 50:1996;71-74.
17. Schlueter V., Schmolke S., Stark K., Hess G., Ofenloch-Haehnle B., Engel A.M. Reverse transcription-PCR detection of hepatitis G virus. J. Clin. Microbiol. 34:1996;2660-2664.
18. Simons J.N., Pilot-Matias T.J., Leary T.P., Dawson G.J., Desai S.M., Shclauder G.G., Muerhoff A.S., Erker J.C., Buijk S.L., Chalmers M.L., Van Sant C.L., Mushahwar I.K. Identification of two flavivirus like genomes in the GB hepatitis agent. Proc. Natl. Acad. Sci. USA. 92:1995;3401-3405.
19. Simons J.N., Leary T.P., Dawson G.J., Pilot-Matias T.J., Muerhoff A.S., Shclauder G.G., Desai S.M., Mushahwar I.K. Isolation of novel virus-like sequences associated with human hepatitis. Nat. Med. 1:1995;564-569.
20. Zuckerman A.J. Alphabet of hepatitis viruses. Lancet. 347:1996;558-559.