A gel electrophoresis study of the competitive effects of monovalent counterion on the extent of divalent counterions binding to DNA

Biophysical Journal

Volumn 74, Issue 2 I, 1998, Pages 964-973

  Li, Anzhi Z ^a   Huang, Haiyan ^a   Re, Xiaodan ^a,b   Qi, Lai J ^a   Marx, Kenneth A ^a  

^a University of Massachusetts Lowell   (United States)

^b Cambridge Finance Partners   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE EARTH METAL; DNA FRAGMENT;

ARTICLE; DNA BINDING; ELECTROPHORETIC MOBILITY; GEL ELECTROPHORESIS; PULSED FIELD GEL ELECTROPHORESIS;

EID: 0031880566     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(98)74019-6     Document Type: Article

References (28)

1. Anderson, C. F., and T. M. Record, Jr. 1982. Polyelectrolyte theories and their applications to DNA. Annu. Rev. Phys. Chem. 33:191-222.
2. Ansorge, W., and L. D. E. Maeyer. 1980. Thermally stabilized very thin polyacrylamide gels for electrophoresis. J. Chromatogr. 202:45-53.
3. 43Ca-NMR is not influenced by terminal phosphate groups at ends and nicks. Biopolymers. 28: 1339-1342.
4. Cantor, C. R., and P. R. Schimmel. 1980. Biophysical Chemistry. Part II. Techniques for the Study of Biological Structure and Function. W. H. Freeman and Co., San Francisco.
5. Daune, M. 1974. Interactions of Metal Ions with Nucleic Acids. Metal Ions in Biological Systems. Marcel Dekker, New York.
6. Duguid, G. J., and V. A. Bloomfield. 1996. Electrostatic effects on the stability of condensed DNA in the presence of divalent cations. Biophys. J. 70:2838-2846.
7. Duguid, G. J., V. A. Bloomfield, J. Benevides, and G. J. Thomas, Jr. 1993. Raman spectroscopy of DNA-metal complexes. I. Interactions and conformational effects of the divalent cations: Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Pd, and Cd. Biophys. J. 65:1916-1928.
8. 2+. Biophys. J. 69:2623-2641.
9. Eichhorn, G. L., and Y. A. Shin. 1968. Interactions of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity. J. Am. Chem. Soc. 90:7323-7328.
10. Granot, J., and D. R. Kearns. 1982. Interactions of DNA with divalent metal ions. III. Extent of metal binding: experiment and theory. Biopolymers. 21:219-232.
11. Grossman, P. D., and D. S. Soane. 1990. Orientation effects on the electrophoretic mobility of rod-shaped molecules in free solution. Anal. Chem. 62:1592-1596.
12. Holzwarth, G., K. J. Platt, C. B. Mckee, R. W. Whitcomb, and G. D. Crater. 1989. The acceleration of linear DNA during pulsed-field gel electrophoresis. Biopolymers. 28:1043-1058.
13. Labarbe, R., S. Flock, C. Maus, and C. Houssier. 1996. Polyelectrolyte counterion condensation theory explains differential scanning calorimetry studies of salt-induced condensation of chicken erythrocyte chromatin. Biochemistry. 35:3319-3327.
14. 2+ ions on calf thymus DNA: binding sites and conformational changes. Biopolymers. 30:743-752.
15. Li, A. Z., H. Huang, and K. A. Marx. 1997. DNA end-to-end distance change due to divalent counterion condensation studied by pulse gel electrophoresis. Statistical Mechanics in Physics and Biology. MRS Symp. Proc. Ser. 463:67-72.
16. Li, A. Z., L. J. Qi, H. H. Shih, and K. A. Marx. 1996. Trivalent counterion condensation on DNA measured by pulse gel electrophoresis. Biopolymers. 38:367-376.
17. Ma, C., and V. A. Bloomfield. 1995. Gel electrophoresis measurement of counterion condensation on DNA. Biopolymers. 35:211-216.
18. Manning, G. S. 1977. Limiting laws and counterion condensation in polyelectrolyte solutions. IV. The approach to the limit and the extraordinary stability of the charge fraction. Biophys. Chem. 7:95-102.
19. Manning, G. S. 1978. The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides. Q. Rev. Biophys. 11:179-246.
20. Manning, G. S., 1981. Limiting laws and counterion condensation in polyelectrolyte solutions. 7. Electrophoretic mobility and conductance. J. Phys. Chem. 85:1508-1515.
21. 2+. Biopolymers. 30:325-333.
22. Perrin, D. D., and B. Dempsey. 1979. Buffers for pH and Metal Ion Control. Chapman and Hall, London.
23. 2+ metal ions on DNA. Master's thesis. Nankai University, Tianjin, China.
24. Rhee, K. W., and B. R. Ware. 1983. DNA-divalent metal cation interactions measured by electrophoretic light scattering. J. Chem. Phys. 78: 3349-3353.
25. Rice, S. A., and M. Nagasawa. 1961. Polyelectrolyte Solutions. Academic Press, New York.
26. Tanford, C. 1967. Physical Chemistry of Macromolecules. John Wiley and Sons, New York.
27. Wilson, R., and V. A. Bloomfield. 1979. Counterion-induced condensation of deoxyribonucleic acid. A light-scattering study. Biochemistry. 18: 2192-2196.
28. Wolfram, S. 1991. Mathematica. A System for Doing Mathematics by Computer. Addison-Wesley Publishing Company, Reading, MA.