Effect of microwave irradiation on antibody labeling efficiency when applied to ultrathin cryosections through fixed biological material

Microscopy Research and Technique

Volumn 42, Issue 1, 1998, Pages 24-32

  Chicoine, Linda ^a   Webster, Paul ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Immunocytochemistry; Labeling efficiency; Microwaves; Quantitation; Ultrathin cryosections

Indexed keywords

ANTIGENS; BIOLOGICAL MEMBRANES; EFFICIENCY; GOLD; IRRADIATION; MICROWAVE IRRADIATION;

ANTIBODY LABELING; GOLD LABELING; IMMUNOCYTOCHEMISTRY; IMMUNOLABELLING; LABELING EFFICIENCIES; LABELINGS; MICROWAVE- IRRADIATIONS; QUANTITATION; ULTRA-THIN; ULTRATHIN CRYOSECTION;

ANTIBODIES;

AMYLASE; ANTIGEN; GOLD; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; MEMBRANE PROTEIN; POLYCLONAL ANTIBODY; PROTEIN A;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; CRYOELECTRON MICROSCOPY; IMMUNOGOLD STAINING; MALE; MICROWAVE IRRADIATION; NONHUMAN; PRIORITY JOURNAL; RAT; TISSUE FIXATION; TISSUE SECTION;

ANIMALIA;

EID: 0031879863     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0029(19980701)42:1<24::AID-JEMT4>3.0.CO;2-R     Document Type: Article

References (26)

1. Amigorena, S., Drake, J.R., Webster, P., and Mellman, I. (1994) Transient accumulation of new class II MHC molecules in a novel endocytic compartment in B lymphocytes. Nature, 369:113-120.
2. Anderson, R.G.W., Falk, J.R., Goldstein, J.L., and Brown, M. (1984) Visualization of acidic organelles in intact cells by electron microscopy. Proc. Natl. Acad. Sci. USA, 81:4838-4842.
3. Chew, E.C., Riches, D.J., Lam, T.K., and Hou Chan, H.J. (1983) A fine structural study of microwave fixation of tissues. Cell Biol. Int. Rep., 7:135-139.
4. Cluett, E.B., and Machamer, C.E. (1996) The envelope of vaccinia virus reveals an unusual phospholipid in Golgi complex membranes. J. Cell Sci., 109:2121-2131.
5. Cuevas, E.C., Bateman, A.C., Wilkins, B.S., Johnson, P.A., Williams, J.H., Lee, A.H.S., Jones, D.B., and Wright, D.H. (1994) Microwave antigen retrieval in immunocytochemistry: A study of 80 antibodies. J. Clin. Pathol., 47:448-452.
6. Giammara, B.L., Birch, D.J., Harper, D.O. (1997) Microwave polymerization of embedding resins for biological/biomedical electron microscopy. Mater. Res. Soc., 189:347-353.
7. Giberson, R.T, and Demaree, J.R.S. (1995) Microwave fixation: Understanding the variables to achieve rapid reproducible results. Microsc. Res. Tech., 32:246-254.
8. Giberson, R.T., Demaree, J.R.S., and Nordhausen, R.W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J. Vet. Diagn. Invest., 9:61-67.
9. Griffiths, G. (1993) Fine Structure Immunocytochemistry. Heidelberg-Berlin, Springer Verlag.
10. Griffiths, G., and Hoppeler, H. (1986) Quantitation in immunocytochemistry: Correlation of immunogold labeling to absolute number of membrane antigens. J. Histochem. Cytochem., 34:1389-1398.
11. Gundersen, H.J.G., Bagger, P., Bendsten, T.F., Evans, S.M., Korbo, L., Marcusen, N., Moller, A., Nielsen, K., Nyengaard, J.R., Pakkenberg, B., Sorensen, F.B., Vesterby, A., and West, M.J. (1988) The new stereological tools: Dissector, fractionator and point-sampled intercepts and their use in pathological research. APMIS, 96:857-881.
12. Hopwood, D., Coghill, G., Ramsay, J., Milne, G., and Kerr, M. (1984) Microwave fixation: Its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy. Histochem. J 16:1171-1191.
13. Leong, A.S., Daymon, M.E., and Milios, J. (1985) Microwave irradiation as a form of fixation for light and electron microscopy. J. Pathol., 146:313-321.
14. Login, G., Stavinoha, W.B., and Dvorak, A.M. (1986) Ultrafast microwave energy fixation for electron microscopy. J. Histochem. Cytochem., 34:381-397.
15. Login, G.R., Galli, S.J., Morgan, E., Arizono, N., Schwartz, L.B., and Dvorak, A.M. (1987a) Rapid microwave fixation of rat mast cells. I. Localization of granule chymase with an ultrastructural postembedding immunogold technique. J. Lab. Invest., 57:592-599.
16. Login, G.R., Schnitt, S.J., and Dvorak, A.M. (1987b) Methods in laboratory investigation: Rapid microwave fixation of human tissues for light microscopy immunoperoxidase identification of diagnostically useful antigens. J. Lab. Invest., 57:585-591.
17. Login, G.R., Galli, S.J., and Dvorak, A.M. (1992) Immunocytochemical localization of histamine in secretory granules of rat peritoneal mast cells with conventional or rapid microwave fixation and an ultrastructural post-embedding immunogold technique. Histochem. Cytochem., 40:1247-1256.
18. Madden, V.J., and Henson, M.M. (1997) Rapid decalcification of temporal bones with preservation of ultrastructure. Hear. Res., 111:76-84.
19. Mayhew, T.M., and Gundersen, H.J.G. (1996) "If you assume, you can make an ass out of u and me," A decade of the dissector for stereological counting of particles in 3D space. J. Anat., 188:1-15.
20. Patterson, M.K.J., and Bulard, R. (1980) Microwave fixation of cells in tissue culture. Stain. Tech., 55:71-75.
21. Rassner, U.A., Crumrine, D.A., Nau, P., and Elias, P.M. (1997) Microwave incubation improves lipolytic enzyme preservation for ultrastructural cytochemistry. Histochem. J., 29:387-392.
22. Shi, S., Key, M.E., and Kalra, K.L. (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem., 39:741-748.
23. Slot, J.W., Posthuma, G., Chang, L.Y., Crapo, J.D., and Geuze, H.J. (1989) Quantitative aspects of immunogold labeling in embedded and in nonembedded sections. Am. J. Anat., 185:271-281.
24. van Deurs, B., Sandvig, K., Petersen, O.W., Olsnes, S., Simons, K., and Griffiths, G. (1988) Estimation of the amount of internalized ricin that reaches the trans-Golgi network. J. Cell Biol., 106:253-267.
25. Webber, M.M., Barnes, F.S., Seltzer, L.A., Bouldin, T.R., and Prasad, K.N. (1980) Short microwave pulses cause ultrastructural membrane damage in neuroblastoma cells. J. Ultrastruct. Res., 71:321-330.
26. Zondervan, P.E., DeJong, A., Sorber, C.W.J., Kok, L.P., DeBruijn, W.C., and VanDerKwast, T.H. (1988) Microwave-stimulated incubation in immunoelectron microscopy: A quantitiative study. Histochem. J., 20:359-364.