Microinjection of Protein Tyrosine Phosphatases into Fibroblasts Disrupts Focal Adhesions and Stress Fibers

Cell Communication and Adhesion

Volumn 5, Issue 3, 1998, Pages 207-219

  Schneider, Galen B ^a,b   Gilmore, Andrew P ^b   Lohse, Daniel L ^c   Romer, Lewis H ^b   Burridge, Keith ^b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

FAK; Focal adhesions; Protein tyrosine phosphatases; Rho; Tyrosine phosphorylation

Indexed keywords

ANTIBODY; FOCAL ADHESION KINASE; PAXILLIN; PROTEIN TYROSINE PHOSPHATASE; RHO FACTOR;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL LYSATE; CONTROLLED STUDY; DEPHOSPHORYLATION; EMBRYO; FIBER; FIBROBLAST CULTURE; IMMUNOFLUORESCENCE MICROSCOPY; MICROINJECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; STRESS;

ANIMALS; CELL ADHESION; CELL ADHESION MOLECULES; CELLS, CULTURED; CYTOSKELETAL PROTEINS; FIBROBLASTS; FOCAL ADHESION KINASE 1; FOCAL ADHESION PROTEIN-TYROSINE KINASES; GTP-BINDING PROTEINS; MICROINJECTIONS; PAXILLIN; PHOSPHOPROTEINS; PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN-TYROSINE KINASES; PROTEIN-TYROSINE-PHOSPHATASE; RATS; RHO GTP-BINDING PROTEINS; STRESS, MECHANICAL; TYROSINE;

ANIMALIA;

EID: 0031877683     PISSN: 10615385     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. Bockholt S. and Burridge K. (1993) Cell spreading on extracellular matrix proteins induces tyrosine phosphorylation of tensin. J. Biol. Chem. 268, 14565-14567.
2. Burridge K. and Chrzanowska-Wodnicka M. (1996) Focal adhesions, contractility and signaling. Annu. Rev. Cell and Dev. Biol. 12, 463-518.
3. Burridge K., Turner C. and Romer L. (1992) Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 4, 893-903.
4. Chong L.D., Traynor-Kaplan A., Bokoch G.M. and Schwartz M.A. (1994) The small GTP-binding protein rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells. Cell. 79, 507-513.
5. Chrzanowska-Wodnicka M. and Burridge K. (1994) Tyrosine phosphorylation is involved in reorganization of the actin cytoskeleton in response to serum or LPA stimulation. J. Cell Sci. 107, 3643-3654.
6. Chrzanowska-Wodnicka M. and Burridge K (1996) Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133, 1403-1415.
7. Clark E.A. and Brugge J.S. (1995) Integrins and signal transduction pathways: the road taken. Science. 14, 233-239.
8. Defilippi P., Bozzo C., Volpe G., Romano G., Ventunno M., Silengo L. and Tarone G. (1994) Integrin-mediated signal transduction in human endothelial cells: analysis of tyrosme phosphorylation events. Cell Adhes. Commun. 2, 75-86.
9. Gilmore A.P. and Burridge K. (1996) Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate. Nature. 381, 531-535.
10. Gilmore A.P. and Romer L.H. (1996) Inhibition of FAK signaling in focal adhesions decreases cell motility and proliferation. Molec. Biol. Cell. 7, 1209-1224.
11. Guan J., Trevithick J. and Hynes R. (1991) Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120 kDa protein. Cell Regul. 2, 951-964.
12. Guan J. L. and Shalloway D. (1992) Regulation of focal adhesion-associated protein tyrosine kinase by cellular adhesion and transformation. Nature (Lond.). 358, 690-692.
13. Guan K.L., Haun R.S., Watson S.J., Geahlen R.L. and Dixon J.E. (1990) Cloning and expression of a protein-tyrosine-phospbatase. Proc. Natl. Acad. Sci. 87, 1501-1505.
14. Haimovich B., Lipfert L., Brugge J.S. and Shattil S.J. (1993) Tyrosine phosphorylation and cytoskeletal reorganization in platelets are triggered by interaction of integrin receptors with their immobilized ligands. J. Biol. Chem. 268, 15868-15877.
15. Hanks S.K., Calalb MB. and Patel S.K (1992) Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment. Proc. Natl. Acad. Sci. USA. 89, 8487-8491.
16. Hildebrand J.D, Schaller M.D. and Parsons J.T. (1993) Identification of sequences required for localization of focal adhesion kinase to focal adhesions. J. Cell Biol. 123, 993-1005.
17. Hynes R (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69, 11-25.
18. Jockusch B.M., Bubeck P., Giehl K., Kroemker M., Moschner J., Rothkegel M., Rudiger M., Schluter K., Stanke G. and Winkler J. (1995) The molecular architecture of focal adhesions Anna. Rev. Cell Dev. Biol. 11, 379-416.
19. Juliano R L. and Haskill S. (1993) Signal transduction from the extracellular matrix. J. Cell Biol. 120, 577-585.
20. Kimura K., Masaaki I., Amano M., Chihara K., Fukata Y., Nakafuku M., Yamamori B., Feng I., Nakano T., Okawa K., Iwamatsu A. and Kaibuchi K. (1996) Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science. 273, 245-248.
21. Komberg L., Earp H.S., Parsons J.T., Schaller M. and Juliano R.L. (1992) Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J. Biol. Chem. 267, 23439-23442.
22. Kornberg L.J., Earp H.S., Turner C.E., Prockop C. and Juliano R.L (1991) Signal transduction by integrins: Increased protein tyrosine phosphorylation caused by clustering of beta 1 integrins. Proc. Natl. Acad. Sci. USA. 88, 8392-8396.
23. Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227, 680-685.
24. Lipfert L, Haimovich B., Schaller M.D., Cobb B.S., Parsons J.T. and Brugge J.S. (1992) Integnn-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets. J. Cell Biol 119, 905-912.
25. Maher P.A., Pasquale E.B., Wang J.Y. and Singer S.J. (1985) Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells. Proc. Natl. Acad. Sci. USA. 82, 6576-6580.
26. Mangeat P. and Burridge K. (1985) Microinjection and immunofluorescence microscopy as tools to study cytoskeletal organization in tissue culture cells. Techniques in Immunocytochemistry. London, Academic Press, Inc. 3: 79-96.
27. McNeil P.L., Murphy R.F., Lanni F. and Taylor D.L. (1984) A method for incorporating macromolecules into adherent cells. J. Cell Biol. 91, 1556-1564.
28. Miyamoto S., Teramoto H., Coso O.A., Gutkind J.S., Burbelo P.D., Akiyama S.K. and Yamada K.M. (1995) Integnn function: molecular hierarchies of cytoskeletal and signaling molecules. J. Cell Biol. 131, 791-805.
29. Nobes C.D., Hawkins P., Stephens L and Hall A. (1995) Activation of the small GTP-binding proteins rho and rac by growth factor receptors. J. Cell Sci. 108, 225-233.
30. Nojima Y., Morino N., Mimura T., Hamasaki K., Furaya H., Sakai R., Sato T., Tachibana K., Morimoto C., Yazaki Y. and Hirai H. (1995) Integrin-mediated cell adhesion promotes tyrosine phosphorylation of p130cas, a src homology 3-containing molecule having multiple src homology 2-binding motifs. J. Biol. Chem. 270, 15398-15402.
31. Pelletier A.J., Bodary S.C. and Levinson A.D. (1992) Signal transduction by the platelet integrin αIIbβ3 : Induction of calcium oscillations required for protein-tyrosine phosphorylation and ligand-induced spreading of stably transfected cells. Molec. Biol. Cell. 3, 989-998.
32. Petch L, Bockholt S., Bouton A., Parsons J.T. and Burridge K. (1995) Adhesion-induced tyrosine phosphorylation of the p130 SRC substrate. J. Cell Sci. 108, 1371-1379.
33. Retta S F., Barry S.T., Critchley D.R., Defilippi P., Silengo L. and Tarone G. (1996) Focal adhesion and stress fiber formation is regulated by tyrosine phosphatase activity. Exp. Cell Res 229, 307-317.
34. Richardson A. and Parsons J.T. (1996) A mechanism for regulation of the adhesion-associated protein tyrosine kinase pp125FAK. Nature. 380, 538-540.
35. Ridley A. (1995) Rho-related proteins: actin cytoskeleton and cell cycle. Curr. Opin. Genet. Dev. 5, 24-30
36. Ridley A.J. and Hall A. (1992) The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell. 70, 389-399.
37. Ridley A.J. and Hall A. (1994) Signal transduction pathways regulating rho-mediated stress fibre formation: requirement for a tyrosine kinase. EMBO. 13, 2600-2610.
38. Romer L.H., McLean N., Turner C. and Burridge K. (1994) Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. Molec. Biol. Cell. 5, 349-361.
39. Schaller M.D., Borgman C.A., Cobb B.S., Vines R.R., Reynolds A.B. and Parsons J.T. (1992) pp125FAK, a structurally unique protein tyrosine kinase associated with focal adhesions. Proc. Natl Arad. Sci. USA. 89, 5192-5196.
40. Schaller M.D., Borgman C.A. and Parsons J.T. (1993) Autonomous expression of a noncatalytic domain of the focal adhesion-associated protein tyrosine kinase pp125FAK Mol. Cell Biol. 13, 785-791.
41. Schwartz M.A., Schaller M.D. and Ginsberg M.H. (1995) INTEGRINS: emerging paradigms of signal transduction. Annu. Rev. Cell Dev. Biol. 11, 549-599.
42. Seufferlein T. and Rozengurt E. (1994) Sphingosine induces pp125FAK and paxillin tyrosine phosphorylation, actin stress fiber formation and focal contact assembly in Swiss 3T3 cells. J. Biol Chem. 269, 27610-27617.
43. Smith D.B. and Johnson K.S. (1988) Single step purification of polypeptides expressed in E. Coli as fusions with glutathione-s-transferase. Gene. 67, 31-40.
44. Takai Y., Sasaki T., Tanaka K. and Nakanishi H. (1995) Rho as a regulator of the cytoskeleton. Trends Biochem. Sci. 20, 227-231.
45. Towbin H., Staehelin T. and Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76, 4350-4354.
46. Vuori K. and Ruoslahti E. (1995) Tyrosine phosphorylation of p130cas and cortactin accompanies integrin-raediated cell adhesion to extracellular matrix. J. Biol. Chem. 270, 22259-22262.
47. Wilson L., Carrier M.J. and Kellie S. (1995) pp125FAK tyrosine kinase activity is not required for the assembly of F-actin stress fibers and focal adhesions in cultured mouse aortic smooth muscle cells. J. Cell Sci. 108, 2381-2391.
48. Zhang Z., Clemens J., Schubert H., Stuckey J., Fischer M., Hume D., Saper M. and Dixon J. (1992) Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase. J. Biol. Chem. 267, 23759-23766.