Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli

Canadian Journal of Microbiology

Volumn 44, Issue 4, 1998, Pages 378-381

  Pelchat, Martin ^a   Lacoste, Lucille ^a   Yang, Fu ^a   Lapointe, Jacques ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Bacillus subtilis; Escherichia coli; Glutamyl tRNA synthetase overproduction; Toxicity

Indexed keywords

GLUTAMATE TRANSFER RNA LIGASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; CYTOTOXICITY; ESCHERICHIA COLI; GENE TRANSFER; GROWTH; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT PLASMID; REGULATOR GENE; SHUTTLE VECTOR;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 0031877001     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjm-44-4-378     Document Type: Article

References (25)

1. Amann, E., Ochs, B., and Abel, K.-J. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene, 69: 301-315.
2. Barker, D. 1982. Cloning and amplified expression of the tyrosyltRNA synthetase genes of Bacillus stearothermophilus and Escherichia coli. Eur. J. Biochem. 125: 357-360.
3. Baron, C., and Böck, A. 1995. The selenocysteine-inserting tRNA species: structure and function. In tRNA: structure, biosynthesis and function. Edited by D. Söll and U. Raj Bhandary. ASM Press, Washington, D.C. pp. 529-544.
4. Brakhage, A.A., Putzer, H., Shazand, K., Röschenthaler, R.J., and Grunberg-Manago, M. 1989. Bacillus subtilis phenylalanyl-tRNA synthetase genes: cloning and expression in Escherichia coli and B. subtilis. J. Bacteriol. 171: 1228-1232.
5. Brand, N.J., and Fersht, A.R. 1986. Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus. Gene, 44: 139-142.
6. Breton, R. 1990. Etude de l'évolution des aminoacyl-tRNA synthétases spécifiques au glutamate et à la glutamine chez Escherichia coli, Bacillus subtilis et Bacillus stearothermophilus. Ph.D. thesis, Laval University, Laval, Que.
7. Breton, R., Watson, D., Yaguchi, M., and Lapointe, J. 1990. Glutamyl-tRNA synthetases of Bacillus subtilis 168T and of Bacillus stearothermophilus. Cloning and sequencing of the gltX genes and comparison with other aminoacyl-tRNA synthetases. J. Biol. Chem. 265: 18 248-18 255.
8. Brückner, R. 1992. A series of shuttle vectors for Bacillus subtilis and Escherichia coli. Gene, 122: 187-192.
9. Gagnon, Y., Breton, R., Putzer, H., Pelchat, M., Grunberg-Manago, M., and Lapointe, J. 1994. Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis. J. Biol. Chem. 269: 7473-7482.
10. Jones, M.D., Lowe, D.M., Borgford, T., and Fersht, A.R. 1986. Natural variation of tyrosyl-tRNA synthetase and comparison with engineered mutants. Biochemistry, 25: 1887-1891.
11. Lapointe, J., and Delcuve, G. 1975. Thermosensitive mutants of Escherichia coll altered in the catalytic subunit and in a regulatory factor of the glutamyl-transfer ribonucleic acid synthetase. J. Bacteriol. 122: 352-358.
12. Gln in vitro. J. Bacteriol. 165: 88-93.
13. Lin, S.X., Brisson, A., Liu, J., Roy, P.H., and Lapointe, J. 1992. Higher specific activity of the Escherichia coli glutamyl-tRNA synthetase purified to homogeneity by a six h procedure. Protein Expression Purif. 3: 71-74.
14. Liu, J., Gagnon, Y., Gauthier, J., Furenlid, L., L'Heureux, P.J., Auger, M., Nureki, O., Yokoyama, S., and Lapointe, J. 1995. The zinc-binding site of Escherichia coli glutamyl-tRNA synthetase is located in the acceptor-binding domain. Studies by extended X-ray absorption fine structure, molecular modeling, and site-directed mutagenesis. J. Biol. Chem. 270: 15 162-15 169.
15. Nureki, O., Vassylyev, D.G., Katayanagi, K., Shimizu, T., Sekine, S., Kigawa, T., Miyazawa, T., Yokoyama, S., and Morikawa, K. 1995. Architectures of class-defining and specific domains of glutamyl-tRNA synthetase. Science (Washington, D.C.), 267: 1958-1965.
16. Proulx, M., and Lapointe, J. 1985. Purification of glutamyl-tRNA synthetase from Bacillus subtilis. Methods Enzymol. 113: 50-54.
17. Proulx, M., Duplain, L., Lacoste, L., Yaguchi, M., and Lapointe, J. 1983. The monomeric glutamyl-tRNA synthetase from Bacillus subtilis 168 and its regulatory factor. Their purification, characterization, and the study of their interaction. J. Biol. Chem. 258: 753-759.
18. Gln and ATP at 2.8 A resolution. Science (Washington, D.C.), 246: 1135-1142.
19. Russell, R.R.B., and Pittard, A.J. 1971. Mutants of Escherichia coli unable to make protein at 42°C. J. Bacteriol. 108: 790-798.
20. Schön, A., Hottinger, H., and Söll, D. 1988. Misaminoacylation and transamidation are required for protein biosynthesis in Lactobacillus bulgarius. Biochimie, 70: 391-394.
21. Stanzel, M., Schön, A., and Sprinzl, M. 1994. Discrimination against misacylated tRNA by chloroplast elongation factor Tu. Eur. J. Biochem. 219: 435-439.
22. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. 1990. Use of T7 RNA polymerase to direct the expression of cloned genes. Methods Enzymol. 185: 60-89.
23. Gln as an intermediate in Bacillus subtilis glutaminyl-tRNA synthesis. Cold Spring Harbor Symp. Quant. Biol. 34: 521-528.
24. Wilcox, M. 1969b. Gamma-glutamyl phosphate attached to glutamine-specific tRNA. A precursor of glutaminyl-tRNA in Bacillus subtilis. Eur. J. Biochem. 11: 405-412.
25. Wilcox, M., and Nirenberg, M. 1968. Transfer RNA as a cofactor coupling amino acid synthesis with that of protein. Proc. Natl. Acad. Sci. U.S.A. 61: 229-236.