Development of a dual color enzyme-linked immunospot assay for simultaneous detection of murine T helper type 1- and T helper type 2-cells

Immunopharmacology

Volumn 39, Issue 2, 1998, Pages 107-116

  Okamoto, Yoshihiro ^a   Abe, Tomoko ^a   Niwa, Takashi ^a   Mizuhashi, Shinobu ^a   Nishida, Mikio ^a  

^a MEIJO UNIVERSITY   (Japan)

Author keywords

Dual color enzyme linked immunospot (ELISPOT) assay; Interleukin 2 (IL 2); Interleukin 4 (IL 4); T helper type 0 (Th0) cell; T helper type 1 (Th1) cell; T helper type 2 (Th2) cell

Indexed keywords

ALKALINE PHOSPHATASE; HORSERADISH PEROXIDASE; INTERLEUKIN 2; INTERLEUKIN 4;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOKINE RELEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; HELPER CELL; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STRAIN DIFFERENCE;

ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; INTERLEUKIN-2; INTERLEUKIN-4; MALE; MICE; MICE, INBRED BALB C; MICE, INBRED C3H; MICE, INBRED C57BL; MICE, INBRED DBA; SENSITIVITY AND SPECIFICITY; TH1 CELLS; TH2 CELLS;

EID: 0031876634     PISSN: 01623109     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0162-3109(98)00007-1     Document Type: Article

References (35)

1. Abbas, A.K., Murphy, K.M., Sher, A., 1996. Functional diversity of helper T lymphocytes. Nature 383, 787-793.
2. + T-cell subsets defined by expression of CD45 isoforms differ in their capac-ity to produce IL-2, IFN-γ and TNF-β. Immunology 81, 253-260.
3. AI-Janadi, M., AI-Balla, S., AI-Dalaan, A., Raziuddin, S., 1993. Cytokine profile in SLE, RA and other rheumatic disease. J. Clin. Immunol. 13, 58-67.
4. Barnard, A., Mahon, B.P., Watkins, J., Redhead, K., Mills, K.H.G., 1996. Th1/Th2 cell dichotomy in acquired immunity to Bordetella pertussis: variables in the in vivo cytokine detection techniques affect the classification of T-cell subsets as Th1, Th2 or Th0. Immunology 87, 372-380.
5. Clerici, M., Hakim, F.T., Venzon, D.J., Blatt, S., Hendrix, C.W., Wynn, T.A., Shearer, G.M., 1993. Changes in IL-2 and IL-4 production in asymptomatic human immunodeficiency virus-seropositive individuals. J. Clin. Invest. 91, 759-765.
6. Corry, D.B., Folkesson, H.G., Warnock, M.L., 1996. Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity. J. Exp. Med. 183, 109-117.
7. Czerkinsky, C., Andersson, G., Ekre, H.-P., Nilsson, L.-A., Klarenskog, L., Ouchterlony, Ö., 1988. Reverse ELISPOT assay for clonal analysis of cytokine production: I. Enumeration of gamma-interferon-secreting cells. J. Immunol. Meth. 110, 29-36.
8. Czerkinsky, C., Nilsson, L.-Å., Nygren, H., Ouchtarony, O., Tarkowski, A., 1983. A solid-phase enzyme-linked immunospot assay for enumeration of specific antibody-secreting cells. J. Immunol. Meth. 65, 109-121.
9. + T cell subset with an unrestricted cytokine profile. J. Immunol. 143, 518-525.
10. Fujihashi, K., McGhee, J.R., Beagley, K.W., McPherson, D.T., McPherson, S.A., Huang, C.-M., Kiyono, H., 1993. Cytokine-specific ELISPOT assay, single cell analysis of IL-2, IL-4 and IL-6 producing cells. J. Immunol. Meth. 160, 181-189.
11. Heinzel, F.P., Sadick, M.D., Holaday, B.J., Coffman, R.L., Locksley, R.M., 1989. Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murin leishmaniasis. Evidence for expression of distinct helper T cell subsets. J. Exp. Med. 169, 59-72.
12. Hutchings, P.R., Cambridge, G., Tite, J.P., Meager, T., Cooke, A., 1989. The detection and enumeration of cytokine-secreting cells in mice and man and the clinical application of these assays. J. Immunol. Meth. 120, 1-8.
13. Krönke, M., Leonard, W.J., Depper, J.M., Arya, S.K., Wong-Staal, F., Gallo, R.C., Waldman, T.A., Greene, W.C., 1984. Cyclosporin A inhibits T-cell growth factor gene expression at the level of mRNA transcription. Proc. Natl. Acad. Sci. USA 81, 5214-5218.
14. Mosmann, T.R., Cherwinski, H., Bond, M.W., Giedlin, M.A., Coffman, R.L., 1986. Two types of murine helper T-cell clone: I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136, 2348-2357.
15. Murray, L.J., Lee, R., Martens, C., 1990. In vitro cytokine gene expression in T cell subsets of the autoimmune MRL/Mp-lpr/lpr mouse. Eur. J. Immunol. 20, 163-170.
16. Noma, Y., Sideras, P., Naito, T., Bergstedt-Lindqvist, S., Azuma, C., Severrinson, E., Tanabe, T., Kinashi, T., Matsuda, F., Yaoita, Y., Honjo, T., 1986. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter. Nature 319, 640-646.
17. Nordström, I., Ferrua, B., 1992. Reverse ELISPOT assay for clonal analysis of cytokine production: II. Enumeration of interleukin-1-secreting cells by amplified (avidin-biotin antiperoxidase) assay. J. Immunol. Meth. 150, 199-206.
18. Okamoto, Y., Murakami, H., Nishida, M., 1997. Detection of interleukin-6 producing cells among the various organs in normal mice with an improved enzyme-linked immunospot (ELISPOT) assay. Endocrine J. 44, 349-355.
19. Parronchi, P., Macchia, D., Piccini, M.-P., Biswas, P., Simonelli, C., Maggi, E., Ricci, M., Ansari, A.A., Romagnani, S., 1991. Allergen and bacterial antigen specific T-cell clones established from atopic donors show a different profile of cytokine production. Proc. Natl. Acad. Sci. USA 88, 4538-4542.
20. Plaut, M., Pierce, J.H., Watoson, C.J., Hanley-Hyde, J., Nordan, R.P., Paul, W.E., 1989. Mast cell lines produce lymphokines in response to cross-linkage of FceRI or to calcium ionophores. Nature 339, 64-67.
21. Pope, M., Chuang, S.W., Mosmann, T., Leibowitz, J.L., Gorczynski, R.M., Levy, G.A., 1996. Resistance of naive mice to murine hepatitis virus strain 3 requires development of Th1, but not a Th2 response, wheres pre-existing antibody partially protects against primary infection. J. Immunol. 156, 3342-3349.
22. Ruccetti, F.W., Morgan, D.A., Garo, R.C., 1977. Functional and morphologic characterization of human T cells continuously grown in vitro. J. Immunol. 119, 131-138.
23. Scott, B., Librau, R., Degerman, S., Marconi, L.A., Ogata, L., Caton, A.J., McDevtt, H.O., Lo, D., 1994. A role for non-MHC genetic polymorphism in susceptibility to spontaneous autoimmunity. Immunity 1, 73-83.
24. + T cell can be primed in vitro to produce IL-4. J. Immunol. 148, 1652-1656.
25. Sedgwick, J.D., Czerkinsky, C., 1992. Detection of cell-surface molecules, secreted products of single cells and cellular proliferation by enzyme immunoassay. J. Immunol. Meth. 150, 159-175.
26. Segal, R., Bermas, B., Dayan, M., Kalush, F., Shearer, G., Mozes, E., 1997. Kinetics of cytokine production in experimental systemic lupus erytematosus; involvement of T helper celll/T helper cell 2-type cytokines in disease. J. Immunol. 158, 3009-3016.
27. Shanker, A.M., Titus, R.G., 1995. T cell and non-T cell compart-ments can independently determine resistance to Leishmania major. J. Exp. Med. 181, 845-855.
28. Shirai, A., Conover, J., Klinman, D.M., 1995. Increased activation and altered ratio of interferon-gamma: interleukin-4 secreting cells in MRL-lpr/lpr mice. Autoimmunity 21, 107-116.
29. Shirai, A., Holmes, K., Klinman, D., 1993. Detection and quantitation of cells secreting IL-6 under physiologic conditions in BALB/c mice. J. Immunol. 150, 793-799.
30. Shirai, A., Sierra, V., Kelly, C.I., Klinman, D.M., 1994. Individual cells simultaneously produce both IL-4 and IL-6 in vivo. Cytokine 6, 329-336.
31. Street, N.B., Schumacher, J.H., Fong, T.A.T., Bass, H., Fiorentino, D.F., Leverah, J.A., Mosmann, T.R., 1990. Heterogeneity of mouse helper T cell: evidence from bulk cultures and limitating dilution cloning for precursors of Th1 and Th2 cells. J. Immunol. 144, 1629-1639.
32. Taguchi, T., McGhee, J.R., Coffman, R.L., Beagley, K.W., Eldridge, J.H., Takatsu, K., Kiyono, H., 1990. Detection of individual mouse splenic T cells producing IFN-g and IL-5 using the enzyme-linked immunospot (ELISPOT) assay. J. Immunol. Meth. 128, 65-73.
33. Tanaka, M., Miyazaki, H., Takeda, Y., Nakamura, T., Takeo, S., 1992. Effect of oral administration of the α-1,6-polysaccharide SPR-901 on suppressed Con A-induced IL-2 production in tumour-bearing mice. Int. J. Immunotherapy 8, 177-184.
34. Tanguay, S., Killion, J.J., 1994. Direct comparison of ELISPOT and ELISA-based assays for detection of individual cytokine-secreting cells. Lymphokine Cytokine Res. 13, 259-263.
35. + T lymphocytes in atopic patients. J. Immunol. 144, 4651-4656.