Using distilled water for the extraction of mucosal antibodies and the subsequent application in RSV neutralization test

Journal of Immunoassay

Volumn 19, Issue 2-3, 1998, Pages 209-222

  Hu, Ke Fei ^a   Morein, Bror ^a,b   Merza, Malik ^b  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

Antibody extraction; ELISA; Immunostimulating complexes (ISCOMs); Respiratory syncytial virus (RSV); Saponin; Virus neutralization (VN) test

Indexed keywords

RESPIRATORY SYNCYTIAL VIRUS VACCINE; WATER;

ANIMAL EXPERIMENT; ANTIBODY SPECIFICITY; ARTICLE; DISTILLATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; IMMUNOASSAY; MOUSE; MUCOSAL IMMUNITY; NONHUMAN; VIRUS NEUTRALIZATION;

EID: 0031872567     PISSN: 01971522     EISSN: None     Source Type: Journal    
DOI: 10.1080/01971529808005481     Document Type: Article

References (6)

1. Murphy, B.R. and Walsh, E.E. Formalin-inactivated respiratory syncytial vaccine induces antibodies to fusion glycoproteins that are deficient in fusion inhibiting activity. J. Clin. Microbiol. 1988; 26: 1595-7.
2. Prince, G.A., Jensen, A.B., Hemming, V.G., et al. Enhancement of respiratory syncytial virus pulmonary pathology in cotton rats by prior intramuscular inoculation of formalin-inactivated virus. J. Virol. 1986; 57: 721-8.
3. Sander, B., Andersson, J. and Andersson, U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol. Rev. 1991; 119: 65-93.
4. Bergquist, C., Lagergård, T., Lindblad, M. and Holmgren, J. Local and systemic antibody responses to dextran-cholera toxin B subunit conjugates. Infect. Immun. 1995; 63: 2021-5.
5. Jennings, R., Smith, T., Potter, C.W. Use of enzyme-linked immunosorbent assay (ELISA) for the estimation of serum antibodies in an influenza virus vaccine study. Med. Microbiol. Immunol. 1981; 169: 247-58.
6. Trudel, M., Nadon, F., Séguin, C., Simard, C. and Lussier, G. Experimental polyvalent ISCOMs subunit vaccine induces antibodies that neutralize human and bovine respiratory syncytial virus. Vaccine 1988; 7: 12-6.