A novel technique for isolation of Frankia and generation of single-spore cultures

Canadian Journal of Microbiology

Volumn 44, Issue 5, 1998, Pages 490-492

  Sarma, Gopa ^a   Sen, Arnab ^a   Varghese, Rajani ^a   Misra, Arvind K ^a  

^a NORTH EASTERN HILL UNIVERSITY   (India)

Author keywords

Alginate beads; Frankia; Isolation; Single spore cultures

Indexed keywords

CALCIUM ALGINATE;

ARTICLE; BACTERIUM ISOLATION; DNA PURIFICATION; FRANKIA; PRIORITY JOURNAL; TECHNIQUE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); FRANKIA; UNCULTURED ACTINOMYCETE;

EID: 0031869256     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjm-44-5-490     Document Type: Article

References (14)

1. Baker, D., and O'Keefe, D. 1984. A modified sucrose fractionation procedure for the isolation of frankiae from actinorhizal root nodules and soil samples. Plant Soil, 78: 23-28.
2. Borthakur, M., Sen, A., and Misra, A.K. 1996. Immobilized Frankia spores remained viable on dry storage and on restoration to medium regenerated active colonies. Plant Soil, 181: 227-231.
3. Borthakur, M., Sen, A., and Misra, A.K. 1997. Exogenous nitrogen sources do not drastically reduce nitrogenase activity in polymer entrapped Frankia. Indian J. Exp. Biol. 35: 173-175.
4. Bosco, M., Fernandez, M.P., Simonet, P., Materassi, R., and Normand, P. 1992. Evidence that some Frankia sp. strains are able to cross boundaries between Alnus and Elaeagnus host specificity groups. Appl. Environ. Microbiol. 58: 1569-1576.
5. Diem, H.G., and Dommergues, Y. 1983. The isolation of Frankia from nodules of Casuarina. Can. J. Bot. 61: 2822-2825.
6. Dobritsa, S.V., and Stupar, O.S. 1989. Genetic heterogeneity among Frankia isolates from root nodules of individual actinorhizal plants. FEMS Microbiol. Lett. 58: 287-292.
7. Ganesh, G. 1993. Study of genetic diversity of Frankia alni strains isolated from Alnus nepalensis root nodules found in Meghalaya. Ph.D. thesis, North-Eastern Hill University, Shillong, India.
8. Ganesh, G., Misra, A.K., Chapelon, C., and Normand, P. 1994. Morphological and molecular characterization of Frankia sp. isolates from root nodules of Alnus nepalensis Don. Arch. Microbiol. 161: 152-155.
9. Lechevalier, M.P., and Lechevalier, H.A. 1990. Systematics, isolation and culture of Frankia. In The biology of Frankia and actinorhizal plants. Edited by C.R. Schwintzer and J.D. Tjepkema. Academic Press, New York, pp. 35-60.
10. Lumini, E., and Bosco, M. 1996. PCR-restriction fragment length polymorphism identification and host range of single spore isolates of flexible Frankia sp. strain UFI132715. Appl. Environ. Microbiol. 62: 3026-3029.
11. Nalin, R., Domenach, A.-M., and Normand, P. 1995. Molecular structure of the Frankia spp. nifD-K intergenic spacer and design of Frankia genus compatible primer. Mol. Ecol. 4: 483-491.
12. Normand, P., Orso, S., Cournoyer, B., Jeannin, P., Chapelon, C., Dawson, J., Evtushenko L., and Misra, A.K. 1996. Molecular phylogeny of the genus Frankia and related genera and emendation of the family Frankiaceae. Int. J. Syst. Bacteriol. 46: 1-9.
13. Prin, Y., Maggia, L., Picard, B., Diem, H.G., and Goullet, P. 1991. Electrophoretic comparison of enzymes from 22 single-spore cultures obtained from Frankia strain ORS140102. FEMS Microbiol. Lett. 77: 223-228.
14. Sen, A. 1996. Electron microscopy and molecular biology of Frankia. Ph.D. thesis, North-Eastern Hill University, Shillong, India.