A fluorescent gram stain for flow cytometry and epifluorescence microscopy

Applied and Environmental Microbiology

Volumn 64, Issue 7, 1998, Pages 2681-2685

  Mason, David J ^a   Shanmuganathan, Subo ^a   Mortimer, Fiona C ^b   Gant, Vanya A ^a  

^a ST THOMAS HOSPITAL   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HEXIDIUM IODIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIUM EXAMINATION; DIAGNOSTIC PROCEDURE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GRAM STAINING; HISTOGRAM; NONHUMAN; PROTEIN ANALYSIS;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA;

EID: 0031867813     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.7.2681-2685.1998     Document Type: Article

References (20)

1. Allman, R., R. Manchee, and D. Lloyd. 1993. Flow cytometric analysis of heterogeneous bacterial populations, p. 27-47. In D. Lloyd (ed.), Flow cytometry in microbiology. Springer-Verlag, London, England.
2. Beveridge, T. J. 1990. Mechanism of Gram variability in select bacteria. J. Bacteriol. 172:1609-1620.
3. Dahlen, G., and I. Mattsby-Baltzer. 1983. Lipid A in anaerobic bacteria. Infect. Immun. 39:466-468.
4. Davey, H. M., and D. B. Kell. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single cell analyses. Microbiol. Rev. 60:641-696.
5. Esteban, J., G. García-Calvo, P. Jiménez-Castillo, and K. Soriano. 1996. Failure of Gram stain to detect Propionibacterium acnes in specimens from clinically significant infections. J. Clin. Microbiol. 34:2051.
6. Gant, V. A., G. Warnes, I. Phillips, and G. F. Savidge. 1993. The application of flow cytometry to the study of bacterial responses to antibiotics. J. Med. Microbiol. 39:147-154.
7. Goodhart, G. L., E. Abrutyn, R. Watson, R. K. Root, and J. Egert. 1977. Community-acquired Acinetobacter calcoaceticus var. anitratus pneumonia. JAMA 238:1516-1518.
8. Gregerson, T. 1978. Rapid method for distinction of gram-negative from gram-positive bacteria. Eur. J. Appl. Microbiol. Biotechnol. 5:123-127.
9. Halebian, S., B. Harris, S. M. Finegold, and R. D. Rolfe. 1981. Rapid method that aids in distinguishing gram-positive from gram-negative anaerobic bacteria. J. Clin. Microbiol. 13:444-448.
10. Harrington, B. J. 1997. Letter. Clin. Microbiol. Newsl. 19:191.
11. Haughland, R. P. 1996. Nucleic acid detection, p. 143-168. In M. T. Z. Spence (ed.), Handbook of fluorescent probes and research chemicals. Molecular Probes, Inc., Eugene, Oreg.
12. Johnson, M. J., E. Thatcher, and M. E. Cox. 1995. Techniques for controlling variability in Gram staining of obligate anaerobes. J. Clin. Microbiol. 33: 755-758.
13. Nikaido, H., and M. Vaara. 1985. Molecular basis of outer membrane permeability. Microbiol. Rev. 49:1-32.
14. Nikaido, H., and M. Vaara. 1987. Outer membrane, p. 7-22. In F. Neidhardt, J. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
15. Olsen, G. J., C. R. Woese, and R. Overbeek. 1994. The winds of (evolutionary) change: breathing new life into microbiology. J. Bacteriol. 176:1-6.
16. Poot, M., L. L. Gibson, and V. L. Singer. 1997. Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry. Cytometry 27:358-364.
17. Roth, B. L., M. Poot, S. T. Yue, and P. J. Millard. 1997. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl. Environ. Microbiol. 63:2421-2431.
18. Shapiro, H. M. 1995. Practical flow cytometry, 3rd ed., p. 367-425. Alan R. Liss, Inc., New York, N.Y.
19. Sizemore, R. K., J. J. Caldwell, and A. S. Kendrick. 1990. Alternate Gram staining technique using a fluorescent lectin. Appl. Environ. Microbiol. 56: 2245-2247.
20. Verghese, A., and S. L. Berk. 1983. Bacterial pneumonia in the elderly. Medicine 62:271-285.