Detection of the SYT-SSX fusion transcripts in formaldehyde-fixed, paraffin-embedded tissue: A reverse transcription polymerase chain reaction amplification assay useful in the diagnosis of synovial sarcoma

Modern Pathology

Volumn 11, Issue 7, 1998, Pages 626-633

  Lasota, Jerzy ^a   Jasinski, Marek ^a   Debiec Rychter, Maria ^b   Szadowska, Anna ^b   Limon, Janusz ^c   Miettinen, Markku ^a  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b MEDICAL UNIVERSITY OF LODZ   (Poland)

^c MEDICAL UNIVERSITY OF GDANSK   (Poland)

Author keywords

Polymerase chain reaction; Synovial sarcoma; SYT SSX fusion transcript

Indexed keywords

ARTICLE; DIAGNOSTIC VALUE; GENE FUSION; GENE SEQUENCE; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYNOVIAL SARCOMA;

ADOLESCENT; ADULT; ANTIGENS, NEOPLASM; BASE SEQUENCE; CA-15-3 ANTIGEN; FEMALE; FROZEN SECTIONS; HUMANS; IMMUNOENZYME TECHNIQUES; KARYOTYPING; KERATINS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; ONCOGENE PROTEINS, FUSION; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; PROTEINS; PROTO-ONCOGENE PROTEINS; REPRESSOR PROTEINS; SARCOMA, SYNOVIAL; TRANSCRIPTION, GENETIC; TRANSLOCATION, GENETIC;

EID: 0031867496     PISSN: 08933952     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Enzinger FM, Weiss SW. Soft tissue tumors. 3rd ed. St. Louis: Mosby; 1995. p. 757-86.
2. Limon J, Dal Cin P, Sandberg AA. Translocations involving the X chromosome in solid tumors: presentation of two sarcomas with t(X;18)(q13;p11). Cancer Genet Cytogenet 1986;23:87-91.
3. Turc-Carel C, Dal Cin P, Limon J, Rao U, Li FP, Corson JM, et al. Involvement of chromosome X in primary cytogenetic change in human neoplasia: nonrandom translocation in synovial sarcoma. Proc Natl Acad Sci U S A 1987;84:1981-5.
4. Limon J, Mrozek K, Mandahl N, Nedoszytko B, Verhest A, Rys J, et al. Cytogenetics of synovial sarcoma: presentation of 10 new cases and review of the literature. Genes Chromosomes Cancer 1991;3:338-45.
5. Dal Cin P, Rao U, Jani-Sait S, Karakousis C, Sandberg AA. Chromosome in the diagnosis of soft tissue tumors. I. Synovial sarcoma. Mod Pathol 1992;5:57-62.
6. Mandahl N, Limon J, Mertens F, Nedoszytko B, Gibas Z, Denis A, et al. Nonrandom secondary chromosome aberrations in synovial sarcomas with t(X;18). Int J Oncol 1995;7: 495-9.
7. Szadowska A, Olborski B, Nedoszytko B, Limon J. Usefulness of cytogenetic analysis in the diagnosis of undifferentiated synovial sarcoma of the chest wall: a case report. Pol J Pathol 1995;46:199-202.
8. Clark J, Rocques PJ, Crew AJ, Gill S, Shipley J, Chan AM-L, et al. Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma. Nat Genet 1994;7:502-8.
9. de Leeuw B, Balemans M, Olde Weghuis D, Geurts van Kessel A. Identification of two alternative fusion genes, SYT-SSX1 and SYT-SSX2, in t(X;18)(p11.2;q11.2)-positive synovial sarcomas. Hum Mol Genet 1995;4:1097-9.
10. Crew J, Clark J, Fisher C, Gill S, Grimer R, Chand A, et al. Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Krüppel-associated box in human synovial sarcoma. EMBO J 1995;14:2333-40.
11. Fligman I, Lonardo F, Jhanwar SC, Gerald WL, Woodruff J, Ladanyi M. Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript. Am J Pathol 1995;147:1592-9.
12. de Leeuw B, Balemans M, Geurts van Kessel A. A novel Krüppel-associated box containing the SSX gene (SSX3) on the human X chromosome is not implicated in t(X;18)-positive synovial sarcomas. Cytogenet Cell Genet 1996;73:179-83.
13. Gure AO, Türeci Ö, Sahin U, Tsang S, Scanlan MJ, Jäger E, et al. SSX: a multigene family with several members transcribed in normal testis and human cancer. Int J Cancer 1997;72:965-71.
14. Mitelman F, editor. An international system for human cytogenetic nomenclature. Basel: Karger; 1995.
15. Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol 1990;43:499-504.
16. Ladanyi M. The emerging molecular genetics of sarcoma translocations. Diagn Mol Pathol 1995;4:162-73.
17. Barr FG, Chatten J, D'Cruz CM, Wilson AE, Nauta LE, Nycum LM, et al. Molecular assays for chromosomal translocations in the diagnosis of pediatric soft tissue sarcomas. JAMA 1995;273:553-7.
18. Downing JR, Khandekar A, Shurtleff SA, Head DR, Parham DM, Webber BL, et al. Multiplex RT-PCR assay for the differential diagnosis of alveolar rhabdomyosarcoma and Ewing's sarcoma. Am J Pathol 1995;146:626-34.
19. Argani P, Zakowski MF, Klimstra DS, Rosai J, Ladanyi M. Detection of the SYT-SSX chimeric RNA of synovial sarcoma in paraffin-embedded tissue and its application in problematic cases. Mod Pathol 1997;11:65-71.
20. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-8.
21. Renwick PJ, Reeves BR, Dal Cin P, Fletcher CDM, Kempski H, Sciot R, et al. Two categories of synovial sarcoma defined by divergent chromosome translocation breakpoints in Xp11.2, with implications for the histologic subclassification of synovial sarcoma. Cytogenet Cell Genet 1995;70:58-63.
22. de Leeuw B, Suijkerbujik, RF, Olde Weghuis D, Meloni AM, Stenman G, Kindblom GL, et al. Distinct Xp11.2 breakpoint regions in synovial sarcoma revealed by metaphase and interphase FISH: relationship to histologic subtypes. Cancer Genet Cytogenet 1994;73:89-94.
23. Janz M, de Leeuw B, Olde Weghuis D, Werner M, Nolte M, Geurts van Kessel A, et al. Interphase cytogenetic analysis of distinct X-chromosomal translocation breakpoints in synovial sarcoma. J Pathol 1995;175:391-6.
24. Kawai A, Woodruff J, Healey JH, Brennan MF, Antonescu CR, Ladanyi M. SYT-SSX gene fusion as a determinant of morphology and prognosis in synovial sarcoma. N Engl J Med 1998;338:153-60.
25. Kashima T, Matsushita H, Kuroda M, Takeuchi H, Udagawa H, Ishida T, et al. Biphasic synovial sarcoma of the peritoneal cavity with t(X;18) demonstrated by reverse transcriptase polymerase chain reaction. Pathol Int 1997;47:637-41.
26. Nagao K, Ito H, Yoshida H. Chromosomal translocation t(X; 18) in human synovial sarcomas analyzed by fluorescence in situ hybridization using paraffin-embedded tissue. Am J Pathol 1996;148:601-9.
27. Shipley J, Crew J, Birdsall S, Gill S, Clark J, Fisher C, et al. Interphase fluorescence in situ hybridization and reverse transcription polymerase chain reaction as a diagnostic aid for synovial sarcoma. Am J Pathol 1996;148:559-67.
28. Lee W, Han K, Harris CHP, Shim S, Kim S, Meisner L. Use of FISH to detect chromosomal translocations and deletions. Am J Pathol 1993;143:15-9.
29. Poteat HT, Corson JM, Fletcher JA. Detection of chromosome 18 rearrangement in synovial sarcoma by fluorescence in situ hybridization. Cancer Genet Cytogenet 1995;84:76-81.