Cyclopentyladenosine-induced homologous down-regulation of A1 adenosine receptors (A1AR) in intact neurons is accompanied by receptor sequestration but not a reduction in A1AR mRNA expression or G protein α-subunit content

Journal of Neurochemistry

Volumn 71, Issue 1, 1998, Pages 221-230

  Hettinger, Barbara D ^a   Leid, Mark ^a   Murray, Thomas F ^b  

^a OREGON STATE UNIVERSITY   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

Adenosine; G proteins; Homologous down regulation; mRNA stability; Neurons; Sequestration

Indexed keywords

ADENOSINE A1 RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; MESSENGER RNA; PROTEIN SUBUNIT;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME INHIBITION; GRANULE CELL; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RNA ANALYSIS; RNA TRANSCRIPTION;

EID: 0031866370     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1998.71010221.x     Document Type: Article

References (40)

1. 3 muscarinic receptor subtypes on cerebellar granule cells in primary culture. J. Neurochem. 55, 334-337.
2. 1 adenosine receptors. Mol. Pharmacol. 50, 104-111.
3. Blair T. A., Parenti M., and Murray T. F. (1989) Development of pharmacological sensitivity to adenosine analogs in embryonic chick heart: role of A, adenosine receptors and adenylyl cyclase inhibition. Mol. Pharmacol. 35, 661-670.
4. Böhm S. K., Grady E. F., and Bunnett N. W. (1997) Regulatory mechanisms that modulate signalling by G-protein-coupled receptors. Biochem. J. 322, 1-18.
5. Brann M. R., Collins R. M., and Spiegel A. (1987) Localization of mRNAs encoding the α-subunits of signal-transducing G-proteins within rat brain and among peripheral tissues. FEBS Lett. 222, 191-198.
6. 2-adrenergic receptor gene in response to short-term agonist exposure. Proc. Natl. Acad. Sci. USA 86, 4853-4857.
7. Daly J. W. (1982) Adenosine receptors: targets for future drugs. J. Med. Chem. 25, 197-207.
8. 1-adenosine receptor system in the guinea pig atrioventricular node. J. Pharmacol. Exp. Ther. 272, 1024-1035.
9. 1 receptors inhibit adenylate cyclase activity and neurotransmitter release and hyperpolarize pyramidal neurons in rat hippocampus. J. Pharmacol. Exp. Ther. 249, 31-37.
10. 2C2. J. Biol. Chem. 267, 25473-25479.
11. Fernandez M., Svenningsson P., and Fredholm B. B. (1995) Adaptive changes in adenosine receptors following long-term treatment with the adenosine receptor agonist R-phenylisopropyl-adenosine. Life Sci. 58, 769-776.
12. 3-muscarinic acetylcholine receptor mRNA and protein in cultured cerebellar granule cells. Mol. Pharmacol. 44, 940-949.
13. Gilman M. (1992) Ribonuclease protection assay, in Short Protocols in Molecular Biology, 2nd edit. (Ausubel F.M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., and Struhl K. eds), pp. 4-18-4-21. John Wiley and Sons, New York.
14. 2-adrenergic receptor. Nature 383, 447-450.
15. 3H]-cyclohexyladenosine. J. Neurosci. 2, 1230-1241.
16. 1 adenosine receptor agonist. J. Biol. Chem. 265, 5206-5210.
17. i down regulation as a mechanism for heterologous desensitization in adipocytes. J. Biol. Chem. 276, 3223-3229.
18. 1 adenosine receptor-adenylyl cyclase system in cerebellar granule cells. J. Neurochem. 67, 1921-1930.
19. Hoffman B. B., Chang H., Dall' Aglio E., and Reaven G. M. (1986) Desensitization of adenosine receptor-mediated inhibition of lipolysis: the mechanism involves the development of enhanced cyclic adenosine monophosphate accumulation in tolerant adipocytes. J. Clin. Invest. 78, 185 - 190.
20. 6 glioma cells: effects of agonist, forskolin, and protein synthesis inhibition. Mol. Pharmacol. 48, 206-211.
21. 2-adrenergic receptor dephosphorylation by vesicular acidification. J. Biol. Chem. 272, 5-8.
22. Lee H. T., Thompson C. I., Hernandez A., Lewy J. L., and Belloni F. L. (1993) Cardiac desensitization to adenosine analogues after prolonged R-PIA infusion in vivo. Am. J. Physiol. 265, H1916-H1927.
23. Leid M., Kastner P., Lyons R., Nakshatri H., Saunders M., Zacharewski T., Chen J.-Y., Staub A., Gamier J.-M., Mader S., and Chambon P. (1992) Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently. Cell 68, 377-395.
24. 1 adenosine receptor-mediated inhibition of cardiac myocyte contractility and adenylate cyclase activity: relation to the regulation of receptor affinity and density. Circ. Res. 67, 406-414.
25. 1 adenosine receptors. FASEB J. 5, 2668-2676.
26. iα are not associated with changes in their mRNAs. Mol. Pharmacol. 36, 681 - 688.
27. Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
28. Novelli A., Reilly J. A., Lysko P. G., and Henneberry R. C. (1988) Glutamate becomes neurotoxic via the N-methyl-D-aspartate when intracellular energy levels are reduced. Brain Res. 451, 205-212.
29. Olah M. E. and Stiles G. L. (1995) Adenosine receptor subtypes: characterization and therapeutic regulation. Annu. Rev. Pharmacol. Toxicol. 35, 581-606.
30. 2A adenosine receptor: delineation of multiple processes. Mnl. Pharmacol. 45, 1082-1094.
31. i. J. Biol. Chem. 262, 841-847.
32. 2-adrenergic receptors permit receptor resensitization. Mol Pharmacol. 47, 666-676.
33. 1 MF-2 cells. Mol. Pharmacol. 40, 639-647.
34. 1 adenosine-receptor phosphorylation by the β-adrenergic receptor kinase. Biochim. Biophys. Acta 1179, 89-97.
35. 2A-adrenergic receptor mRNA in rat astroglial cultures: role of cyclic AMP and protein kinase C. J. Neurochem. 68, 47-57.
36. 2A adenosine receptor mRNA expression by agonists and forskolin in PC12 cells. Neuroreport 5, 1317-1320.
37. Salomon Y. (1979) Adenylate cyclase assay, in Advances in Cyclic Nucleotide Research, Vol. 10 (Brooker G., Greengard P., and Robison G. A., eds), pp. 35-55. Raven Press, New York.
38. 1-adenosine receptors in embryonic chicken heart. Am. J. Physiol. 256, H321-H327.
39. 2-adrenergic receptors between the plasma membrane and endosomes containing transferrin receptors. J. Biol. Chem. 267, 3530-3538.
40. Zhang G., Franklin P. H., and Murray T. F. (1993) Modulation of endogenous adenosine in the rat prepiriform cortex modulates seizure susceptibility. J. Pharmacol. Exp. Ther. 264, 1415-1424.