Biophysical characterization of the stability of the 150-kilodalton botulinum toxin, the nontoxic component, and the 900-kilodalton botulinum toxin complex species

Infection and Immunity

Volumn 66, Issue 6, 1998, Pages 2420-2425

  Chen, Flora ^a   Kuziemko, Geoffrey M ^a,b   Stevens, Raymond C ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOTULINUM TOXIN A; NEUROTOXIN;

ANIMAL CELL; ARTICLE; BIOPHYSICS; CIRCULAR DICHROISM; CLOSTRIDIUM BOTULINUM; HEMAGGLUTINATION; LIGHT SCATTERING; MOLECULAR MODEL; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; RABBIT;

CLOSTRIDIUM BOTULINUM;

EID: 0031866118     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.6.2420-2425.1998     Document Type: Article

References (37)

1. Balding, P., E. R. Gold, D. A. Boroff, and T. A. Roberts. 1973. Observations on receptor specific proteins. II. Haemagglutination and haemagglutination-inhibition reactions of Clostridial botulinum types A, C, D and E haemagglutinins. Immunology 25:773-782.
2. Beck, W. S., K. F. Liem, and A. R. Simpson. 1991. Life, an introduction to biology, 3rd ed., p. 666-676. Harper Collins Publishers, Inc., New York, N.Y.
3. Blasl, J., E. R. Chapman, E. Link, T. Binz, S. Yamasaki, P. De Camill, T. C. Südhof, H. Niemann, and R. Jahn. 1993. Botulinum neurotoxin A selectively cleaves the synaptic protein SNAP-25. Nature 365:160-163.
4. Blaustein, R. O., W. J. Germann, A. Finkelstein, and B. R. DasGupta. 1987. The N-terminal half of the heavy chain of botulinum type A neurotoxin forms channels in planar phospholipid bilayers. FEBS Lett. 226:115-120.
5. Chen, F., G. M. Kuziemko, P. Amersdorfer, C. Wong, J. D. Marks, and R. C. Stevens. 1997. Antibody mapping to domains of botulinum neurotoxin serotype A in the complexed and uncomplexed forms. Infect. Immun. 65: 1626-1630.
6. DasGupta, B. R., and D. A. Boroff. 1968. Separation of toxin and hemagglutinin from crystalline toxin of Clostridium botulinum type A by anion exchange chromatography and determination of their dimensions by gel filtration. J. Biol. Chem. 243:1065-1072.
7. DasGupta, B. R., D. A. Boroff, and E. Rothstein. 1966. Chromatographic fractionation of the crystalline toxin of Clostridium botulinum type A. Biochem. Biophys. Res. Commun. 22:750-756.
8. Datta, A., and B. R. DasGupta. 1988. Circular dichroic and fluorescence spectroscopic study of the conformation of botulinum neurotoxin types A and E. Mol. Cell. Biochem. 79:153-159.
9. Dudeja, P. K., J. M. Harig, K. Ramaswamay, and T. A. Brasitus. 1989. Protein-lipid interaction in human small intestinal brush-border membranes. Am. J. Physiol. 257:G809-G817.
10. Fling, S. P., and D. S. Gregerson. 1988. Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris buffer system without urea. Anal. Biochem. 155:83-88.
11. Fujita, R., Y. Fujinaga, K. Inoue, H. Nakajima, H. Kumon, and K. Oguma. 1995. Molecular characterization of two forms of nontoxic-nonhemagglutinin components of Clostridium botulinum type A progenitor toxins. FEBS Lett. 376:41-44.
12. Hayashi, T., H. McMahon, S. Yamasaki, T. Binz, Y. Hata, T. C. Südhof, and H. Niemann. 1994. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. EMBO J. 2:5051-5061.
13. Heckly, R. J., G. J. Hildebrand, and C. Lamanna. 1960. On the size of the toxic particle passing the intestinal barrier in botulism. J. Exp. Med. 111: 745-759.
14. Inoue, K., Y. Fujinaga, T. Watanabe, T. Ohyama, K. Takeshi, K. Moriishi, H. Nakajima, K. Inoue, and K. Oguma. 1996. Molecular composition of Clostridium botulinum type A progenitor toxins. Infect. Immun. 64:1589-1594.
15. Johnson, L. R. 1981. Physiology of the gastrointestinal tract, p. 625-627. Raven Press Books, Ltd., New York, N.Y.
16. Johnson, L. R. 1981. Physiology of the gastrointestinal tract, p. 784-787. Raven Press Books, Ltd., New York, N.Y.
17. Kamata, Y., Y. Kimura, and S. Kozaki. 1994. Involvement of phospholipids in the intoxication mechanism of botulinum neurotoxin. Biochim. Biophys. Acta 1199:65-68.
18. Kozaki, S., A. Miki, Y. Kamata, T. Nagai, J. Ogasawara, and G. Sakaguchi. 1989. Immunological characterization of the papain-induced fragments of Clostridium botulinum type A neurotoxin and interaction of the fragments with brain synaptosomes. Infect. Immun. 57:2634-2639.
19. Lamanna, C. 1948. Hemagglutination by botulinal toxin. Proc. Soc. Exp. Biol. Med. 69:332-336.
20. Lowenthal, J. P., and C. Lamanna. 1951. Factors affecting the botulinal hemagglutination reaction, and the relationship between hemagglutinating activity and toxicity of toxin preparations. Am. J. Hyg. 54:342-353.
21. Lowenthal, J. P., and C. Lamanna. 1953. Characterization of botulinal hemagglutination. Am. J. Hyg. 57:46-59.
22. May, A. J., and B. C. Whaler. 1958. The absorption of Clostridium botulinum type A toxin from the alimentary canal. Br. J. Exp. Pathol. 39:307-316.
23. Moberg, L. J., and H. Sugiyama. 1978. Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxin complex. Appl. Environ. Microbiol. 35:878-880.
24. Montecucco, C., G. Schiavo, Z. Gao, E. Bauerlein, P. Boquet, and B. R. DasGupta. 1988. Interaction of botulinum and tetanus toxins with the lipid bilayer surface. Biochem. J. 251:379-383.
25. Montecucco, C., G. Schiavo, and B. R. DasGupta. 1989. Effect of pH on the interaction of botulinum neurotoxins A, B and E with liposomes. Biochem. J. 259:47-53.
26. Ohishi, I., S. Sugii, and G. Sakaguchi. 1977. Oral toxicites of Clostridium botulinum in response to molecular size. Infect. Immun. 16:107-109.
27. Schmid, M. F., J. P. Robinson, and B. R. DasGupta. 1993. Direct visualization of botulinum neurotoxin-induced channels in phospholipid vesicles. Nature 364:827-830.
28. Shone, C. C., P. Hambleton, and J. Melling. 1985. Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. Eur. J. Biochem. 151:75-82.
29. Simpson, L. L. 1980. Kinetic studies on the interaction between botulinum toxin type A and the cholinergic neuromuscular junction. J. Pharmacol. Exp. Ther. 212:16-21.
30. Singh, B. R., and B. R. DasGupta. 1989. Structure of heavy and light chain subunits of type A botulinum neurotoxin analyzed by circular dichroism and fluorescence measurements. Mol. Cell. Biochem. 85:67-73.
31. Singh, B. R., B. Li, and D. Read. 1995. Botulinum versus tetanus neurotoxins: why is botulinum neurotoxin but not tetanus neurotoxin a food poison? Toxicon 33:1541-1547.
32. 1, and E produce proteins with or without hemagglutinating activity: do they share common amino acid sequences and genes? J. Protein Chem. 10:415-425.
33. Sugii, S., I. Ohishi, and G. Sakaguchi. 1977. Correlation between oral toxicity and in vitro stability of Clostridium botulinum type A and B toxins of different molecular sizes. Infect. Immun. 16:910-914.
34. Sugii, S., I. Ohishi, and G. Sakaguchi. 1977. Intestinal absorption of botulinum toxins of different molecular sizes in rats. Infect. Immun. 17:491-496.
35. Sugiyama, H. 1980. Clostridium botulinum neurotoxin. Microbiol. Rev. 44: 419-448.
36. Tacket, C. O., and M. A. Rogawski. 1989. Botulism, p. 354-356. In L. L. Simpson (ed.), Botulinum neurotoxin and tetanus toxin. Academic Press, Inc., San Diego, Calif.
37. Wagman, J., and J. B. Bateman. 1953. Botulinum type A toxin: properties of a toxic dissociation product. Arch. Biochem. Biophys. 45:375-383.