Overexpression of mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase does not correct glucose-stimulated insulin secretion from diabetic GK rat pancreatic islets

Diabetologia

Volumn 41, Issue 6, 1998, Pages 649-653

  Ueda, K ^a   Tanizawa, Y ^a   Ishihara, H ^b   Kizuki, N ^a   Ohta, Y ^a   Matsutani, A ^a   Oka, Y ^a  

^a YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Adenovirus; FAD linked glycerol 3 phosphate dehydrogenase; GK rat; Insulin secretion; Non insulin dependent diabetes mellitus

Indexed keywords

GLUCOSE; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; GLYCEROPHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ENZYME ACTIVITY; ENZYME DEFECT; FEMALE; GENETIC DISORDER; GENETIC TRANSDUCTION; GLUCOSE METABOLISM; GLYCOLYSIS; INSULIN RELEASE; MALE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PANCREAS ISLET BETA CELL; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ADENOVIRIDAE; ANIMALS; BLOTTING, WESTERN; DIABETES MELLITUS, EXPERIMENTAL; DIABETES MELLITUS, TYPE 2; DISEASE MODELS, ANIMAL; FEMALE; FLAVIN-ADENINE DINUCLEOTIDE; GENE EXPRESSION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLUCOSE; GLYCEROLPHOSPHATE DEHYDROGENASE; INSULIN; ISLETS OF LANGERHANS; LAC OPERON; MALE; MITOCHONDRIA; RATS; RATS, INBRED STRAINS; RATS, WISTAR; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSFECTION;

EID: 0031865596     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001250050963     Document Type: Article

References (20)

1. Goto Y, Kakizaki M, Masaki N (1975) Spontaneous diabetes produced by selective breeding of normal Wistar rats. Proceedings of the Japan Academy, 51: 80-85
2. Portha B, Serradas P, Bailbe D, Suzuki K, Goto Y, Giroix M-H (1991) Beta cell insensitivity to glucose in the GK rat, a spontaneous nonobese model for type II diabetes. Diabetes 40: 486-491
3. Ward WK, Beard JC, Halter JB, Pfeiffer MA, Porte D (1984) Pathophysiology of insulin secretion in non-insulin dependent diabetes mellitus. Diabetes Care 7: 491-502
4. Östenson C-G, Khan A, Abdel-Halim SM, Guenifi A, Suzuki K, Goto Y, Efendic S (1993) Abnormal insulin secretion and glucose metabolism in pancreatic islets from the spontaneously diabetic GK rat. Diabelologia 36: 3-8
5. Hughes SJ, Suzuki K, Goto Y (1994) The role of islet secretory function in the development of diabetes in the GK Wistar rat. Diabetologia 37: 863-870
6. Ohneda M, Johnson JH, Inman LR et al. (1993) GLUT2 expression and function in beta-cells of GK rats with NIDDM. Diabetes 42: 1065-1072
7. Tsuura Y, Ishida H, Okamoto Y et al. (1993) Glucose sensitivity of ATP-sensitive K channels is impaired in beta-cells of the GK rat. A new genetic model of NIDDM. Diabetes 42: 1446-1453
8. MacDonald MJ (1981) High content of mitochondrial glycerol 3-phosphate dehydrogenase in pancreatic islets and its inhibition by diazoxide. J Biol Chem 256: 8287-8290
9. Meglasson MD, Smith KM, Nelson D, Erecinski M (1989) α-glycerophosphate shuttle in a clonal β-cell line. Am J Physiol 256: E173-E178
10. MacDonald MJ, Warner TF, Pellet JR (1983) Increased mitochondrial glycerol phosphate dehydrogenase activity in insulinomas of two hypoglycemic infants. J Clin Endocrinol Metab 57: 662-664
11. Östenson CG, Abdel-Halim SM, Rasschaert J et al. (1993) Deficient activity of FAD-linked glycerolphosphate dehydrogenase in islets of GK rats. Diabetologia 36: 722-726
12. Giroix M-H, Rasschaert J, Bailbe D, Leclercq-Meyer V, Sener A, Portha B, Malaisse WJ (1991) Impairment of glycerol phosphate shuttle in islets from rats with diabetes induced by neonatal streptozotocin. Diabetes 40: 227-232
13. Sener A, Herberg L, Malaisse WJ (1993) FAD-linked glycerophosphate dehydrogenase deficiency in pancreatic islets of mice with hereditary diabetes. FEBS Lett 316: 224-227
14. Rasschaert J, Malaisse-Lagae F, Sener A, Leclercq-Meyer V, Herberg L, Malaisse WJ (1994) Impaired FAD-glycerophosphate dehydrogenase activity in islet and liver homogenates of fa/fa rats. Mol Cell Biochem 135: 137-141
15. Fernandez-Alvarez J, Conget I, Rasschaert J, Sener A, Gomis R, Malaisse WJ (1994) Enzymatic, metabolic and secretory patterns in human islets of type 2 (non-insulin dependent) diabetic patients. Diabetologia 37: 177-181
16. MacDonald MJ, Efendic S, Östenson C-G (1996) Normalization by insulin treatment of low mitochondrial glycerol phosphate dehydrogenase and pyruvate carboxylase in pancreatic islets of the GK rat. Diabetes 45: 886-890
17. Lacy P, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35-39
18. Ishihara H, Nakazaki M, Kanegae Y et al. (1996) Effect of mitochondrial and/or cytosolic glycerol 3-phosphate dehydrogenase overexpression on glucose-stimulated insulin secretion from MIN6 and HIT cells. Diabetes 45: 1238-1244
19. Oka Y, Asano T, Shibasaki Y, Kasuga M, Kanazawa Y, Takaku F (1988) Studies with antipeptide antibody suggest the presence of at least two types of glucose transporter in rat brain and adipocyte. J Biol Chem 263: 13432-13439
20. Becker TC, Beltrandelrio H, Noel RJ, Johnson JH, Newgard CB (1994) Overexpression of hexokinase I in isolated islets of Langerhans via recombinant adenovirus. J Biol Chem 269: 21234-21238