Improved validated assay for the determination of proguanil and its metabolites in plasma, whole blood, and urine using solid-phase extraction and high-performance liquid chromatography

Therapeutic Drug Monitoring

Volumn 20, Issue 3, 1998, Pages 325-330

  Bergqvist, Yngve ^a,b,c   Funding, Lars ^c   Krysèn, Birgitta ^a   Leek, Tomas ^a   Yvell, Karin ^a  

^a DALARNA UNIVERSITY   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c FALUN HOSPITAL   (Sweden)

Author keywords

Antimalarial drugs; High performance liquid chromatography; Proguanil; Solid phase extraction; Validation

Indexed keywords

1 (3 CHLOROPHENYL)BIGUANIDE; 4 CHLOROPHENYLBIGUANIDE; ACETONITRILE; ANTIMALARIAL AGENT; BUFFER; CHLORCYCLOGUANIL; CYCLOGUANIL; DRUG METABOLITE; METHANOL; PHOSPHATE; PROGUANIL; UNCLASSIFIED DRUG;

ARTICLE; DRUG BLOOD LEVEL; DRUG DETERMINATION; DRUG STABILITY; DRUG URINE LEVEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MALARIA; PRIORITY JOURNAL; SOLID PHASE EXTRACTION;

EID: 0031865399     PISSN: 01634356     EISSN: None     Source Type: Journal    
DOI: 10.1097/00007691-199806000-00014     Document Type: Article

References (18)

1. Helsby NA, Ward SA, Howells RE, Breckenridge AM. In vitro metabolism of the biguanide antimalarials in human liver microsomes: Evidence for a role of the mephenytoin hydroxylase (P450 MP) enzyme. Br J Clin Pharmacol 1990;30:287-91.
2. Ward SA, Helsby NA, Skjelbo E, Brosen K, Gram LF, Breckenridge AM. The activation of the biguanide antimalarial proguanil co-segregates with the mephenytoin oxidation polymorphism: a panel study. Br J Clin Pharmacol 1991;31:689-92.
3. Kelly JA, Fletcher KA. High performance liquid Chromatographic method for the determination of proguanil and cycloguanil in biological fluids. J Chromatogr 1986;381:464-71.
4. Edstein MD. Simultaneous measurement of proguanil and cycloguanil in human plasma by high performance liquid chromatography. J Chromatogr 1986;380:184-9.
5. Wanwimolruk S, Pratt EL. A simple HPLC assay for proguanil and its active metabolite cycloguanil: application to oxidation phenotyping. J Liquid Chromatogr 1995;18:4097-105.
6. Kusaka M, Setiabudy R, Chiba K, Ishizaki T. Simultaneous measurement of proguanil and its metabolites in human plasma and urine by reversed-phase high-performance liquid chromalography, and its preliminary application in relation to genetically determined S-mephenytoin 4'-hydroxylation status. Am J Trop Med Hyg 1996;54(2): 189-96.
7. Musch G, Massart D. Isolation of basic drugs in plasma using solid-phase extraction with a cyanopropyl-bonded phase. J Chromatogr 1988;432:209-22.
8. Chen X, Wijsbeck J, van Veen J, Frauhe JP, De Zeeuw RA. Solid phase extraction for the screening of acidic, neutral and basic drugs in plasma using a single-column procedure. J Chromatogr 1990; 529:161-6.
9. McDowall RD. The universal SPE method. LC-GC-INT 1994;7: 638-40.
10. Taylor RB, Moody RR, Ochekpe NA. Determination of proguanil and its metabolites cycloguanil and 4-chlorophenylbigu-anide in plasma, whole blood and urine by high-performance liquid chromatography. J Chromatogr 1987;416:394-9.
11. Taylor RB, Behrens R, Moody RR, Wangbooskul J. Assay method for the simultaneous determination of proguanil, chloroquine and their main metabolites in biological fluids. J Chromatogr 1990; 527:490-7.
12. Chaulet J-F, Grelaud G, Bellemin-Maigninot P, Mounier C, Brazier J-L. Simultaneous determination of chloroquine. proguanil and their metabolites in human biological fluids by high-performance liquid chromatography. J Pharm Biomed Anal 1994; 12:111-7.
13. Dadgar D, Burnett PE, Choc MG, Gallicano K, Hooper JW. Application issue in bioanalytical method validation, sample analysis and data reporting. J Pharm Biomed Anal 1995;13:89-97.
14. Wieling J, Hendriks G, Tamminga WJ, Hempenius J, Mensink CK, Oosterhuis B, Jonkman JHG. Rational experimental design for bioanalytical methods validation: Illustration using an assay method for total captopril in plasma. J Chromatogr A 1996;730: 381-94.
15. Vessman J. Selectivity or specificity? Validation of analytical methods from the perspective of an analytical chemist in the pharmaceutical industry. J Pharm Biomed Anal 1996;14:867-9.
16. Bygbjerg IC, Flachs H. Effect of oral proguanil on human lymphocyte proliferation. Eur J Clin Pharmacol 1986;30:249-51.
17. Kolawole JA, Mustapha A, Abdul-Aguye I, Ochekpe N. Effect of temperature on chlorproguanil and proguanil hydrochloride solutions: a chemical stability study. J Pharm Biomed Anal 1996;14: 685-93.
18. Taylor RB, Moody RR, Ochekpe NA, Low AS, Harper MIA. A chemical stability study of proguanil hydrochloride. Int J Pharmaceutic 1990;60:185-90.