Application of fluorescence microscopy to measure apoptosis in Jurkat T cells after treatment with a new investigational anticancer agent (N.C.1213)

Cellular and Molecular Neurobiology

Volumn 18, Issue 4, 1998, Pages 437-445

  Vashishtha, S C ^a   Nazarali, A J ^b   Dimmock, J R ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b Laboratory of Molecular Biology

Author keywords

Apoptosis; Fluorescence microscopy; Human Jurkat T cells; Mannich base; Melphalan

Indexed keywords

ANTINEOPLASTIC AGENT; DNA FRAGMENT; MELPHALAN; NC 1213; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL STRUCTURE; CELL SURVIVAL; CHROMATIN CONDENSATION; CYTOTOXICITY; DNA CLEAVAGE; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; NUCLEOSOME; PRIORITY JOURNAL;

EID: 0031863845     PISSN: 02724340     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022505700642     Document Type: Article

References (18)

1. Cohen, G. M., Sun, X.-M., Snowden, R. T., Dinsdale, D., and Skilleter, D. N. (1992). Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem. J. 286:331-334.
2. Collins, R. J., Harmon, B. V., Gobe, G. C., and Kerr, J. F. R. (1992). Internucleosomal DNA cleavage should not be the sole crieterion for identifying apoptosis. Int. J. Radiat Biol. 61:451-453.
3. Cox, G., and Austin, R. C. (1997). Dexamethasone-induced suppression of apoptosis in human neutrophils requires continuous stimulation of new protein synthesis. J. Leukoc. Biol. 61:224-230.
4. Dimmock, J. R., and Kumar, P. (1997). Anticancer and cytotoxic properties of Mannich bases. Curr. Med. Chem. 4:1-22.
5. Dimmock, J. R, Kumar, P., Quail, J. W., Pugazhenthi, U., Yang, J., Chen, M., Reid, R. S., Allen, T. M., Kao, G. Y., Cole, S. P. C., Batist, G., Balzarini, J., and De Clercq, E. (1995). Synthesis and cytotoxic evaluation of some styryl ketones and related compounds. Eur. J. Med. Chem. 30:209-217.
6. Dive, C., and Hickman, J. A. (1991). Drug-target interactions: Only the first step in the commitment to a programmed cell death? Br. J. Cancer 64:192-196.
7. Duke, R. C., and Cohen, J. J. (1992). Morphological and biochemical assays of apoptosis. Curr. Protocols Immunol. Suppl. 3:3.17.1-3.17.16.
8. Dyson, J. E. D., Simmons, D. M., Daniel, J., McLaughlin, J. M., Quirke, P., and Bird, C. C. (1986). Kinetic and physical studies of cell death induced by chemotherapeutic agents or hyperthermia. Cell Tissue Kinet. 19:311-324.
9. Erciyas, E., Erkaleli, H. I., and Cosar, G. (1994). Antimicrobial evaluation of some styryl ketone derivatives and related thiol adducts. J. Pharm. Sci. 83:545-548.
10. Hickman, J. A. (1992). Apoptosis induced by anticancer drugs. Cancer Metastatis. Rev. 11:121-139.
11. Kerr, J. F. R, Winterford, C. M., and Harmon, B. V. (1994). Apoptosis. Its significance in cancer and cancer therapy. Cancer 73:2013-2026.
12. Mutus, B., Wagner, J. D., Talpas, C. J, Dimmock, J. R., Phillips, O. A., and Reid, R. S. (1989). 1-p-Chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one hydrobromide, a sulfhydryl-specific compound which reacts irreversibly with protein thiols but reversibly with small molecular weight thiols. Anal. Biochem. 177:237-243.
13. Nikonova, L. V., Nelipovich, P. A., and Umansky, S. R. (1982). The involvement of nuclear nucleases in rat thymocyte DNA degradation after γ-irradiation. Biochim. Biophys. Acta. 699:281-289.
14. Shimizu, T., Kubota, M., Tanizawa, A., Sano, H., Kasai, Y., Hashimoto, H., Akiyama, Y., and Mikawa, H. (1990). Inhibition of both etoposide-induced DNA fragmentation and activation of poly(ADP-ribose) synthesis by zinc ion. Biochem. Biophys. Res. Commun. 169:1172-1177.
15. Ucker, D. S, Obermiller, P. S., Eckhart, W., Apgar, J. R., Berger, N. A., and Meyers, J. (1992). Genome digestion is a dispensable consequence of physiological cell death mediated by cytotoxic T lymphocytes. Mol. Cell Biol. 12:3060-3069.
16. Vashishtha, S. C. (1996). Mannich Bases and Other Related Compounds Designed Principally as Candidate Cytotoxic and Anticancer Agents, Ph.D., thesis, University of Saskatchewan, Saskatoon.
17. Wyllie, A. H, Kerr, J. F. R., and Currie, A. R. (1980). Cell death: the significance of apoptosis. Int. Rev. Cytol. 68:251-306.
18. Wyllie, A. H., Morris, R. G., Smith, A. L., and Dunlop, D. (1984). Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis. J. Pathol. 142:67-77.