Chemiluminescence detection of nitric oxide production from rat cerebral cortical endothelial cells in culture

Brain Research Protocols

Volumn 2, Issue 3, 1998, Pages 175-182

  Weih, Markus K ^a   Weikert, Steffen ^a   Freyer, Dorette ^a   Dirnagl, Ulrich ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Brain; Constitutive nitric oxide synthase; Nitrite; Ozone

Indexed keywords

BRADYKININ; CALCIUM IONOPHORE; NEUROTRANSMITTER; NITRIC OXIDE;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; BLOOD VESSEL TONE; BRAIN CORTEX; CELL CULTURE; CHEMICAL ANALYSIS; CHEMOLUMINESCENCE; ENDOTHELIUM CELL; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CATTLE; CELLS, CULTURED; CEREBRAL CORTEX; CHEMILUMINESCENT MEASUREMENTS; ENDOTHELIUM, VASCULAR; MICROCIRCULATION; NITRIC OXIDE; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS;

EID: 0031862914     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00037-8     Document Type: Article

References (20)

1. Abbott N.J., Hughes C.C., Revest P.A., Greenwood J. Development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. J. Cell Sci. 103:1992;23-37.
2. Archer S. Measurement of nitric oxide in biological models. FASEB J. 7:1993;349-360.
3. Bowman P.D., Ennis S.R., Rarey K.E., Betz D.L., Goldstein G.W. Brain microvessel endothelial cells in tissue culture: a model for studying blood-brain barrier permeability. Ann. Neurol. 14:1983;396-402.
4. Freyer D., Weih M., Weber J.R., Burger W., Scholz P., Manz R., Ziegenhorn A., Angestwurm K., Dirnagl U. Pneumococcal cell wall components induce nitric oxide synthase and TNF-alpha in astroglial-enriched cultures. Glia. 16:1996;1-6.
5. Gobbel G.T., Chan T.Y., Gregory G.A., Chan P.H. Response of cerebral endothelial cells to hypoxia: modification by fructose-1,6-bisphosphate but not glutamate receptor antagonists. Brain Res. 653:1994;23-30.
6. Griess Dtsch. Chem. Ges. 12:1879;426.
7. Hiraishi H., Terano A., Razandi M., Pedram A., Sugimoto T., Harada T., Ivey K. Reactive oxygen metabolite-induced toxicity to cultured bovine endothelial cells: status of cellular iron in mediating injury. J. Cell Physiol. 160:1994;132-140.
8. Huang Z., Huang P.L., Panahian N., Dalkara T., Fishman M.C., Moskowitz M.A. Effects of cerebral ischemia in mice deficient in neuronal nitric oxide synthase. Science. 265:1994;1883-1885.
9. Koshland D.E. Molecule of the year. Science. 262:1993;1953.
10. Kunz D., Walker G., Eberhardt W., Pfeilschifter J. Molecular mechanisms of dexamethasone inhibition of nitric oxide synthase expression in interleukin 1 beta-stimulated mesangial cells: evidence for the involvement of transcriptional and posttranscriptional regulation. Proc. Natl. Acad. Sci. U.S.A. 93:1996;255-259.
11. Menon N.K., Patricza J., Binder T., Bing R.J. Reduction of biological effluents in purge and trap micro reaction vessels and detection of endothelium-derived nitric oxide by chemiluminescence. J. Mol. Cell. Cardiol. 23:1991;389-393.
12. Menon N.K., Wolf A., Zehetgruber M., Bing R.J., Archer S. An improved chemiluminescence assay suggests non nitric oxide-mediated action of lysophosphatidylcholine and acetylcholine measurement of nitric oxide in biological models. Proc. Soc. Exp. Biol. Med. 7:1993;349-360.
13. Moncada S., Palmer R.M., Higgs E.A. Biosynthesis of nitric oxide from L-arginine. A pathway for the regulation of cell function and communication. Biochem. Pharmacol. 38:1989;1709-1715.
14. Morin A.M., Stanboli A. Nitric oxide synthase localization in cerebro-vascular endothelium during mitosis. Exp. Cell Res. 211:1994;183-188.
15. O'Neill S.K., Dutta S., Triggle C.R. Computerized data acquisition and analysis applied to chemiluminescence detection of nitric oxide in headspace gas. J. Pharmacol. Toxicol. Meth. 29:1993;217-221.
16. Palmer R.M., Ferrige A.G., Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 327:1987;524-526.
17. 2+-independent nitric oxide synthases. FEBS Lett. 291:1991;145-149.
18. S.E. Schwartz, W.H. White (Eds.), Trace Atmospheric Constituents: Properties, Transformation and Fates, Wiley, New York, 1983, pp. 1-21.
19. Termin A., Hoffmann M., Bing R.J. A simplified method for the determination of nitric oxide in biological solutions. Life Sci. 51:1992;1621-1629.
20. 2+-dependent NO production from CNS cells in culture measured by NO-nitrite/ozone chemoluminescence. Brain Res. 748:1997;1-11.