A new test-combination: DAS-ELISA with subsequent amplified ELISA on the same microtitre plate for detection of potato virus X in potato tubers

Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz

Volumn 105, Issue 2, 1998, Pages 105-113

  Darda, G ^a,b  

^a Eidgenossische Forsch Anst F O   (Switzerland)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Amplification; Certification programmes; ELISA; Potato viruses; Routine virus diagnosis; Screening; Seed potato production; Virus indexing

Indexed keywords

ASSAY; BUFFER; DISEASE DETECTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACT; PLANT VIRUS; POTATO; SENSITIVITY; VIRUS INFECTION;

EID: 0031860397     PISSN: 03408159     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. ALRIC, M., D. CHEYVIALLE, M. RENAUD: Cross-Blot and Cross-Dot System: a high-performance system for the detection of antigen-antibody complexes on nitrocellulose. - Analyt. Biochem. 155, 328-334, 1986.
2. BRANDT, S., G. HIMMLER: Detection of nepoviruses in ligneous grapevine material by using IC/PCR. - Vitis 34, 127-128, 1995.
3. CASPER, R., S. MEYER: Die Anwendung des ELISA-Verfahrens zum Nachweis pflanzenpathogener Viren. - NachrBl. dt. PflSchutzdienst Braunschweig 33, 49-54, 1981.
4. CLARK, M. F., A. N. ADAMS: Characteristics of the microplate method of Enzyme-Linked Immunosorbent Assay for the detection of plant viruses. - J. gen. Virol. 34, 475-483, 1977.
5. COMMISSION DIRECTIVE 92/34/EEC: Directive on the marketing of fruit plant propagating material and fruit plants intended for fruit production. - Official Gazette of the European Communities No. L 157, 10-18, 1992.
6. DARDA, G., T. HASLER: Virusnachweis mittels ELISA. - Tätigkeitsbericht 1994/95 der Eidg. Forschungsanstalt für Obst-, Wein- und Gartenbau Wädenswil, Switzerland, 1996a.
7. DARDA, G., T. HASLER: Prüfung einer Kombination aus Standard- und amplifiziertem ELISA auf Eignung für den schnellen Nachweis von Viren in Obstgehölzen bei Reihenuntersuchungen. -Phytomedizin 26, 55-56, 1996b.
8. DARDA, G., E. SANDER: Comparison of detection limit of PVX (potato virus X) in a new combination: DAS-ELISA and subsequent "amplified ELISA" in the same well, with ELISA variants, Cross-Dot and PVX-RNA hybridization. - Abstr. 6th Intern. Congr. of Plant Path., Montreal, Canada, 45, July 28-Aug. 6, 1993.
9. DE BOER, S. H., S. A. SLACK, G. W. VAN DEN BOVENKAMP, I. MASTENBROEK: A role for pathogen indexing procedures in potato certification. - In: Callow, J. A. (ed.): Adv. bot. Res. 23, Academic Press London, U. K., 1996.
10. EUROP. AND MEDIT. PLANT PROT. ORG. (EPPO): Certification scheme: virus-free or virus-tested fruit trees and rootstocks, part. I. - EPPO Bull. 21, 267-277, 1991.
11. EUROP. AND MEDIT. PLANT PROT. ORG. (EPPO): Certification scheme: virus-free or virus-tested fruit trees and rootstocks, part II, III and IV. - EPPO Bull. 22, 255-283, 1992.
12. EWEIDA, M., H. Xu, R. P. SINGH, M. G. ABOUHAIDAR: Comparison between ELISA and biotin-labelled probes from cloned cDNA of potato virus X for the detection of virus in crude tuber extracts. - Pl. Path. 39, 623-628, 1990.
13. HENSON, J. M., R. FRENCH: The polymerase chain reaction and plant disease diagnosis. - Ann. Rev. Phytopath. 31, 81-109, 1993.
14. HUTTINGA, H.: Sensitivity of indexing procedures for viruses and viroids. - In: CALLOW, J. A. (ed.): Adv. bot. Res. 23, Academic Press London, U.K., 1996.
15. JOHANNSSON, A.: Enzyme amplification of immunoassays: increased speed and sensitivity. - Focus 10, 49-53, 1988.
16. KÖHM, B.: Rapid and simple detection of plant viruses by reverse passive haemagglutination; II. Detection of four potato viruses (PVM, PVS, PVX and PVY) in potato tubers and foliage by RPH (reverse passive haemagglutination) and a comparison with ELISA (Enzyme-Linked Immunosorbent Assay). - Z. PflKrankh. PflSchutz 100, 243-249, 1993.
17. KOENIG, R., D. E. LESEMANN: Potato virus X. - In: MURANT, A. F., B. D. HARRISON (eds.): CMI/AAB Descriptions of plant viruses 354, Association of Applied Biologists, Wellesbourne Warwick, U.K., 1989.
18. KOENIG, R., J. H. TREMAINE, J. F. SHEPARD: In situ degradation of the protein chain of potato virus X at the N- and C-termini. - J. gen. Virol. 38, 329-337, 1978.
19. LAMPRECHT, S., D. KADEN-KREUZIGER, W. JELKMANN: Immunocapture polymerase chain reaction (IC-PCR) und ELISA-Nachweis des strawberry mild yellow edge associated potexvirus (SMYEAV). - Mitt. Biol. Bundesanst. Land-Forstwirtsch. (Berlin-Dahlem), H. 301, 169, 1994.
20. LISTER, R. M.: Apple chlorotic leafspot virus. - In: MURANT, A. F., B. D. HARRISON (eds.): CMI/AAB Descriptions of plant viruses 30, Association of Applied Biologists, Wellesbourne Warwick, U. K., 1970a.
21. LISTER, R. M.: Apple stem grooving virus. - In: MURANT, A. F., B. D. HARRISON (eds.): CMI/AAB Descriptions of plant viruses 31, Association of Applied Biologists, Wellesbourne Warwick, U. K., 1970b.
22. LOEBENSTEIN, G., F. AKAD, V. FILATOV, G. SADVAKASOVA, A. MANADILOVA, H. BAKELMAN, E. TEVEROVSKY, O. LACHMANN, A. DAVID: Improved detection of potato leafroll luteovirus in leaves and tubers with a digoxigenin-labeled cRNA probe. - Pl. Dis. 81, 489-491, 1997.
23. MATTHEWS, R. E. F.: Plant virology. - Academic Press London, U. K., third ed., 1991.
24. MEYER-KASHNITZ, S.: Angewandte Pflanzenvirologie. - Bernhard Thalacker Verlag Braunschweig, Germany, 1993.
25. NOLASCO, G., C. DE BLAS, V. TORRES, F. PONZ: A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. - J. Virol. Methods 45, 201-218, 1993.
26. SINGH, R. P., T. H. SOMERVILLE: Evaluation of the enzyme-amplified ELISA for the detection of potato viruses A, M, S, X, Y, and leafroll. - Am. Potato J. 69, 21-30, 1992.
27. STANLEY, C. J., A. JOHANNSSON, C. H. SELF: Enzyme amplification can enhance both the speed and sensitivity of immunoassays. - J. Immunol. Methods 83, 89-95, 1985.
28. STEAD, D. E., D. L. EBBELS, A. W. PEMBERTON: The value of indexing for disease control strategies. -In: CALLOW, J. A. (ed.): Adv. bot. Res. 23, Academic Press London, U. K., 1996.
29. TORRANCE, L.: Use of enzyme amplification in an ELISA to increase sensitivity of barley yellow dwarf virus in oats and in individual vector aphids. - J. Virol. Methods 15, 131-138, 1987.
30. VAN DEN HEUVEL, J. F. J. M., D. PETERS: Improved detection of potato leafroll virus in plant material and in aphids. - Phytopathology 79, 963-967, 1989.
31. VETTEN, H. J., U. EHLERS, H. L. PAUL: Detection of potato virus Y and A in tubers by Enzyme-Linked Immunosorbent Assay after natural and artificial break of dormancy. - Phytopath. Z. 108, 41-53, 1983.
32. VOLLER, A., A. BARTLETT, D. E. BIDWELL, M. F. CLARK, A. N. ADAMS: The detection of viruses by Enzyme-Linked Immunosorbent Assay (ELISA). - J. gen. Virol. 33, 165-167, 1976.
33. WEIDEMANN, H.-L.: Nachweis von Kartoffelvirus Y und Kartoffelvirus in Kartoffelproben mit zwei ELISA-Varianten. - NachrBl. dt. PflSchutzdienst Braunschweig 46, 263-266, 1994.
34. WETZEL, T., T. CANDRESSE, G. MACQUAIRE, M. RAVELONANDRO, J. DUNEZ: A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. - J. Virol. Methods 39, 27-37, 1992.