Use of rotofor preparative isoelectrofocusing cell in protein purification procedure

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 69, Issue 1, 1998, Pages 11-16

  Ayala, Antonio ^a   Parrado, Juan ^a   Machado, Alberto ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

6 phosphogluconate dehydrogenase; Rotofor; Superoxide dismutase

Indexed keywords

PHOSPHOGLUCONATE DEHYDROGENASE; SUPEROXIDE DISMUTASE;

ARTICLE; BRAIN HOMOGENATE; COMPLEX FORMATION; CONTAMINATION; ENZYME PURIFICATION; ISOELECTRIC FOCUSING; PROTEIN DENATURATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SUPERNATANT;

EID: 0031855512     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02786017     Document Type: Article

References (11)

1. Ayala, A., F-Lobato, M., and Machado, A. (1986), FEBS Lett. 202, 102-106.
2. Ayala, A., Fabregat, I., and Machado, A. (1990), Mol. Cell. Biochem. 95, 107-115.
3. Ayala, A., Gordillo, E., Castaño, A., Lobato, M.-F., and Machado, A. (1991), Biochim. Biophys. Acta 1084, 48-52.
4. Gordillo, E., Ayala, A., F-Lobato, M., Bautista, J., and Machado, A. (1988), J. Biol. Chem. 263, 8053-8057.
5. Gordillo, E., Ayala, A., Bautista, J., and Machado, A. (1989), J. Biol. Chem. 264, 17,014-17,019.
6. Egen, N. B., Thormann, W., Twitty, G. E., and Bier, M. (1984), in Electrophoresis 83, Hirai, H., ed., De Gruyter, Berlin, pp. 547-550.
7. Egen, N. B., Bliss, M., Mayersohn, M., Owens, S. M., Arnold, L., and Bier, M. (1988), Anal. Biochem. 172, 488-494.
8. Hochstrasser, A. C., James, R. W., Pometta, D., and Hochstrasser, D. (1990), Appl. Theoret. Electrophoresis 1, 333-337.
9. Laemmli, U. K. (1970), Nature 270, 680-685.
10. McCord, J. M. and Fridovich, I. (1969), J. Biol. Chem. 244, 6049-6055.
11. Sapag-Hagar, M., Lagunas, R., and Sols, A. (1973), Biochem. Biophys. Res. Commun. 50, 179-185.