Disruption of the actin cytoskeleton of mammalian cells by the capping complex actin-fragmin is inhibited by actin phosphorylation and regulated by Ca2+ ions

Journal of Cell Science

Volumn 111, Issue 12, 1998, Pages 1695-1706

  Constantin, Bruno ^a,b   Meerschaert, Kris ^a   Vandekerckhove, Joël ^a   Gettemans, Jan ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b UNIVERSITÉ DE POITIERS   (France)

Author keywords

Actin cytoskeleton; Actin phosphorylation; Actin binding protein; Microinjection; Transfection

Indexed keywords

ACTIN; CALCIUM ION; DALTEPARIN; LYSOPHOSPHATIDIC ACID; PROTEIN SUBUNIT;

ACTIN POLYMERIZATION; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL STRUCTURE; CYTOSKELETON; FLUORESCENCE MICROSCOPY; MICROINJECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

3T3 CELLS; ACTINS; ANIMALS; BUFFERS; CALCIUM; CARRIER PROTEINS; CATIONS, DIVALENT; CELL LINE; CYTOSKELETON; EPITHELIAL CELLS; FIBROBLASTS; INTERPHASE; MICE; MICROFILAMENT PROTEINS; MICROINJECTIONS; PHOSPHORYLATION; RECOMBINANT PROTEINS; TRANSFECTION;

ANIMALIA; CHONDROMYCES; MAMMALIA; PHYSARUM; PHYSARUM POLYCEPHALUM;

EID: 0031852863     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Brix, K. and Stockem, W. (1989). Functional analysis of actin fibrils in Physarum polycephalum. A morphological and quantitative study. Cell Tissue Res. 257, 115-122.
2. Brock, A. M. and Pardee, J. D. (1988). Cytoimmunofluorescent localization of severin in Dictyostelium amoebae. Dev. Biol. 128, 30-39.
3. Cooper, J. A. (1987). Effects of cytochalasin and phalloidin on actin. J. Cell Biol. 105, 1473-1478.
4. Cooper, J. A., Bryan, J., Schwab, III, B., Frieden, C., Loftus, D. J. and Elson, E. L. (1987). Microinjection of gelsolin into living cells. J. Cell Biol. 104, 491-501.
5. Croop, J. and Holtzer, H. (1975). Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. J. Cell Biol. 65, 271-285.
6. Cunningham, C. C., Stossel, T. P. and Kwiatkowski, D. J. (1991). Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science 251, 1233-1236.
7. De Corte V., Gettemans, J., Waelkens, E. and Vandekerckhove, J. (1996). In vivo phosphorylation of actin in Physarum polycephalum. Study of the substrate specificity, of the actin-fragmin kinase. Eur. J. Biochem. 241, 901-908.
8. Eichinger, L., Bomblies, L., Vandekerckhove, J., Schleicher, M. and Gettemans, J. (1996). A novel type of protein kinase phosphorylates actin in the actin-fragmin complex. EMBO J. 15, 5547-5556.
9. Finidori, J., Friederich, E., Kwiatkowski, D. J. and Louvard, D. (1992). In vivo analysis of functional domains from villin and gelsolin. J. Cell Biol. 116, 1145-1155.
10. Füchtbauer, A., Jockush, B. M., Maruta, H., Kilimann, M. W. and Isenberg, G. (1983). Disruption of microfilament organization after injection of F-actin capping proteins into living tissue culture cells. Nature 304, 361-364.
11. Gettemans, J., De Ville, Y., Vandekerckhove, J. and Waelkens, E. (1992). Physarum actin is phosphorylated as the actin-fragmin complex at residues Thr203 and Thr202 by a specific 80 kDa kinase. EMBO J. 11, 3185-3191.
12. Gettemans, J., De Ville, Y., Vandekerckhove, J. and Waelkens, E. (1993). Purification and partial amino acid sequence of the actin-fragmin kinase from Physarum polycephalum. Eur. J. Biochem. 214, 111-119.
13. Gettemans, J., De Ville, Y., Vandekerckhove, J. and Waelkens, E. (1995). The actin-binding properties of the actin-fragmin complex. Regulation by calcium, phospholipids and phosphorylation. J. Biol. Chem. 270, 2644-2651.
14. Hasegawa, T., Takahashi, S., Hayashi, H. and Hatano, S. (1980). Fragmin: A calcium ion sensitive regulatory factor on the formation of actin filaments. Biochemistry 19, 2677-2683.
15. 2+-signaling in dendrites of pyramidal neurons. Biophys. J. 70, 1069-1081.
16. Hinssen, H. (1981a). An actin modulating protein from Physarum polycephalum. I. Isolation and purification Eur. J. Cell. Biol. 23, 225-233.
17. 2+-dependence and other properties. Eur. J. Cell Biol. 23, 234-240.
18. Huckriede, A., Hinssen, H., Füchtbauer, A., Chaponnier, C. and Weeds, A. (1990). Differential effects of gelsolins on tissue culture cells. Cell Motil. Cytoskel. 16, 229-238.
19. 2+mobilizing stimulus for fibroblasts. Evidence for an extracellular site of action. J. Biol. Chem. 265, 12232-12239.
20. Janmey, P. (1994). Phosphomositides and calcium as regulators of cellular actin assembly and disassembly. Annu. Rev. Physiol. 56, 169-191.
21. 2+ transients induced in Paramecium cells by a polyamine secretagogue. J. Cell Sci. 110, 975-983.
22. Kukulies, J., Brix, K. and Stockem, W. (1985). Fluorescent analog cytochemistry of the actin system and cell surface morphology in Physarum microplasmodia. Eur. J. Cell Biol. 39, 62-69.
23. Kukulies, J., Brix, K. and Stockem, W. (1987). Studies on microplasmodia of Physarum polycephalum. VI. Functional analysis of a cortical and fibrillar actin system by use of fluorescent-analog cytochemistry. Cell Tissue Res. 250, 125-134.
24. Mertens, N., Remaut, E. and Fiers, W. (1995). Versatile, multi-featured plasmids for high-level expression of heterologous genes in Escherichia coli: overproduction of human and murine cytokines. Gene 164, 9-15.
25. Mitchison, T. J. and Cramer, L. P. (1996). Actin-bascd cell motility and cell locomotion. Cell 84, 371-379.
26. Neher, E. (1995). The use of Fura-2 for estimating Ca buffer and Ca fluxes. Neuropharmacology 34, 1423-1442.
27. Ridley, A. and Hall, A. (1992). The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389-399.
28. Sampath, P. and Pollard, T. D. (1991). Effects of cytochalasin, phalloidin and pH on the elongation of actin filaments. Biochemistry 30, 1973-1980.
29. Schafer, D. A. and Cooper, J. A. (1995). Control of actin assembly at filament ends. Annu. Rev. Cell. Dev. Biol. 11, 497-518.
30. Schliwa, M. (1982). Action of cytochalasin D on cytoskeletal networks. J. Cell Biol. 92, 79-91.
31. Stockem, W. and Brix, K. (1994). Analysis of microfilament organization and contractile activities in Physarum. Int. Rev. Cytol. 149, 145-215.
32. Stossel, T. P. (1989). From signal to pseudopod. How cells control cytoplasmic actin assembly. J. Biol. Chem. 264, 18261-18264.
33. Stossel, T. P. (1993). On the crawling of animal cells. Science 260, 1086-1094.
34. T'Jampens, D., Meerschaert, K., Constantin, B., Bailey, J., Cook, L. J., De Corte, V., Gevaert, K., De Mol, H., Goethals, M., Van Damme, J., Vandekerckhove, J. and Gettemans, J. (1997). Molecular cloning, over-expression, development regulation and immunolocalization of fragmin. a gelsolin-related actin-binding protein from Physarum polycephalum plasmodia. J. Cell Sci. 110, 1215-1226.
35. Waelkens, E., Gettemans, J., De Corte, V., De Ville, Y., Goris, J., Vandekerckhove, J. and Merlevede, W. (1995). Microfilament dynamics Regulation of actin polymerization by actin-fragmin kinase and phosphatases. Advan. Enzyme Regul. 35, 199-227.
36. Wodnicka, M., Pierzchalska, M., Bereiter-Hahn, J. and Kajstura, J. (1992). Comparative study on effects of cytochalasins B and D on F-actin content in different cell lines and different culture conditions. Folia Histochem. Cytobiol. 30, 107-112.