Use of fluorochrome-tagged taxol® to produce fluorescent microtubules in solution

BioTechniques

Volumn 25, Issue 2, 1998, Pages 188-192

  Melan, Melissa A ^a  

^a DUQUESNE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUOROCHROME; PACLITAXEL; TUBULIN;

ARTICLE; BINDING SITE; FLUORESCENCE MICROSCOPY; MICROTUBULE; NONHUMAN;

EID: 0031851580     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98252bm02     Document Type: Article

References (7)

1. Howard, J. and A.A. Hyman. 1993. Preparation of marked microtubules for the assay of polarity of microtubule-based motors by fluorescence microscopy, p. 105-113. In J.M. Scholey (Ed.), Methods in Cell Biology, Vol. 39. Academic Press, New York.
2. Hyman, A., D. Dreschel, D. Kellogg, S. Salser, K. Swain, P. Steffen, L. Wordeman and T. Mitchison. 1991. Preparation of modi- fied tubulins, p. 478-485. In R.B. Vallee (Ed.), Methods in Enzymology, Vol. 196. Academic Press, New York.
3. Nogales, E., S.G. Wolf and K.H. Downing. 1998. Structure of the αβ tubulin dimer by electron crystallography. Nature 391:199-203.
4. Schiff, P.B., J. Fant and S.B. Horwitz. 1979. Promotion of microtubule assembly in vitro by taxol. Nature 277:665-667.
5. Schiff, P.B. and S.B. Horwitz. 1981. Taxol assembles tubulin in the absence of guanosine 5′-triphosphate or microtubule-associated pro- teins. Biochemistry 20:3247-3252.
6. Wang, Y.L. 1989. Fluorescent analog cytochemistry: tracing functional protein components in living cells, p. 1-12. In Y.L. Wang and D.L. Taylor (Eds.), Methods in Cell Biology, Vol. 29. Academic Press, New York.
7. Wani, M.C., H.L. Taylor, M.E. Wall, P. Coggon and A.T. McPhail. 1971. Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J. Am. Chem. Soc. 93:2325-2327.