Cdc2-independent induction of premature mitosis by okadaic acid in HeLa cells

Experimental Cell Research

Volumn 242, Issue 1, 1998, Pages 1-9

  Ghosh, S ^b   Paweletz, N ^a   Schroeter, D ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY OF CALCUTTA   (India)

Author keywords

Cyclins; HeLa cells; Nek2; Okadaic acid; Premature mitesis

Indexed keywords

CYCLIN B; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE 1; PROTEIN KINASE;

ARTICLE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELL NUCLEUS MEMBRANE; CYTOSKELETON; ENZYME ACTIVATION; ENZYME INHIBITION; HELA CELL; MITOSIS; PREMATURE CHROMOSOME CONDENSATION; PRIORITY JOURNAL;

EID: 0031848567     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1998.4115     Document Type: Article

References (42)

1. Dunphy, W. G., Brizuela, L., Beach, D., and Newport, W. (1988). The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis. Cell 54, 423-431.
2. Gautier, J., Norbury, C., Lokha, M., Nurse, P., and Maller, J. (1988). Purified maturation promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2. Cell 54, 433-439.
3. Draetta, G., Luca, F., Westendorf, J., Brizuela, L., Ruderman, J., and Beach, D. (1989). Cdc2 protein kinase is complexed with both cyclin A and B, evidence for proteolytic inactivation of MPF. Cell 56, 829-838.
4. Labbé, J. C., Capony, J. P., Daniel, C., Cavadore, J. C., Davencourt, J., Kaghad, M., Lelias, J. M., Picard, A., and Doreé, M. (1989). MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B. EMBO J. 8, 3053-3058.
5. Hartwell, L. H., and Weinert, T. A. (1989). Checkpoint: Controls that ensure the order of cell cycle events. Science 246, 629-634.
6. Schlegel, R., and Pardee, A. (1986). Caffeine induced uncoupling of mitosis from the completion of DNA replication in mammalian cells. Science 232, 1264-1266.
7. Steinmann, K. E., Belinsky, G. S., Lee, D., and Schlegel, R. (1991). Chemically induced premature mitosis: Differential response in rodent and human cells and the relationship to cyclin B synthesis and p34cdc2/cyclin B complex formation. Proc. Natl. Acad. Sci. USA 88, 6843-6847.
8. Cohen, P., and Cohen, P. T. W. (1989). Protein phosphatases come of age. J. Biol. Chem. 21, 435-438.
9. Yamashita, K., Yasuda, H., Pines, J., Yasumoto, K., Nishitani, H., Ohtsubo, M., Hunter, T., Sugimura, T., and Nishimoto, T. (1990). Okadaic acid, a potent inhibitor of type 1 and type 2A protein phosphatases, activates cdc/H1 kinase and transiently induces a premature mitosis like state in BHK21 cells. EMBO J. 9, 4331-4338.
10. Guo, X. G., Th'ng, J. P. H., Swank, R. A., Anderson, H. J., Tudan, C., Bradbury, E. M., and Roberge, M. (1995). Chromosome condensation induced by fostriecin does not require p34cdc2 kinase activity and histone H1 phosphorylation but is associated with enhanced histone H2A and H3 phosphorylation. EMBO J. 14, 976-978.
11. 2-M phase transition in pachytene spermatocytes includes events unique to meiosis. Dev. Biol. 169, 557-567.
12. Ghosh, S., Paweletz, N., and Schroeter, D. (1992). Failure of kinetochore development and spindle formation in okadaic acid induced premature mitosis in HeLa cells. Exp. Cell Res. 201, 535-540.
13. 2-phase to induce premature mitosis in HeLa cells. Exp. Cell Res. 227, 165-169.
14. Gotoh, E., and Asakawa, Y. (1996). Detection and evaluation of chromosomal aberrations induced by high doses of gamma-irradiation, using immunogold silver painting of prematurely condensed chromosomes. Int. J. Radiat. Biol. 70, 517-520.
15. Smythe, C., and Newport, J. W. (1992). Coupling of mitosis to the completion of S-phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2. Cell 68, 787-797.
16. Norbury, C., and Nurse, P. (1992). Animal cell cycles and their control. Annu. Rev. Biochem. 61, 441-470.
17. Li, Y. M., and Casida, J. E. (1992). Cantharidic acid binding protein: Identification as protein phosphatase 2A. Proc. Natl. Acad. Sci. USA 89, 11867-11870.
18. Knehr, M., Poppe, M., Enulescu, M., Eickelbaum, W., Stöhr, M., Schroeter, D., and Paweletz N. (1995). A critical appraisal of synchronisation methods applied to achieve maximal enrichment of HeLa cells in specific cell cycle phases. Exp. Cell Res. 217, 546-553.
19. Kumagai, A., and Dunphy, W. G. (1992). Regulation of the cdc25 protein during the cell cycle in Xenopus extracts. Cell 70, 139-151.
20. Kumagai, A., and Dunphy, W. G. (1991). The cdc25 protein controls tyrosine dephosphorylation of the cdc25 protein in a cell free system. Cell 64, 903-914.
21. Guo, X. G., Th'ng, J. P. H., Swank, R. A., and Bradbury, E. M. (1994). Effect of phosphatase inhibitors on mammalian p34cdc2 kinase activities. In "The Cell Cycle Regulators, Targets and Clinical Applications" (V. W. Hu, Ed.), pp. 41-49, Plenum, New York.
22. Rhee, K., and Woglemuth, D. J. (1997). The NIMA related kinase Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. Development 124, 2167-2177.
23. Fry, A. M., Schultz, S. J., Bartek, J., and Nigg, E. A. (1995). Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans. J. Biol. Chem. 270, 12899-12905.
24. Gadbois, D. M., Hamaguchi, J. R., Swank, R. A., and Bradbury, E. M. (1992). Staurosporine is a potent inhibitor of p34cdc2 and p34cdc-like kinases. Biochem. Biophys. Res. Commun. 184, 80-85.
25. Glotzer, M., Murray, A. W., and Kirschner, M. W. (1991). Cyclin is degraded by the ubiquitin pathway. Nature 349, 132-138.
26. Mueller, R. D., Yasuda, H., Hatch, C. L., Bonner, W. M., and Bradbury, E. M. (1985). Identification of ubiquitinated histones 2A and 2B in Physarum polycephalum. Disappearance of these proteins at metaphase and reappearance at anaphase. J. Biol. Chem. 260, 5147-5153.
27. Ho, D. T., and Roberge, M. (1996). The anti tumour drug fostriecin induces vimentin hyperphosphorylation and intermediate filament reorganisation. Carcinogenesis 17, 967-972.
28. + in fission yeast mitotic control. Cell 57, 1009-1016.
29. Ajiro, K., Yoda, K., Utsumi, K., and Nishikawa, Y. (1996). Alteration of cell cycle dependent histone phosphorylations by okadaic acid. J. Biol. Chem. 271, 13197-13201.
30. Ghosh, S., Paweletz, N., and Schroeter, D. (1993). Failure of centromere separation leads to formation of diplochromosomes in next mitosis in okadaic acid treated HeLa cells. Cell Biol. Int. 17, 949-952.
31. Brinkley, B. R., Zinkowski, R. P., Mollon, W. L., Davis, F. M., Pisegna, M. A., Pershouse, M., and Rao, P. N. (1988). Movement and segregation of kinetochores experimentally detached from mammalian chromosomes. Nature 336, 251-254.
32. Paweletz, N., Finze, E. M., Schroeter, D., Enulescu, M., and Knehr, M. (1995). Immunoelectron microscopic studies on centromere-kinetochore complexes detached from chromosomes. Chromosome Res. 3, 235-238.
33. Chou, Y. H., Bischoff, J. R., Beach, D., and Goldman, R. D. (1990). Intermediate filament reorganisation during mitosis is mediated by the p34cdc2 phosphorylation of vimentin. Cell 62, 1093-1071.
34. Yamashiro, S., Yamakita, Y., Hosoya, H., and Matsumara, F. (1991). Phosphorylation of nonmuscle caldesmon by p34cdc2 kinase during mitosis. Nature 349, 251-253.
35. Gotoh, Y., Nishida, E., Matsuda, S., Shiina, N., Kosako, H., Shiokawa, K., Akiyama, T., Ohta, K., and Sakai, H. (1991). In vitro effects of microtubule dynamics of purified Xenopus M-phase activated MAP-kinase. Nature 349, 251-253.
36. 2 specific gene that encodes a potential protein kinase. Cell 53, 237-243.
37. O'Connell, M. J., Norbury, C., and Nurse, P. (1994). Premature chromatin condensation upon accumulation of NIMA. EMBO J. 13, 4926-4937.
38. Lu, K. P., and Hunter, T. (1995). Evidence for a NIMA-like mitotic pathway in vertebrate cells. Cell 81, 413-424.
39. Schultz, S. J., Fry, A. M., Sütterlin, C., Ried, T., and Nigg, E. A. (1994). Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. Cell Growth Differ. 5, 625-635.
40. Tanaka, K., Parvinen, M., and Nigg, E. A. (1997). The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis. Exp. Cell Res. 237, 264-274.
41. 34cdc2/cyclin B: Coordination with two mitosis promoting kinases. EMBO J. 14, 986-994.
42. 34cdc2 cell cycle regulated protein is required to initiate mitosis in Aspergillus nidulans. Cell 67, 283-291.