Direct use of synthetic peptides for antiserum production

BioTechniques

Volumn 25, Issue 1, 1998, Pages 28-32

  Sheibani, Nader ^a   Frazier, William A ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; ANTISERUM; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; SYNTHETIC PEPTIDE;

ANTIBODY PRODUCTION; ARTICLE; GENE EXPRESSION SYSTEM; HYBRIDIZATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

EID: 0031847638     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98251bm05     Document Type: Article

References (7)

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2. Johnson, K.S., G.B. Harrison, M.W. Lightowlers, K.L. O'Hoy, W.G. Cougle, R.P. Dempster, S.B. Lawrence, J.G. Vinton, D.D. Heath and M.D. Rickard. 1989. Vaccination against ovine cysticercosis using a defined recombinant antigen. Nature 338:585-587.
3. Oettinger, H.F., R. Pasqualini and M. Bernfield. 1992. Recombinant peptides as immunogens: a comparison of protocols for antisera production using the pGEX system. BioTechniques 12:544-549.
4. Qiagen. 1997. The QIAexpressionist, 3rd ed., p. 11. Chatsworth, CA.
5. Sheibani, N. and B.L. Allen-Hoffmann. 1994. Production of recombinant human involucrin using the glutathione-transferase fusion system, p. 167-174. In I.M. Leigh and F. M. Watt (Eds.), Keratinocyte Methods. Cambridge University Press, Cambridge, UK.
6. Smith, D.B. and K.S. Johnson. 1988. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase. Gene 67:31-40.
7. Toye, B., G. Zhong, R. Peeling and R.C. Brunham. 1992. Immunologic characterization of a cloned fragment containing the species-specific epitope from the major outer membrane protein of Chlamydia trachomatis. Infection Immunol. 58:3909-3913.