Modification of the MTT method for the study of bilirubin cytotoxicity

Pediatrics International

Volumn 40, Issue 4, 1998, Pages 313-317

  Ngai, Kin Chun ^a   Yeung, Chap Yung ^a   Karlberg, Johan ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Bilirubin; Cell viability assay; Cytotoxicity; Mitochondrial function assay; Modified MTT method

Indexed keywords

2 PROPANOL; 3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BILIRUBIN; FORMAZAN; HYDROCHLORIC ACID;

ARTICLE; CELL VIABILITY; COLORIMETRY; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; LIVER CELL; MITOCHONDRION; OPTICAL DENSITY; PRIORITY JOURNAL;

2-PROPANOL; BILIRUBIN; CELLS, CULTURED; COLORING AGENTS; FORMAZANS; HUMANS; HYDROCHLORIC ACID; LIVER; METHODS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 0031846693     PISSN: 13288067     EISSN: 1442200X     Source Type: Journal    
DOI: 10.1111/j.1442-200X.1998.tb01938.x     Document Type: Article

References (14)

1. Garnter Lm, Catz Cs, Yaffe Sj. Neonatal bilirubin workshop. Pediatrics 1994; 94: 537–40.
2. Brodersen R. Bilirubin: Solubility and interaction with albumin and phospholipid. J. Biol. Chem. 1979; 254: 2364–9.
3. Brodersen R. Bilirubin transport in the newborn infant, reviewed with relation to kernicterus. J. Pediatr. 1980; 94: 349–56.
4. Duerst Re, Frantz Cn. A sensitive assay of cytotoxicity applicable to mixed cell populations. J. Immunol. Methods 1985; 82: 39–46.
5. Lichtenstein A., Kahle J., Bonavida B. Use of a self-generating Percoll gradient and single cell cytotoxicity assay to identify tumorlytic properties of inflammatory neutrophils. J. Immunol. Method. 1985; 81: 95–106.
6. Filman Dj, Brawn Rj, Dandliker Wb. Intracellular supravital stain derealization as an assay for antibody-dependent complement-mediated cell damage. J. Immunol. Method. 1975; 6: 189–207.
7. 51Crlabelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology 1968; 14: 181–96.
8. Shirhatti V., Krishna G. A simple and sensitive method for monitoring drug-induced cell injury in culture cells. Anal. Biochem. 1985; 147: 410–18.
9. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983; 65: 55–63.
10. Schiff D., Chan G., Poznansky Mj. Bilirubin toxicity in neural cell lines N115 and NBR 10A. Pediatr. Res. 1985; 19: 908–11.
11. 35S]-methionine incorporation and mitochondrial function. Pediatr. Res. 1989; 25: 364–8.
12. Amit Y., Poznansky Mj, Schiff D. Bilirubin toxicity in a neuroblastoma cell line N-115: II. Delayed effects and recovery. Pediatr Res. 1989; 25: 369–72.
13. Denizot F., Lang R. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Method. 1986;89: 271–7.
14. Brodersen R., Theilgaard J. Bilirubin colloid formation in neutral aqueous solution. Scand. J. Clin. Lab. Invest. 1969; 24: 395–8.