A modification for the improved analysis of differential protein accumulation by two-dimensional polyacrylamide gel electrophoresis

Phytochemical Analysis

Volumn 9, Issue 4, 1998, Pages 159-161

  Ekramoddoullah, Abul K M ^a   Tan, Yingchun ^a  

^a PACIFIC FORESTRY CENTRE   (Canada)

Author keywords

Conifer proteins; Two dimensional gel electrophoresis; White pine blister rust

Indexed keywords

COMPUTER AIDED ANALYSIS; CROSSLINKING; ELECTROPHORESIS; SODIUM COMPOUNDS;

CONIFER PROTEIN; GEL ELECTROPHORESIS; PHENOLIC COMPOUNDS; PROTEIN ACCUMULATION; PROTEIN SAMPLES; TWO DIMENSIONAL (2 D); TWO-DIMENSIONAL GEL ELECTROPHORESIS; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; WHITE PINE; WHITE PINE BLISTER RUST;

PROTEINS;

DODECYL SULFATE SODIUM; PHENOL DERIVATIVE; PIPERAZINE DERIVATIVE; VEGETABLE PROTEIN;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; CROSS LINKING; ISOELECTRIC FOCUSING; NONHUMAN; PINE; PROTEIN ANALYSIS; TREE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CONIFEROPHYTA; CRONARTIUM FLACCIDUM; CRONARTIUM RIBICOLA;

EID: 0031844046     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1565(199807/08)9:4<159::AID-PCA399>3.0.CO;2-F     Document Type: Article

References (6)

1. Ekramoddoullah, A. K. M. (1991). Analysis of proteins of western white pine (Pinus monticola Dougl.) needles. In Rusts of Pine. Proc. 3rd IUFRO Rusts of Pine Working Party Conference, 1989, Banff, Alberta. (Hiratsuka, Y., Samoil, J. K., Blenis, P. V., Crane, P. E. and Laishley, B. L., eds), pp. 102-108. Canadian Forest Service, Edmonton.
2. Ekramoddoullah, A. K. M. and Hunt, R. S. (1993). Changes in protein profile of susceptible and resistant sugar pine foliage infected with white pine blister rust fungus, Cronartium ribicola. Can. J. Plant Pathol. 15, 259-264.
3. Ekramoddoullah, A. K. M. and Davidson, J. J. (1995). A method for the determination of conifer foliage protein extracted using sodium dodecyl sulphate and mercaptoethanol. Phytochem, Anal. 6, 20-24.
4. Hochstrasser, D. F., Harrington, M. G., Hochstrasser, A. C., Miller, M. J. and Merril, C. R. (1988). Method for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424-435.
5. Millipore Corporation (1993). Investigator 2D Electrophoresis System, Operating and Maintenance Manual. Millipore Corporation, Bedford, MA, USA.
6. Rabilloud, T. (1992). A comparison between low background silver diamine and silver nitrate protein stains. Electrophoresis 13, 429-439.