Rapid fluorescence-based measurement of neutrophil migration in vitro

Journal of Immunological Methods

Volumn 213, Issue 1, 1998, Pages 41-52

  Frevert, C W ^a   Wong, V A ^a   Goodman, R B ^a   Goodwin, R ^b   Martin, T R ^a  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b Neuro Probe   (United States)

Author keywords

C5a; Calcein AM; Chemotaxis; Fluorescence; Interleukin 8; Leukocyte; Migration; Neutrophil

Indexed keywords

CALCEIN; COMPLEMENT COMPONENT C5A; INTERLEUKIN 8;

ARTICLE; BIOASSAY; CHEMOTAXIS; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; LEUKOCYTE MIGRATION; NEUTROPHIL; NORMAL HUMAN; PRIORITY JOURNAL;

CELL COUNT; CHEMOTAXIS, LEUKOCYTE; EVALUATION STUDIES; FLUORESCEINS; FLUORESCENCE; FLUORESCENT DYES; HUMANS; MICROPORE FILTERS; NEUTROPHILS; POLYCARBOXYLATE CEMENT; POLYESTERS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

EID: 0031843837     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00016-7     Document Type: Article

References (22)

1. DeForge L.E., Kenney J.S., Jones M.L., Warren J.S., Remick D.G. Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. Separation of LPS- and cytokine-stimulated components using anti-tumor necrosis factor and anti-IL-1 antibodies. J. Immunol. 148:1992;2133.
2. Frevert C.W., Warner A.E., Kobzik L. Defective pulmonary recruitment of neutrophils in a rat model of endotoxemia. Am. J. Respir. Cell. Mol. Biol. 11:1994;716.
3. Frevert C.W., Huang S., Danaee H., Paulauskis J.D., Kobzik L. Functional characterization of the rat chemokine KC and its importance in neutrophil recruitment in a rat model of pulmonary inflammation. J. Immunol. 154:1995;335.
4. Goodman R.B., Forstrom J.W., Osborn S.G., Chi E.Y., Martin T.R. Identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages. J. Biol. Chem. 266:1991;8455.
5. Haddox J.L., Knowles I.W., Sommers C.I., Pfister R.R. Characterization of chemical gradients in the collagen gel-visual chemotactic assay. J. Immunol. Meth. 171:1994;1.
6. Harvath L., Falk W., Leonard E.J. Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multi-well assembly. J. Immunol. Meth. 37:1980;39.
7. Harvath L., Brownsom N.E., Fields G.B., Skubitz A.P.N. Laminin peptides stimulate human neutrophil motility. J. Immunol. 152:1994;5447.
8. Haugland, R., 1996. pH indicators. In Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes, p. 551.
9. Junger W.G., Cardoza T.A., Liu F.C., Hoyt D.B., Goodwin R. Improved rapid photometric assay for quantitative measurement of PMN migration. J. Immunol. Meth. 160:1993;73.
10. Krauss A.H., Nieves A.L., Spada C.S., Woodward D.F. Determination of leukotriene effects on human neutrophil chemotaxis in vitro by differential assessment of cell motility and polarity. J. Leukocyte Biol. 55:1994;201.
11. Leonard E.J., Yoshimura T., Rot A., Noer K., Walz A., Baggiolini M., Walz-D A., Goetzl E.J., Castor C.W. Chemotactic activity and receptor binding of neutrophil attractant/activation protein-1 (NAP-1) and structurally related host defense cytokines: interaction of NAP-2 with the NAP-1 receptor. J. Leukocyte Biol. 49:1991;258.
12. Milton, J.S., Tsokos, J.O., 1983. Statistical Methods in the Biological and Health Sciences. McGraw-Hill, New York, NY.
13. Milton et al., 1983.
14. Pillemer L., Blum L., Lepow I., Wurz L., Todd E.W. Properdin system and immunity: zymosan assay of properdin. J. Exp. Med. 103:1956;1.
15. Psychoyos S., Uziel-Fusi S., Morrissey M., Ganu V., Smith C. Thick filter neutrophil chemotaxis performed in the absence of albumin. J. Immunol. Meth. 137:1991;37.
16. Quan J.M., Martin T.R., Rosenberg G.B., Foster D.C., Whitmore T., Goodman R.B. Antibodies against the N-terminus of IL-8 receptor A inhibit neutrophil chemotaxis. Biochem. Biophys. Res. Commun. 219:1996;405.
17. Rot A. Chemotactic potency of recombinant human neutrophil attractant/activation protein-1 (interleukin-8) for polymorphonuclear leukocytes of different species. Cytokine. 3:1991;21.
18. Shi Y., Kornovski B.S., Savani R., Turley E.A. A rapid, multi-well colorimetric assay for chemotaxis. J. Immunol. Meth. 164:1993;149.
19. Smart S.J., Casale T.B. Interleukin-8-induced transcellular neutrophil migration is facilitated by endothelial and pulmonary epithelial cells. Am. J. Respir. Cell. Mol. Biol. 9:1993;489.
20. Vaporciyan A.A., Jones M.L., Ward P.A. Rapid analysis of leukocyte-endothelial adhesion. J. Immunol. Meth. 159:1993;93.
21. Zicha D., Dunn G.A., Brown A.F. A new direct-viewing chemotaxis chamber. J. Cell Sci. 99:1991;769.
22. Zigmond S.H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J. Cell Biol. 75:1977;606.