Tightly controlled two-stage expression vectors employing the FLP/FRT- medicated inversion of cloned genes

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 9, Issue 1, 1998, Pages 17-24

  Sektas, Marian ^a   Szybalski, Waclaw ^b  

^a UNIVERSITY OF GDA SK   (Poland)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Escherichia coli; Inversion cassette; tet promoter; Two stage control

Indexed keywords

PLASMID VECTOR;

ARTICLE; CHROMOSOME INVERSION; ESCHERICHIA COLI; GENE EXPRESSION; GENOME; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; REPORTER GENE;

EID: 0031841246     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02752694     Document Type: Article

References (30)

1. Makrides, S. C. (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60, 512-538.
2. Hillen, W. and Berens, C. (1994) Mechanisms underlying expression of Tn10-encoded tetracycline resistance. Annu. Rev. Microbiol. 48, 345-369.
3. Szybalski, W. (1993) From the double-helix to novel approaches to the sequencing of large genomes. Gene 135, 279-290.
4. Pósfai, G., Koob, M., Hradečná, Z., Hasan, N., Filutowicz, M., and Szybalski, W. (1994) In vivo excision and amplification of large segments of the Escherichia coli genome. Nucleic Acids Res. 22, 2392-2398.
5. Wild, J., Hradecná, Z., Póstai, G., and Szybalski, W. (1996) A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50-to 100-kb genomic fragments for direct DNA sequencing. Gene 179, 181-188.
6. Podhajska, A. J., Hasan, N., and Szybalski, W. (1985) Control of cloned gene expression by promoter inversion in vivo: construction of the heat-pulse-activated att-nutL-p-att-N module. Gene 40, 163-168.
7. tac promoter. Gene 56, 145-151.
8. Cox, M. M. (1988) FLP site-specific recombination system of Saccharomyces cerevisae, in Genetic Recombination, vol. 1 (Kucherlapati, R. and Smith, G. R., eds.), American Society for Microbiology, Washington, DC, pp. 429-443.
9. Futcher, A. B. (1988) The 2 μm circle plasmid of Saccharomyces cerevisiae. Yeast 4, 27-40.
10. Kilby, N. J., Snaith, M. R., and Murray, J. A. H. (1993) Site-specific recombinase: tools for genome engineering. Trends Genet. 9, 413-421.
11. Plasterk, R. H. A., Ilmer, T. A. M., and Van De Putte, P. (1983) Site-specific recombination by Gin of bacteriophage Mu: inversions and deletions. Virology 127, 24-36.
12. Tijhaar, E. J., Zheng-Xin, Y., Karlas, J. A., Meyer, T. F., Stukart, M. J., Osterhaus, A. D. M. E., and Mooi, F. R. (1994) Construction and evaluation of an expression vector allowing the stable expression of foreign antigens in a Salmonella typhimurium vaccine strain. Vaccine 12, 1004-1011.
13. d'Oliveira, C., Tijhaar, E. J., Shiels, B. R., Van der Weide, M., and Jongejan, F. (1996) Expression of genes encoding two major Theileria annulata merozoite surface antigens in Escherichia coli and a Salmonella typhimurium aroA vaccine strain. Gene 172, 33-39.
14. Huber, H. E., Iida, S., and Bickle, T. A. (1985) Expression of the bacteriophage P1 cin recombinase gene from its own and heterologous promoters. Gene 34, 63-72.
15. Dale, E. C. and Ow, D. W. (1990) Intra-and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91, 79-85.
16. Casabadan, M. J. (1980) Analysis of gene control signals by DNA fusion in Escherichia coli. J. Mol. Biol. 138, 179-207.
17. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Pribnow, D. (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc. Natl. Acad. Sci. USA 72, 784-788.
19. Lisser, S. and Margalit, H. (1993) Compilation of E. coli mRNA promoter sequences. Nucleic Acids Res. 21, 1507-1516.
20. Simons, R. W., Houman, F., and Kleckner, N. (1987) Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53, 85-96.
21. Greener, A., Filutowicz, M. S., McEachern, M. J., and Helinski, D. R. (1990) N-terminal truncated forms of the bifuncional π initiation protein express negative activity on plasmid R6K replication. Mol. Gen. Genet. 224, 24-32.
22. Durland, R. H., Toukdarian, A., Fang, F., and Helinski, D. R. (1990) Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers. J. Bacteriol. 172, 3859-3867.
23. Andrews, B. J., Proteau, G. U., Beatty, L. G., and Sadowski, P. D. (1985) The FLP recombinase of the 2μ circle DNA of yeast: interaction with its target sequences. Cell 40, 795-803.
24. Miller, J. H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 352-355.
25. Wu, F., Goldberg, I., and Filutowicz, M. (1992) Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K. Nucleic Acids Res. 20, 811-817.
26. Chang, A. C. Y. and Cohen, S. N. (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from P15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156.
27. Kim, J., Zwieb, C., Wu, C., and Adhya, S. (1989) Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector. Gene 85, 15-23.
28. Senecoff, J. F., Bruckner, R. C., and Cox, M. M. (1985) The FLP recombinase of the yeast 2-μm plasmid: characterization of its recombination site. Proc. Natl. Acad. Sci. USA 82, 7270-7274.
29. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., and Falkow, S. (1977) Construction and characterization of new cloning vehicles, II: a multipurpose cloning system. Gene 2, 95-113.
30. Sutcliffe, J. F. (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp. Quant. Biol. 43, 77-90.