High-level expression of the endo-β-N-acetylglucosaminidase F2 gene in E. coli: One step purification to homogeneity

Glycobiology

Volumn 8, Issue 6, 1998, Pages 633-636

  Reddy, Anthony ^a   Grimwood, Brian G ^a   Plummer, T H ^a   Tarentino, Anthony L ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Endo F2; F.meningosepticum; Inclusion bodies; MBP fusion protein

Indexed keywords

HYBRID PROTEIN; MALTOSE BINDING PROTEIN; N ACETYL BETA GLUCOSAMINIDASE; N ACETYL BETA GLUCOSAMINIDASE F2; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL INCLUSION; CHRYSEOBACTERIUM MENINGOSEPTICUM; DIALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; DNA, RECOMBINANT; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GENETIC VECTORS; MANNOSYL-GLYCOPROTEIN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY, AMINO ACID; SUBCELLULAR FRACTIONS; TRANSFECTION;

CHRYSEOBACTERIUM MENINGOSEPTICUM; ESCHERICHIA COLI; FLAVOBACTERIUM; PROKARYOTA;

EID: 0031839784     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/8.6.633     Document Type: Article

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