The role of osteoblast density and endogenous interleukin-6 production in osteoclast formation from the hemopoietic stem cell line FDCP-mix C2GM in coculture with primary osteoblasts

Calcified Tissue International

Volumn 63, Issue 1, 1998, Pages 57-62

  De Grooth, R ^a   Kawilarang de Haas, E W M ^a   Van De Sande Rijkers, C M T ^a   Nijweide, P J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cell density; Interleukin 6; Macrophage; Osteoblast; Osteoclast

Indexed keywords

ALKALINE PHOSPHATASE; BETA 2 MICROGLOBULIN; CALCITRIOL; DNA; INTERLEUKIN 6; INTERLEUKIN 6 RECEPTOR; MESSENGER RNA; RECOMBINANT INTERLEUKIN 6;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL DIFFERENTIATION; CELL LINE; COCULTURE; CONTROLLED STUDY; ENZYME ACTIVITY; FETUS; HEMATOPOIETIC STEM CELL; MOUSE; NONHUMAN; OSTEOBLAST; OSTEOCLAST; PRIORITY JOURNAL;

ANIMALS; CELL COUNT; CELL DIFFERENTIATION; CELL LINE; CELLS, CULTURED; COCULTURE TECHNIQUES; HEMATOPOIETIC STEM CELLS; INTERLEUKIN-6; MACROPHAGES; MICE; OSTEOBLASTS; OSTEOCLASTS; SKULL;

EID: 0031839757     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002239900489     Document Type: Article

References (18)

1. Stein GS, Lian JB, Stein JL, van Wijnen AJ, Frenkel B, Montecino M (1996) Mechanisms regulating osteoblast proliferation and differentiation. In: Bilezikian JP, Raisz LG, Rodan GA (eds) Principles of bone biology. Academic Press, San Diego, California, pp 69-86
2. Suda T, Udagawa N, Takahashi N (1996) Cells of bone: osteoclast generation. In: Bilezikian JP, Raisz LG, Rodan GA (eds). Principles of bone biology. Academic Press, San Diego, California, pp 87-102
3. Spooncer E, Heyworth CM, Dunn A, Dexter TM (1986) Self-renewal and differentiation of interleukin-3-dependent multipotent stem cells are modulated by stromal cells and serum factors. Differentiation 31:111-118
4. Hagenaars CE, Kawilarang-de Haas EWM, van de Kraan AAM, Spooncer E, Dexter TM, Nijweide PJ (1991) Interleukin-3-dependent haemopoietic stem cell lines capable of osteoclast formation in vitro. J Bone Miner Res 6:947-954
5. De Grooth R, Mieremet RHP, Kawilarang-de Haas EWM, Nijweide PJ (1994) Murine macrophage precursor cell lines are unable to differentiate into osteoclasts: a possible implication for the osteoclast ontogeny. Int J Exp Pathol 75:265-275
6. Bazill GW, Haynes M, Garland J, Dexter TM (1983) Characterization and partial purification of a haemopoietic cell growth factor in WEHI-3 cell conditioned medium. Biochem J 210:747-759
7. Modderman WE, Vrijheid-Lammers T, Löwik CWGM, Nijweide PJ (1994) Removal of hemopoietic cells and macrophases from mouse bone marrow cultures: isolation of fibroblast-like stromal cells. Exp Hematol 22:194-201
8. Austyn JM, Gordon S (1981) F4/80, a monoclonal antibody directed against mouse macrophage. Eur J Immunol 11:805-815
9. Lowry OH, Roberts NR, Wu X, Hixon WS, Crawford EJ (1954) The quantitative histochemistry of the brain. II. Enzyme measurements. J Biol Chem 207:19-37
10. Karstens K, Wollenberger A (1977) Improvements in the ethidium bromide method for the direct fluorimetric estimation of DNA and RNA in cell and tissue homogenates. Anal Biochem 77:464-470
11. Aarden L, Lansdorp P, De Groot P (1985) A growth factor for β-cell hybridomas produced by human monocytes. Lymphokines 10:175-185
12. Chomzcynski P, Sacchi N (1987) Single-step method of RNA isolation by acid-guanidiumthiocyanate-phenol-chloroform extraction. Anal Biochem 62:156-159
13. Bellows CG, Aubin JE, Heersche JNM, Antosz ME (1986) Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int 38: 143-154
14. Tamura T, Udagawa N, Takahashi N, Miyaura C, Tanaka S, Yamada Y, Koishihara Y, Ohsugi Y, Kumaki K, Taga T, Kishimoto T, Suda T (1993) Soluble interleukin-6 receptor triggers osteoclast formation by interleukin-6. Proc Natl Acad Sci USA 90:11924-11928
15. Roodman GD (1992) Interleukin-6: an osteotropic factor? J Bone Miner Res 7:475-478
16. Udagawa N, Takahashi N, Katagari T, Tamura T, Wada S, Findlay DM, Martin TJ, Hirota H, Taga T, Kishimoto T, Suda T (1995) Interleukin (IL)-6 induction of osteoclast differentiation depends on IL-6 receptors expressed on osteoblastic cells but not on osteoclast progenitors. J Exp Med 182:1461-1468
17. Udagawa N, Takahashi N, Akatsu T, Sasaki T, Yamaguchi A, Kodama H, Martin TJ, Suda T (1989) The bone marrow-derived stromal cell lines MC3T3-G2/PA6 and ST2 support osteoclast-like cell differentiation in coculture with mouse spleen cells. Endocrinology 125:1805-1813
18. Yamashita T, Ishii H, Shimoda K, Sampath TK, Katagiri T, Wada M, Osawa T, Suda T (1996) Subcloning of three osteoblastic cell lines with distinct differentiation phenotypes from the mouse osteoblastic cell line KS-4. Bone 19:429-436