Perifusion analysis of insulin secretion from specific pathogen-free large-white pig islets shows satisfactory functional characteristics for xenografts in humans

Diabetes and Metabolism

Volumn 24, Issue 3, 1998, Pages 208-214

  Gouin, E ^a,b   Rivereau, A S ^a   Duvivier, V ^c   Darquy, S ^c   Larher, E ^a   You, S ^a   Jestin, A ^d   Reach, G ^c   Sai P ^a  

^a UNIVERSITÉ DE NANTES   (France)

^b Centre Technique des Productions Animales et Agro Alimentaires CTPA   (France)

^c INSERM   (France)

^d Healthy Pigs Production and Experimentation Section   (France)

Author keywords

Insulin secretion; Islets; Pig; Xenograft

Indexed keywords

3 HYDROXYBUTYRIC ACID; ADRENALIN; ARGININE; CARBACHOL; CLONIDINE; GASTRIC INHIBITORY POLYPEPTIDE; GLUCOSE; INSULIN; POTASSIUM; THEOPHYLLINE; TOLBUTAMIDE;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CONTROLLED STUDY; DIABETES MELLITUS; INSULIN RELEASE; NONHUMAN; PANCREAS ISLET BETA CELL; PANCREAS ISLET TRANSPLANTATION; SWINE; SYMPATHETIC TONE; XENOGRAFT;

ANIMALS; GERM-FREE LIFE; HUMANS; INSULIN; PERFUSION; PROGNOSIS; RISK FACTORS; SECRETORY RATE; SWINE; TRANSPLANTATION, HETEROLOGOUS;

EID: 0031839744     PISSN: 03381684     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Groth CG, Korsgren O, Tibell A, Tollemar J, Moller E, Bolinder J, Ostman J, Reinholt FP, Hellerstrom C, Andersson A. Transplantation of porcine fetal pancreas to diabetic patients. Lancet, 1994, 344, 1402-1404.
2. Michaels MG, Simmons RL. Xenotransplant-associated zoonoses. Transplantation, 1994, 57, 1-7.
3. Morris PJ. Pig transplants postponed. Br Med J, 1997, 314, 242.
4. Gouin E, Rivereau AS, Darquy S, Cariolet R, Jestin A, Reach G, Saï P. Minimisation of microbial contamination for potential islet xenografts using specific pathogen-free pigs and a protected environment during tissue preparation. Diabetes Metab, 1997, 23, 537-540.
5. Crowther NJ, Gotfredsen CF, Moody AJ, Green IC. Immunological and insulin secretory studies on isolated porcine islets of Langerhans. J Endocrinol, 1990, 126, 43-49.
6. Marchetti P, Giannarelli R, Di Carlo A, Zappella A, Masoni A, Masiello P, Marchetti A, Picaro L, Navalesi R. In vitro function of porcine islets of Langerhans. Diabetes, Nutrition and Metabolism : Clinical and Experimental, 1989, 2, 105-109.
7. Efendic S, Cerasi E, Luft R, Gladnikoff G. Potentiation of glucose-induced insulin release by glucose in the isolated pancreas of fed and fasted rats. Diabetes, 1976, 25, 949-954.
8. Marchetti P, Swanson C, McLear M, Finke E, Gerasimidi-Vazeou A, Scharp DW, Lacy PE. In vitro function and xenotransplantation of long-term (3 weeks) cultured porcine islets of Langerhans. Transplant Proc, 1992, 24, 637.
9. Cariolet R. Bilan de 10 années d'utilisation de pores exempts d'organismes pathogènes spécifiques (E.O.P.S.) à la station de pathologie porcine de Ploufragan. Journées de Recherche porcine en France, 1986, 18, 321-330.
10. Arbet-Engels C, Darquy S, Capron F, Pueyo ME, Dimaria S, Poitout V, Reach G. A one-step, operator-independent method for isolating islets of Langerhans from the porcine pancreas. Artif Organs, 1994, 18, 570-575.
11. Latif ZA, Noel J, Alejandro R. A simple method of staining fresh and cultured islets. Transplantation, 1988, 45, 827-830.
12. Harrison DE, Christie MR, Gray DW. Properties of isolated human islets of Langerhans : insulin secretion, glucose oxidation and protein phosphorylation. Diabelologia, 1985, 28, 99-103.
13. Turk J, Hughes JH, Easom RA, Wolf BA, Scharp DW, Lacy PE, McDaniel ML. Arachidonic acid metabolism and insulin secretion by isolated human pancreatic islets. Diabetes, 1988, 37, 992-996.
14. Davalli AM, Pontiroli AE, Socci C, Bertuzzi F, Fattor B, Braghi S, Di Carlo V, Pozza G. Human islets chronically exposed in vitro to different stimuli become unresponsive to the same stimuli given acutely : Evidence supporting specific desensitization rather than β-cell exhaustion. J din Endocrinol Metab, 1992, 74, 790-794.
15. Takei S, Teruya M, Grunewald A, Garcia R, Chan EK, Charles MA. Isolation and function of human and pig islets. Pancreas, 1994, 9, 150-156.
16. Wise MH, Yates A, Gordon C, Johnson RWG. Subzero preservation of mechanically prepared porcine islets of Langerhans : response to a glucose challenge in vitro. Cryobiology, 1983, 20, 211-218.
17. Ricordi C, Finke EH, Lacy PE. A method for the mass isolation of islets from the adult pig pancreas. Diabetes, 1986, 35, 649-653.
18. Marchetti P, Zappella A, Giannarelli R, Masiello P, Masoni A, Casanovi E, Gattai V, Pozzolini L, Marchetti A, Navalesi R. Isolation of islets of Langerhans from the adult pig pancreas. Transplant Proc, 1988, 20, 707-708.
19. McLear M, Marchetti P, Finke E, Gerasimidi-Vazeou A, Scharp DW, Lacy PE. Porcine islet function : effects of arginine, leucine, and butyrate. Transplant Proc, 1992, 24, 980-982.
20. Strubbe JH, Steffens AB. Neural control of insulin secretion. Horm Metab Res, 1993, 25, 507-512.
21. Brunicardi FC, Shavelle DM, Andersen DK. Neural regulation of the endocrine pancreas. Int J Pancreatol, 1995, 18, 177-195.
22. Mukherjee B, Chatterjee AK, Bhatia GS, Mukherjee SK. Effect of epinephrine and norepinephrine on immuno-reactive insulin secretion from isolated islets of Langerhans. Biochem Pharmacol, 1985, 34, 985-987.
23. Lacey RJ, Cable HC, James RF, London NJ, Scarpello JH, Morgan NG. Concentration-dependent effects of adrenaline on the profile of insulin secretion from isolated human islets of Langerhans. J Endocrinol, 1993, 138, 555-563.
24. Gregersen H, Jensen SL, Ahren B. An alpha 1-adrenoceptor-sensitive mechanism is responsible for the adrenergic inhibition of insulin secretion in the pig pancreas. Eur J Pharmacol, 1991, 200, 365-367.