An alternative for purification of low soluble recombinant hepatitis C virus core protein: Preparative two-dimensional electrophoresis

Electrophoresis

Volumn 19, Issue 8-9, 1998, Pages 1300-1305

  Yvon, Stéphane ^a   Rolland, Dominique ^a   Charrier, Jean Philippe ^a   Jolivet, Michel ^a  

^a BIOMÉRIEUX   (France)

Author keywords

Hepatitis C; Preparative isoelectric focusing; Preparative two dimensional polyacrylamide gel Electrophoresis; Protein solubility; Recombinant core protein

Indexed keywords

ELECTROPHORESIS; ESCHERICHIA COLI; LIQUID CHROMATOGRAPHY; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; VIRUSES;

CORE PROTEINS; HEPATITIS C; HEPATITIS C VIRUS; ISOELECTRIC FOCUSING; PREPARATIVE ISOELECTRIC FOCUSING; PREPARATIVE TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SOLUBILITY; RECOMBINANT CORE PROTEIN; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS;

PURIFICATION;

CORE PROTEIN;

CONFERENCE PAPER; ESCHERICHIA COLI; HEPATITIS C VIRUS; IMMUNOGENICITY; ISOELECTRIC FOCUSING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0031836244     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150190814     Document Type: Conference Paper

References (28)

1. Choo, Q.-L., Kuo, G., Weiner, A. J., Overby, L. R., Bradley, D. W., Houghton, M., Science 1989, 244, 359-362.
2. Aach, R. D., Stevens, C. E., Hollinger, F. B., Mosley, J. W., Peterson, D. A., Taylor, P. E., Johnson, R. G., Barbosa, L. H., Nemo, G. J., N. Engl. J. Med. 1991, 325, 1325-1329.
3. Alter, M. J., Margolis, S. H., Krawczynski, K., Hudson, F. N., Mares, A., Alexander, J., Hu, P.-Y., Miller, J. K., Gerber, M. A., Samplier, R. E., Meeks, E. L., Beach, M. J., N. Engl. J. Med. 1992, 327, 1899-1905.
4. Hijikata, M., Kalo, N., Ootsuyama, Y., Nakagawa, M., Shimotohno, K., Proc. Natl. Acad. Sci. USA 1991, 88, 5547-5551.
5. Matsumoto, M., Hsieh, T.-Y., Zhu, N., VanArsdale, T., Hwang, S. B., Jeng, K.-S., Gorbalenya, A. E., Lo, S.-Y., Ou, J.-H., Ware, C. F., Lai, M. M., J. Virol. 1997, 71, 1301-1309.
6. Nasoff, M. S., Zebedee, S. L., Inchauspé, G., Prince, A. M., Proc. Natl. Acad. Sci. USA 1991, 88, 5462-5466.
7. Goeser, T., Müller, H. M., Ye, J., Pfaff, E., Theilmann, L., Virology 1994, 205, 462-469.
8. Hitomi, Y., McDonnel, W. M., Baker, J. R., Askari, F. K., Viral Immunol. 1995, 8, 109-119.
9. Matsumoto, M., Hwang, S. B., Jeng, K.-S., Zhu, N., Lai, M. M. C., Virology 1996, 218, 43-51.
10. Khanna, A., Ray, R., Gene 1995, 153, 185-189.
11. Nakagari, I., Ichihara, K., J. Virol. Methods 1995, 52, 195-207.
12. Handschuh, G., Caselmann, W. H., J. Hepatol. 1995, 22, 143-150.
13. Oswald, T., Hornbostel, G., Rinas, U., Anspach, F. B., Biotechnol. Appl. Biochem. 1997, 25, 109-115.
14. Jacobs, E., Clad, A., Anal. Biochem. 1986, 154, 583-589.
15. Laemmli, U. K., Nature 1970, 227, 680-685.
16. Kyte, J., Doolittle, R. F., J. Mol. Biol. 1982, 157, 105-132.
17. Jameson, B. A., Wolf, H., Comput. Appl. Biosci. 1988, 4, 181-186.
18. Das, A., Methods. Enzymol. 1990, 182, 93-113.
19. Marston, F. A. O., Biochem. J. 1986, 240, 1-12.
20. Buchner, J., Pastan, I., Brinkmann, U., Anal. Biochem. 1992, 205, 263-270.
21. Rabilloud, T., Electrophoresis 1996, 17, 813-829.
22. Righetti, P. G., Wenisch, E., Faupel, M., J. Chromatogr. 1989, 475, 293-310.
23. Righetti, P. G., Wenisch, E., Jungbauer, A., Katinger, H., Faupel, M., J. Chromatogr. 1990, 500, 681-696.
24. Ettori, C., Righetti, P. G., Chiesa, C., Frigerio, F., Galli, G., Grandi, G., J. Biotechnol. 1992, 25, 307-318.
25. Esteve-Romero, J. S., Bossi, A., Righetti, P. G., Electrophoresis 1996, 17, 1242-1247.
26. Kraus, C., Klein, E., Ballhausen. W. G., Appl. Theoret. Electrophor. 1993, 3, 271-275.
27. Sandri, M., Rizzi, C., Catani, C., Carraro, U., Anal. Biochem. 1993, 213, 34-39.
28. Dong, M., Baggetto, L. G., Falson, P., Le Maire, M., Penin, F., Anal. Biochem. 1997, 247, 333-341.