A thioredoxin fusion protein of VanH, a D-lactate dehydrogenase from Enterococcus faecium: Cloning, expression, purification, kinetic analysis, and crystallization

Protein Science

Volumn 7, Issue 5, 1998, Pages 1147-1155

  Stoll, Vincent S ^a,c,d   Manohar, A Vaito ^a   Gillon, Wanda ^a,c   Macfarlane, Emma L A ^a,e   Hynes, Rosemary C ^a   Pai, Emil F ^a,b,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c PRINCESS MARGARET HOSPITAL   (Canada)

^d ABBOTT LABORATORIES   (United States)

^e UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Antibiotic resistance; D lactate dehydrogenase; Enterococcus faecium; Enzyme kinetics; Protein crystallization; Vancomycin

Indexed keywords

ADENOSINE DIPHOSPHATE RIBOSE; AMMONIUM SULFATE; ANION; ENTEROPEPTIDASE; HYBRID PROTEIN; LACTATE DEHYDROGENASE; OXAMIC ACID; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; THIOREDOXIN; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; COMPETITIVE INHIBITION; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIFFRACTION; ENTEROCOCCUS FAECALIS; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME PURIFICATION; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SYNCHROTRON;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; STREPTOCOCCUS;

EID: 0031836054     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070508     Document Type: Article

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