Overexpression of high-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1 in recombinant Rhodococcus cells

Applied Microbiology and Biotechnology

Volumn 49, Issue 5, 1998, Pages 568-572

  Mizunashi, W ^a,b   Nishiyama, M ^a   Horinouchi, S ^a   Beppu, T ^a,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b Central Research Laboratory   (Japan)

^c NIHON UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CROTONIC ACID DERIVATIVE; NITRILE HYDRATASE;

ARTICLE; DNA FLANKING REGION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; GEL FILTRATION CHROMATOGRAPHY; GENE CLUSTER; GENE OVEREXPRESSION; MOLECULAR WEIGHT; NONHUMAN; PLASMID; PROMOTER REGION; RHODOCOCCUS; RHODOCOCCUS RHODOCHROUS;

DOSE-RESPONSE RELATIONSHIP, DRUG; HYDRO-LYASES; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; RHODOCOCCUS;

EID: 0031834299     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051214     Document Type: Article

References (15)

1. Asano V, Tani Y, Yamada H (1980) A new enzyme, nitrile hydratase, which degrades acetonitrile in combination with amidase. Agric Biol Chem 44: 2251-2252
2. Burnett WN (1981) 'Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein. Anal Biochem 112: 195-203
3. Duran R, Nishiyama M, Horinouchi S, Beppu T (1993) Characterization of nitrile hydratase genes cloned by DNA screening from Rhodococcus erythropolis. Biosci Biotechnol Biochem 57: 1323-1328
4. Hashimoto Y, Nishiyama M, Ikehata O, Horinouchi S, Beppu T (1991) Cloning and characterization of an amidase gene from Rhodococcus species N-774 and its expression in Escherichia coli. Biochim Biophys Acta 1088: 225-233
5. Hashimoto Y, Nishiyama M, Yu F, Watanabe I, Horinouchi S, Beppu T (1992) Development of a host-vector system in a Rhodococcus strain and its use for expression of the cloned nitrile hydratase gene cluster. J Gen Microbiol 138: 1003-1010
6. Hashimoto Y, Nishiyama M, Horinouchi S, Beppu T (1994) Nitrile hydratase gene from Rhodococcus sp. N-774: requirement for its downstream region for efficient expression. Biosci Biotechnol Biochem 58: 1859-1865
7. Ikehata O, Nishiyama M, Horinouchi S, Beppu T (1989) Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli. Eur J Biochem 181: 563-570
8. Kobayashi M, Yamada H (1996) Nitrile hydratase and its application to industrial production of acrylamide. Biosci Biotechnol Biochem 60: 1391-1400
9. Kobayashi M, Nishiyama M, Nagasawa T, Horinouchi S, Beppu T, Yamada H (1991) Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous Jl. Biochim Biophys Acta 1129: 23-33
10. Kobayashi M, Nagasawa T, Yamada H (1992) Enzymatic synthesis of acrylamide: a success story not yet over. Trends Biotechnol 10: 402-408
11. Komeda H, Kobayashi M, Shimizu S (1996) Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous Jl. Proc Natl Acad Sci USA 93: 4267-4272
12. Nagasawa T, Takeuchi K, Yamada H (1991) Characterization of a new cobalt-containing nitrile hydratase purified from urea-induced cells of Rhodococcus rhodochrous Jl. Eur J Biochem 196: 581-589
13. Nishiyama M, Horinouchi S, Kobayashi M, Nagasawa T, Yamada H, Beppu T (1991) Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23. J Bacteriol 173: 2465-2472
14. Stevenson DE, Feng R, Dumas F, Groleau D, Mihoc A, Storer AC (1992) Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase. Biotechnol Appl Biochem 15: 283-302
15. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33: 103-119