Heterologous expression of human transketolase

International Journal of Biochemistry and Cell Biology

Volumn 30, Issue 3, 1998, Pages 369-378

  Schenk, Gerhard ^a   Duggleby, Ronald G ^a   Nixon, Peter F ^a  

^a Funct Eng   (Australia)

Author keywords

Heterologous expression; Kinetic parameters; Protein purification; Recombinant human transketolase; Thiamin diphosphate

Indexed keywords

COCARBOXYLASE; MAGNESIUM ION; RECOMBINANT ENZYME; RIBOSE 5 PHOSPHATE; TRANSKETOLASE; UNCLASSIFIED DRUG; XYLULOSE 5 PHOSPHATE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HUMAN; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; WERNICKE KORSAKOFF SYNDROME;

CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; KINETICS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSKETOLASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0031831425     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(97)00154-4     Document Type: Article

References (34)

1. Abedinia M., Layfield R., Jones S.M., Nixon P.F., Mattick J.S. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem. Biophys. Res. Commun. 183:1992;1159-1166.
2. Berthon H.A., Kuchel P.W., Nixon P.F. High control coefficient of transketolase in the nonoxidative pentose phosphate pathway of human erythrocytes: NMR, antibody, and computer simulation studies. Biochemistry. 31:1992;12792-12798.
3. Blass J.P., Gibson G.E. Abnormality of a thiamine-requiring enzyme in patients with Wernicke-Korsakoff syndrome. N. Engl. J. Med. 297:1977;1367-1370.
4. C.K. Booth, Studies on vitamin K and thiamin, Ph.D. Thesis, The University of Queensland, Brisbane, Australia, 1991.
5. Booth C.K., Nixon P.F. Reconstitution of holotransketolase is by a thiamin-diphosphate-magnesium complex. Eur. J. Biochem. 218:1993;261-265.
6. H.A. de Boer, R.A. Kastelein, Biased codon usage: An exploration of its role in optimization of translation, in: W.S. Reznikoff, L. Gold (Eds.), Maximizing Gene Expression, Butterworth Publishers, New York, 1986, pp. 225-285.
7. Diefenbach R.J., Duggleby R.G. Pyruvate decarboxylase from Zymomonas mobilis: Structure and re-activation of apoenzyme by the cofactors thiamin diphosphate and magnesium ion. Biochem. J. 276:1991;439-445.
8. Duggleby R.G. Regression analysis of nonlinear Arrhenius plots: An empirical model and a computer program. Comput. Biol. Med. 14:1984;447-455.
9. Egan R.M., Sable H.Z. Transketolase kinetics: The slow reconstitution of the holoenzyme is due to rate-limiting dimerization of the subunits. J. Biol. Chem. 256:1981;4877-4883.
10. Elvin C.M., Thompson P.R., Argall M.E., Hendry P., Stamford N.P.J., Lilley P.E., Dixon N.E. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene. 87:1990;123-126.
11. Hawkins C.F., Borges A., Perham R.N. A common structural motif in thiamin pyrophosphate-binding enzymes. FEBS Lett. 255:1989;77-82.
12. Heinrich P.C., Noack K., Wiss O. A circular dichroism study of transketolase from baker's yeast. Biochem. Biophys. Res. Commun. 44:1971;275-279.
13. Heinrich P.C., Wiss O. Transketolase from human erythrocyte: Purification and properties. Helv. Chim. Acta. 54:1971;2658-2668.
14. Jung E.H., Sheu K.F.R., Blass J.P. Molecular cloning of human transketolase: Complementary DNA and protein sequence. FASEB J. 7:1993;A838.
15. Kochetov G.A. Structure and mechanism of action of transketolase. Biokhimiya. 51:1986;2020-2029.
16. Littlechild J.A., Turner N.J., Hobbs G.R., Lilly M.D., Rawas R., Watson W. Crystallization and preliminary X-ray crystallographic data with Escherichia coli transketolase. Acta Cryst. D. 51:1995;1074-1076.
17. McCool B.A., Plonk S.G., Martin P.R., Singleton C.K. Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals. J. Biol. Chem. 268:1993;1397-1404.
18. Nikkola M., Lindqvist Y., Schneider G. Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 Å resolution. J. Mol. Biol. 238:1994;387-404.
19. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.
20. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
21. Schaaf-Gerstenschläger I., Miosga T., Zimmermann F.K. Genetics of pentose-phosphate pathway enzymes in Saccharomyces cerevisiae. Bioresource Technol. 50:1995;59-64.
22. G. Schenk, Studies on the thiamin-dependent enzymes transketolase and pyruvate decarboxylase, Ph.D. Thesis, The University of Queensland, Brisbane, Australia, 1997.
23. Schenk G., Layfield R., Candy J.M., Duggleby R.G., Nixon P.F. Molecular evolutionary analysis of the thiamin diphosphate-dependent enzyme, transketolase. J. Mol. Evol. 44:1997;552-572.
24. Senac T., Hahn-Hägerdahl B. Effects of increased transaldolase activity on D-xylulose and D-glucose metabolism in Saccharomyces cerevisiae cell extracts. Appl. Environ. Microbiol. 57:1991;1701-1706.
25. Singleton C.K., Pekovich S.R., McCool B.A., Martin P.R. The thiamine-dependent hysteretic behaviour of human transketolase: Implications for thiamine deficiency. J. Nutr. 125:1995;189-194.
26. Singleton C.K., Wang J.J.L., Shan L., Martin P.R. Conserved residues are functionally distinct within transketolases of different species. Biochemistry. 35:1996;15865-15869.
27. Smeets E.H.J., Muller H., de Wael J. A NADH-dependent transketolase assay in erythrocyte hemolysates. Clin. Chim. Acta. 33:1971;379-386.
28. Sprenger G.A., Schörken U., Sprenger G., Sahm H. Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains. Eur. J. Biochem. 230:1995;525-532.
29. Sundström M., Lindqvist Y., Schneider G., Hellman U., Ronne H. Yeast TKL1 gene encodes a transketolase that is required for efficient glycolysis and biosynthesis of aromatic amino acids. J. Biol. Chem. 268:1993;24346-24352.
30. Tate J.R., Nixon P.F. Measurement of Michaelis constant for human erythrocyte transketolase and thiamin diphosphate. Anal. Biochem. 160:1987;78-87.
31. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22:1994;4673-4680.
32. M. Waltham, Studies on dihydrofolate reductase and transketolase, Ph.D. Thesis, The University of Queensland, Brisbane, Australia, 1990.
33. Wikner C., Meshalkina L., Nilsson U., Nikkola M., Lindqvist Y., Schneider G. Analysis of an invariant cofactor-protein interaction in thiamin diphosphate dependent enzymes by site-directed mutagenesis. J. Biol. Chem. 269:1994;32144-32150.
34. Wikner C., Meshalkina L., Nilsson U., Bäckström S., Lindqvist Y., Schneider G. His103 in yeast transketolase is required for substrate recognition and catalysis. Eur. J. Biochem. 233:1995;750-755.