Mutation detection by a two-hybrid assay

Human Molecular Genetics

Volumn 7, Issue 6, 1998, Pages 1029-1032

  Schwartz, Hillel ^a   Alvares, Christopher P ^b   White, Marga B ^b   Fields, Stanley ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Oncormed Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ARTICLE; DNA SEQUENCE; GENE MUTATION; GENETIC RECOMBINATION; HETEROZYGOSITY; HOMOZYGOSITY; HUMAN; HUMAN CELL; HYBRID; HYBRID GENE; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR GENE; YEAST CELL;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, P53; GENETIC TECHNIQUES; HUMANS; MUTATION; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 0031831318     PISSN: 09646906     EISSN: None     Source Type: Journal    
DOI: 10.1093/hmg/7.6.1029     Document Type: Article

References (16)

1. Hacia, J.G., Brody, L.C., Chee, M.S., Fodor, S.P.A. and Collins, F.S. (1996) Detection of heterozygous mutations in BRCAI using high density oligonucleotide arrays and two-colour fluorescence analysis. Nature Genet., 14, 441-447.
2. Ishioka, C., Frebourg, T., Vidal, Y.X., Vidai, M., Friend, S.H., Schmidt. S. and Iggo, R. (1993) Screening patients for heterozygous p53 mutations using a functional assay in yeast. Nature Genet., 5, 124-129.
3. Chen, L. and Powers, S. (1995) RAS in yeast: complementation assays for test of function. Methods Enzymol., 255, 465-468.
4. Ishioka, C., Suzuki, T., FitzGerald, M., Krainer, M., Shimodaira, H., Shimada, A., Nomizu, T., Isselbacher, K.J., Haber, D. and Kanamaru, R. (1997) Detection of heterozygous truncating mutations in the BRCAI and APC genes by using a rapid screening assay in yeast. Proc. Natl Acad. Sci. USA, 94, 2449-2453.
5. Fields, S. and Song, O.-K. (1989) A novel genetic system to detect protein-protein interactions. Nature, 340, 245-246.
6. Iwabuchi, K., Bartel, P.L., Li, B., Marraccino, R. and Fields, S. (1994) Two cellular proteins that bind to wild type but not mutant p53. Proc. Natl Acad. Sci. USA, 91, 6098-6102.
7. Reymond, A. and Brent, R. (1995) p16 proteins from melanoma-prone families are deficient in binding to Cdk4. Oncogene, 11, 1173-1178.
8. Durfee, T., Becherer, K., Chen, P.-L., Yeh, S.H., Yang, Y., Kilbum, A.E., Lee, W.-H. and Elledge, S.J. (1993) The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev., 7, 555-569.
9. Flaman, J.M. et al. (1995) A simple p53 functional assay for screening cell lines, blood, and tumors. Proc. Natl Acad. Sci. USA, 92, 3963-3967.
10. Thukral, S.K., Blain, G.C., Chang, K.H. and Fields, S. (1994) Distinct residues of human p53 implicated in binding to DNA, SV40 large T antigen. Mol. Cell. Biol., 14, 8315-8321.
11. Wu, L.C., Wang, Z.W., Tsan, J.T., Spillman, M.A., Phung, A., Xu, X.L., Yang, M.C., Hwang. L.Y., Bowcock, A.M. and Baer, R. (1996) Identification of a RING protein that can interact in vivo with the BRCAI gene product. Nature Genet., 14, 430-440.
12. Wong, A.K.C., Pero, R., Ormonde, P.A., Tavtigian, S.V. and Bartel, P.L. (1997) RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2. J. Biol. Chem., 272, 31941-31944.
13. Bartel, P.L. and Fields, S. (1995) Analyzing protein-protein interactions using two-hybrid system. Methods Enzymol., 254, 241-263.
14. Sherman, E. Fink, G.R. and Hicks, J.B. (1986) Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Chen, D.C., Yang, B.C. and Kuo, T.T. (1992) One-step transformation of yeast in stationary phase. Curr. Genet., 21, 83-84.
16. Hoffman, C.S. and Winston, F. (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267-272.