Characterization of second site mutations show that fast proton transfer to Q(B)- is restored in bacterial reaction centers of Rhodobacter sphaeroides containing the Asp-L213 → Asn lesion

Photosynthesis Research

Volumn 55, Issue 2-3, 1998, Pages 281-291

  Paddock, M L ^a   Senft, M E ^a   Graige, M S ^a   Rongey, S H ^a   Turanchik, T ^a   Feher, G ^a   Okamura, M Y ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bacterial photosynthesis; Electron transfer; Proton transfer; Rhodobacter sphaeroides

Indexed keywords

BACTERIA (MICROORGANISMS); RHODOBACTER SPHAEROIDES;

EID: 0031828474     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1005953615604     Document Type: Conference Paper

References (46)

1. Breton J and Verméglio A (eds) (1988) The Photosynthetic Bacterial Reaction Center, Structure and Dynamics Plenum Press, New York
2. Cao Y, Varo G, Chang M, Ni B, Needleman R and Lanyi JK (1991) Water is required for proton transfer from aspartate-96 to the bacteriorhodopsin Schiff base. Biochemistry 30: 10972-10979
3. Ermler U, Fritzsch G, Buchanan SK and Michel H (1994) Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.65 Å resolution: Cofactors and protein-cofactor interactions. Structure 2: 925-936
4. Feher G, Allen JP, Okamura MY and Rees DC (1989) Primary processes in bacterial photosynthesis: Structure and function of reaction centers. Nature 339: 111-116
5. Giangiacomo KM and Dutton PL (1989) In photosynthetic reaction centers, the free energy difference for electron transfer between quinones bound at the primary and secondary quinone-binding sites governs the observed secondary site specificity. Proc Natl Acad Sci USA 86: 2658-2662
6. B) Reduction in reaction centers of Rb. sphaeroides. J Am Chem Soc 118: 9005-9016
7. - in Asp-L213 → Asn mutant RCs from Rb. sphaeroides. Biophys J 70: 11 (abstr.)
8. Hatanaka M, Sasaki J, Kandori H, Ebrey TG, Needleman R, Lanyi JK and Maeda A (1996) Effects of arginine-82 on the interactions of internal water molecules in bacteriorhodopsin. Biochemistry 35: 6308-6312
9. Hanson DK, Baciou L, Tiede DM, Nance SL, Schiffer M and Sebban P (1992) In bacterial reaction centers protons can diffuse to the secondary quinone by alternative pathways. Biochim Biophys Acta 1102: 260-265
10. Hanson DK, Tiede DM, Nance SL, Chang CH and Schiffer M (1993) Site-specific and compensatory mutations imply unexpected pathways for proton delivery to the QB binding site of the photosynthetic reaction center. Proc Natl Acad Sci USA 90: 8929-8933
11. Heck RW, Boriack-Sjodin PA, Qian MZ, Tu CK, Christianson DW, Laipis PJ and Silverman DN (1996) Structure-based design of an intramolecular proton transfer site in murine carbonic anhydrase V. Biochemistry 35: 11605-11611
12. Humphrey W, Logunov I, Schulten K and Sheves M (1994) Molecular dynamics study of bacteriorhodopsin and artificial pigments. Biochemistry 33: 3668-3678
13. -. Biochim Biophys Acta 766: 126-140
14. 2-. Biochim Biophys Acta 809: 291-310
15. - to DQAQB in bacterial reaction centers from Rhodobacter sphaeroides. J Phys Chem 98: 3417-3423
16. A Site. Chem Phys 197: 355-366
17. Lancaster CRD, Ermler U and Michel H (1995) In: 'Anoxygenic Photosynthetic Bacteria, Blankenship RE, Madigan MT and Bauer CE (eds) pp 503-526. Kluwer Academic Publishers, Dordrecht, the Netherlands
18. Lanyi JK (1993) Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsin. Biochim Biophys Acta 1183: 241-261
19. Le Coutre J, Tittor J, Oesterhelt D and Gerwert K. (1995) Experimental evidence for hydrogen-bonded network proton transfer in bacteriorhodopsin shown by Fourier-transform infrared spectroscopy using azide as catalyst. Proc Natl Acad Sci USA 92: 4962-4966
20. B in the photosynthetic reaction center of Rhodopseudomonas viridis. Biochemistry 32: 1958-1964
21. Nabedryk E, Breton J, Hienerwadel R, Fogel C, Mantele, W, Paddock, ML and Okamura MY (1995) Fourier Transform Infrared Difference spectroscopy of secondary quinone acceptor photoreduction in proton transfer mutants of Rhodobacter sphaeroides. Biochemistry 34: 14722-14732
22. Okamura MY, Debus RJ, Kleinfeld D and Feher G (1982) Quinone binding sites in reaction centers from photosynthetic bacteria. In: Trumpower BL (ed) Functions of Quinones in Energy Conserving Systems, Ch V, pp 299-317. Academic Press, New York
23. Okamura MY and Feher G (1992) Proton transfer in reaction centers from photosynthetic bacteria. Annu Review Biochem. 61: 861-896
24. Okamura MY, Paddock ML, McPherson PH, Rongey SH and Feher G (1992) Proton transfer in bacterial reaction centers: Second site mutations Asn M44 to Asp or Arg M233 to Cys restore photosynthetic competence to Asp L213 to Asn mutants in RCs from Rb. Sphaeroides. In: 'Research in Photosynthesis;, Murata, N (ed) pp 349-356. Kluwer Academic Publishers, Dordrecht, the Netherlands
25. B in reaction centers from photosynthetic bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 577-594. Kluwer Academic Publishers, Dordrecht, the Netherlands
26. Paddock ML, Rongey SH, Abresch EC, Feher G and Okamura MY (1988) Reaction centers from three herbicide resistant mutants of Rhodobacter sphaeroides 2.4.1: Sequence analysis and preliminary characterization. Photosynth Res 17: 75-96
27. Paddock ML, Rongey SH, Feher G and Okamura MY (1989) Pathway of proton transfer in bacterial reaction centers: Replacement of glutamic acid 212 in the L subunit by glutamine inhibits quinone (secondary acceptor) turnover. Proc Natl Acad Sci USA 86: 6602-6606
28. Paddock ML, McPherson PH, Feher G and Okamura MY (1990) Pathway of proton transfer in bacterial reaction centers: Replacement of serine-L223 by alanine inhibits electron and proton transfers associated with the reduction of quinone to dihydroquinone. Proc Natl Acad Sci USA 87: 6803-6807
29. Paddock ML, Rongey SH, McPherson PH, Juth A, Feher G and Okamura MY (1994) Pathway of proton transfer in bacterial reaction centers: The role of Asp-L213 in proton transfers associated with the reduction of quinone to dihydroquinone. Biochemistry 36: 14238-14249
30. B by interchanging Asp and Glu at the L212 and L213 sites. Biochemistry 33 (submitted)
31. Ren XL, Tu CK, Laipis PJ and Silverman DN (1995) Proton transfer by histidine 67 in site-directed mutants of human carbonic anhydrase III. Biochemistry 34: 8492-8498
32. Rongey SH, Paddock ML, Feher G and Okamura MY (1993) Pathway of proton transfer in bacterial reaction centers: Second site mutation Asn-M44 to Asp restores electron and proton transfer in reaction centers from the photosynthetically deficient Asp-L213 to Asn mutant of Rhodobacter sphaeroides. Proc Natl Acad Sci USA 90: 1325-1329
33. Rongey SH, Juth A, Paddock ML, Feher G and Okamura MY (1995) Importance of a carboxylic acid at H173 for proton-coupled electron transfer in RCs of Rb. sphaeroides. Biophys J 68: 247 (abstr.)
34. Sambrook J, Fritzsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor
35. Sanger F, Nicklen S and Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
36. Sebban P, Maróti P, Schiffer M and Hanson DK (1995) Electrostatic dominoes: Long distance propagation of mutational effects in photosynthetic reaction centers of Rhodobacter capsulatus. Biochemistry 34: 8390-8397
37. Senft ME, Paddock ML, Rongey SH, Feher G and Okamura MY (1995) Suppressor mutation in the H-Subunit partially restores proton transfer in Asp-L213 to Asn mutant reaction centers from Rhodobacter sphaeroides. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol 1, pp 639-642. Kluwer academic Publishers, Dordrecht, the Netherlands
38. Silverman DN, Tu CK, Chen X, Tanhauser SM, Kresge AJ and Laipis PJ (1993) Rate-equilibria relationships in intramolecular proton transfer in human carbonic anhydrase III. Biochemistry 32: 10757-10762
39. Stowell MHB, McPhillips TM, Rees DC, Soltis SM, Abresch E and Feher G (1997) Light induced structural changes and the mechanism of electron/proton transfer in the photosynthetic reaction center. Science 276: 812-816
40. Swallow AJ (1982) In: Trumpower BL (ed) Function of quinones in energy conserving systems, pp 59-72. Academic Press, New York
41. Takahashi E and Wraight CA (1990) A crucial role for Asp-L213 in the proton transfer pathway to the secondary quinone of reaction centers from Rhodobacter sphaeroides. Biochim Biophys Acta 1020: 107-111
42. B-binding site of Rhodobacter sphaeroides reaction center. FEBS Lett 283: 140-144
43. B binding site. Biochemistry 31: 855-866
44. Takahashi E and Wraight CA (1996) Potentiation of proton transfer function by electrostatic interactions in photosynthetic reaction centers from Rhodobacter sphaeroides: First results from sitedirected mutation of the H subunit. Proc Natl Acad Sci USA 93: 2640-2645
45. Verméglio A and Clayton RK (1977) Kinetics of electron transfer between the primary and the secondary electron acceptor in reaction centers from Rhodopseudomonas sphaeroides. Biochim Biophys Acta 461: 159-165
46. Williams JC, Steiner LA, Ogden RC, Simon MI and Feher G (1983) Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides. Proc Natl Acad Sci USA 80: 6505-6509