Shortened PCR cycles in a conventional thermal cycler

BioTechniques

Volumn 25, Issue 2, 1998, Pages 208-210

  Mai, M ^a   Grabs, R ^a   Barnes, R D ^a   Vafiadis, P ^a   Polychronakos, C ^a,b  

^a RESEARCH INSTITUTE OF THE MCGILL UNIVERSITY HEALTH CENTRE   (Canada)

^b MONTREAL CHILDREN S HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; SOMATOMEDIN B; SOMATOMEDIN B RECEPTOR;

ARTICLE; DNA DENATURATION; DNA TEMPLATE; POLYMERASE CHAIN REACTION; TEMPERATURE; THERMAL CYCLER;

EID: 0031827985     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98252bm06     Document Type: Article

References (5)

1. Innis, M.A., K,B. Myambo, D.H. Gelfand and M.A.D. Brow. 1988. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of PCR-amplified DNA. Proc. Natl. Acad. Sci. USA 85:9436-9440.
2. Wetmer, J.G. and N. Davidian. 1968. Kinetics of renaturation of DNA. J. Mol. Biol. 31:349-370.
3. Wittwer, C.T. and D.J. Garling. 1991. Rapid cycle DNA amplification: time and temperature optimization. BioTechniques 10:76-83.
4. Wittwer, C.T., K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry and U.J. Balis. 1997. The LightCycler™: a microvolume multisample fluorimeter with rapid temperature control, BioTechniques 22:176-181.
5. Yap, E.P. and J.O. McGee. 1991. Short PCR product yields improved by lower denaturation temperatures. Nucleic Acids Res. 19:1713.