Preparation of shoot apex of Helianthus annuus L. for analysis with scanning electron microscope

Journal of Electron Microscopy

Volumn 47, Issue 2, 1998, Pages 179-182

  De Almeida, Julieta Andrea Silva ^a   Carvalho, Wagner Alves ^b   Pereira, Maria De Fátima D A ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b IQ   (Brazil)

Author keywords

Apex without pre treatment; Critical point drying; Floral apex of sunflower; Liquid substitution with glycerol; SEM viewing

Indexed keywords

HELIANTHUS ANNUUS;

SCANNING ELECTRON MICROSCOPY;

APEX WITHOUT PRE-TREATMENT; CONDITION; CRITICAL POINT DRYING; FLORAL APEX OF SUNFLOWER; HELIANTHUS ANNUUS L; LIQUID SUBSTITUTION WITH GLYCEROL; PRE-TREATMENTS; SCANNING ELECTRONS; SEM VIEWING; SHOOT APEX;

GLYCEROL;

EID: 0031824504     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023576     Document Type: Article

References (15)

1. Goodhew P J and Humphreys F J (1988) Electron Microscopy and Analysis, (Taylor & Francis).
2. Goldstein J I et al. (1992) Scanning Electron Microscopy and X-Ray Microanalysis, 2nd edition, (Plenum Press).
3. Lavoie D M, Little B J, Ray R I, Bennett RH, Lambert M W, Asper V, and Baerwalds R J (1994) Environmental scanning microscopy of marine aggregates. J Microscop. 178: 101-106.
4. Falk R H, Gifford E M Jr, and Cutter E G (1971) The effect of various fixations schedules on the scanning electron microscopic image of Tropaeolum majus. Am. J. Bot. 58: 676-680.
5. Dale J E and Pereira M F A (1979) Growth regulators and leaf development in Phaseolus vulgaris L. Revta Brasil Bot. 2: 77-86.
6. Pereira M F A and Dale J E (1982) Effects of 2,4-dichlorophenoxyacetic acid on shoot apex development of Phaseolus vulgaris. Z. Pflazenphysiol. 107: 169-177.
7. Sass J E (1951) Botanical Microthecnique, 2nd edition, (The Iowa State University Press, Iowa).
8. Horridge G A and Tamm S L (19XX) Critical point drying for scanning electron microscope study of ciliary motion. Science 163: 817-818.
9. Ensikat H J and Barthlott W (1993) Liquid substitution: a versatile procedure for SEM specimen preparation of biological materials without drying or coating. J. Microsc. 172: 195-2.
10. Lord E M and Eckard K J (1985) Shoot development in Citrus sinensis L. (Washington navel orange). I Floral and inflorescence ontogeny. Bot. Gaz. 146: 320-326.
11. Arai M, Saito T, Kaneko Y, and Matsushima H (1996) Observation of flower bud formation by a simple direct SEM viewing method. J. Electron Microsc. 45: 168-170.
12. Lagôa A M M A and Pereira M F D A (1984) Efeito correlativo de órgãos na iniciação floral de Phaseolus vulgaris cv. Goiano Precoce. In: Anais IV Congresso SBSP, pp. 1-6, (SBSP, Taubaté).
13. Leal C B F (1995) Floração em Euphorbia heterophylla L. Tese de Mestrado. Universidade Estadual de Campinas, p. 68.
14. Gama LHP (1996) Controle ambiental e hormonal do desenvolvimento do botão floral de Rhododendron sinsii (Azalea). Tese de Mestrado. Universidade Estadual de Campinas, p. 73.
15. Saito T, Hirosawa T, Horiuchi S, Murakami A, and Matsushima H (1995) A study of SEM examination on fresh hop (Humulus lupulus L.) peltate glandular trichomes. J. Electron Microsc. 44: 39-44.